ProFi Achievements

Major publications

ProteoRE, a web application for the discovery of biomarkers of diagnostic interest

Although biomarkers are widely used, important limitations have been identified due to their lack of specificity. An a priori knowledge-based approach (i.e. based on mechanistic criteria specific to the genesis of a disease) that takes advantage of ever-increasing “omics” databases could be a powerful way to identify biomarkers, provided that scientists have access to computer tools to search the data even when they have little experience in programming or bioinformatics support.

Structure of bacteriocins produced by the human gut symbiont revealed by Top-Down MS

In-vivo and in-vitro produced bacteriocin samples were analyzed by Top-Down MS to characterize the thioether bridge arrangement of a new class of sactipeptides produced by the gut symbiont. This original structure confers activity against pathogenic Clostridia and other multidrug-resistant strains and have no toxicity towards eukaryotic cells. This bacteriocin should be considered as a valuable candidate for drug development.  

Mass spectrometry analysis of IL-33 proteolytic maturation

IL-33 is a mediator of allergic inflammation and is localized in mucosal tissues to respond rapidly to environmental insults. These include allergens themselves, which can directly activate IL-33 through their intrinsic proteolytic activity. We used mass spectrometry to characterize maturation processes induced by allergens, involved in activation of IL-33 and asthma.

N-terminal oriented proteomics approach

Mass-spectrometry based proteomics combined with N-terminal chemical labelling using TMPP allows the characterization of proteolytic cleavage sites of Persephone by microbial proteases

Top 3-ID-DIA method

The MS Top 3-ID-DIA method allows global Host Cell Proteins (HCP) profiling and absolute quantification of key HCP within a single analysis, and therefore constitutes a promising alternative to classical ELISA kits to support bioprocess development.

cHPP Paper: sperm proteomics

In the context of the C-HPP worldwide effort, ProFI as a partner of the French-Swiss consortium, contributed to the targeted search of 38 theoretically detectable missing proteins (MPs) from chromosomes 2 and 14 various sperm fractions from a total of 15 healthy donors.