Although biomarkers are widely used, important limitations have been identified due to their lack of specificity. An a priori knowledge-based approach (i.e. based on mechanistic criteria specific to the genesis of a disease) that takes advantage of ever-increasing “omics” databases could be a powerful way to identify biomarkers, provided that scientists have access to computer tools to search the data even when they have little experience in programming or bioinformatics support.

Bouyssié D, Lesne J, Locard-Paulet M, Albigot R, Burlet-Schiltz O, Marcoux J. Bioinformatics. 2019 Jul 9. pii: btz550. doi: 10.1093/bioinformatics/btz550. With the advent of fully automated sample preparation robots for Hydrogen–Deuterium eXchange coupled to Mass...

In-vivo and in-vitro produced bacteriocin samples were analyzed by Top-Down MS to characterize the thioether bridge arrangement of a new class of sactipeptides produced by the gut symbiont. This original structure confers activity against pathogenic Clostridia and other multidrug-resistant strains and have no toxicity towards eukaryotic cells. This bacteriocin should be considered as a valuable candidate for drug development.  

We provide a proteogenomics analysis pipeline that takes advantage of subject-specific genomic and transcriptomic information to improve protein identification and hence advance individualised medicine.

R2TP is an HSP90 co-chaperone that assembles important macro-molecular machineries. It is composed of an RPAP3-PIH1D1 heterodimer, which binds the two essential AAA+ATPases RUVBL1/RUVBL2.

This new web application offers a user-friendly interface for dynamic inspection and direct comparison of topdown proteomics data.

With their huge DNA genomes packed in amphora-shaped particles of bacterium-like dimension, the Acanthamoeba-infecting Pandoraviridae remained the most spectacular and intriguing viruses since their 1st description in 2013.

IL-33 is a mediator of allergic inflammation and is localized in mucosal tissues to respond rapidly to environmental insults. These include allergens themselves, which can directly activate IL-33 through their intrinsic proteolytic activity. We used mass spectrometry to characterize maturation processes induced by allergens, involved in activation of IL-33 and asthma.

Mass-spectrometry based proteomics combined with N-terminal chemical labelling using TMPP allows the characterization of proteolytic cleavage sites of Persephone by microbial proteases

The MS Top 3-ID-DIA method allows global Host Cell Proteins (HCP) profiling and absolute quantification of key HCP within a single analysis, and therefore constitutes a promising alternative to classical ELISA kits to support bioprocess development.

In the context of the C-HPP worldwide effort, ProFI as a partner of the French-Swiss consortium, contributed to the targeted search of 38 theoretically detectable missing proteins (MPs) from chromosomes 2 and 14 various sperm fractions from a total of 15 healthy donors.