ProFi Achievements
Major publications
The Human Proteome Project reports 90% completion of the human proteome
Along with the publication of the "Blueprint" map of the Human Proteome in Nature Communications, JPR has just published a "virtual issue" of a selection of 60 publications on the human proteome celebrating 10 years of the Human Proteome Project....
Proline: an efficient and user-friendly software suite for large-scale proteomics
We are pleased and very proud to announce the publication of Proline in Bioinformatics.
Here, we present Proline, a robust software suite for analysis of MS-based proteomics data which collects, processes, allows visualization and publication of proteomics datasets. We illustrate its ease of use for various steps in the validation and quantification workflow, its data curation capabilities and its computational efficiency….
Feel free to try the software by downloading the zero installation version.
ProteoRE, a web application for the discovery of biomarkers of diagnostic interest
Although biomarkers are widely used, important limitations have been identified due to their lack of specificity. An a priori knowledge-based approach (i.e. based on mechanistic criteria specific to the genesis of a disease) that takes advantage of ever-increasing “omics” databases could be a powerful way to identify biomarkers, provided that scientists have access to computer tools to search the data even when they have little experience in programming or bioinformatics support.
HDX-Viewer: interactive 3D visualization of hydrogen-deuterium exchange data
Bouyssié D, Lesne J, Locard-Paulet M, Albigot R, Burlet-Schiltz O, Marcoux J. Bioinformatics. 2019 Jul 9. pii: btz550. doi: 10.1093/bioinformatics/btz550. With the advent of fully automated sample preparation robots for Hydrogen–Deuterium eXchange coupled to Mass...
Structure of bacteriocins produced by the human gut symbiont revealed by Top-Down MS
In-vivo and in-vitro produced bacteriocin samples were analyzed by Top-Down MS to characterize the thioether bridge arrangement of a new class of sactipeptides produced by the gut symbiont. This original structure confers activity against pathogenic Clostridia and other multidrug-resistant strains and have no toxicity towards eukaryotic cells. This bacteriocin should be considered as a valuable candidate for drug development.
Integrative multi-OMICS analyses unveil STAT1 as a potential modifier gene in mevalonate kinase deficiency
We provide a proteogenomics analysis pipeline that takes advantage of subject-specific genomic and transcriptomic information to improve protein identification and hence advance individualised medicine.
Native MS for the determination of R2TP subcomplexes stoichiometries
R2TP is an HSP90 co-chaperone that assembles important macro-molecular machineries. It is composed of an RPAP3-PIH1D1 heterodimer, which binds the two essential AAA+ATPases RUVBL1/RUVBL2.
VisioProt-MS facilitates top-down data visualisation and analysis
This new web application offers a user-friendly interface for dynamic inspection and direct comparison of topdown proteomics data.
Proteomic characterization of the emerging Pandoraviridae family
With their huge DNA genomes packed in amphora-shaped particles of bacterium-like dimension, the Acanthamoeba-infecting Pandoraviridae remained the most spectacular and intriguing viruses since their 1st description in 2013.
Mass spectrometry analysis of IL-33 proteolytic maturation
IL-33 is a mediator of allergic inflammation and is localized in mucosal tissues to respond rapidly to environmental insults. These include allergens themselves, which can directly activate IL-33 through their intrinsic proteolytic activity. We used mass spectrometry to characterize maturation processes induced by allergens, involved in activation of IL-33 and asthma.
N-terminal oriented proteomics approach
Mass-spectrometry based proteomics combined with N-terminal chemical labelling using TMPP allows the characterization of proteolytic cleavage sites of Persephone by microbial proteases
Top 3-ID-DIA method
The MS Top 3-ID-DIA method allows global Host Cell Proteins (HCP) profiling and absolute quantification of key HCP within a single analysis, and therefore constitutes a promising alternative to classical ELISA kits to support bioprocess development.
cHPP Paper: sperm proteomics
In the context of the C-HPP worldwide effort, ProFI as a partner of the French-Swiss consortium, contributed to the targeted search of 38 theoretically detectable missing proteins (MPs) from chromosomes 2 and 14 various sperm fractions from a total of 15 healthy donors.