ProFi Achievements
Publications
2020 |
Pirro, M; Rombouts, Y; Stella, A; Neyrolles, O; Burlet-Schiltz, O; van Vliet, S J; de Ru, A H; Mohammed, Y; Wuhrer, M; van Veelen, P A; Hensbergen, P J Characterization of Macrophage Galactose-type Lectin (MGL) ligands in colorectal cancer cell lines Article de journal Biochim Biophys Acta Gen Subj, 1864 (4), p. 129513, 2020. @article{pmid31911241, title = {Characterization of Macrophage Galactose-type Lectin (MGL) ligands in colorectal cancer cell lines}, author = {M Pirro and Y Rombouts and A Stella and O Neyrolles and O Burlet-Schiltz and S J van Vliet and A H de Ru and Y Mohammed and M Wuhrer and P A van Veelen and P J Hensbergen}, doi = {10.1016/j.bbagen.2020.129513}, year = {2020}, date = {2020-04-03}, journal = {Biochim Biophys Acta Gen Subj}, volume = {1864}, number = {4}, pages = {129513}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ibrahim, M; Ayoub, D; Wasselin, T; Dorsselaer, Van A; Maho, Le Y; Raclot, T; Bertile, F Alterations in rat adipose tissue transcriptome and proteome in response to prolonged fasting Article de journal Biological Chemistry, 401 (3), p. 389-405, 2020. @article{643, title = {Alterations in rat adipose tissue transcriptome and proteome in response to prolonged fasting}, author = {M Ibrahim and D Ayoub and T Wasselin and A Van Dorsselaer and Y Le Maho and T Raclot and F Bertile}, doi = {10.1515/hsz-2019-0184}, year = {2020}, date = {2020-02-25}, journal = {Biological Chemistry}, volume = {401}, number = {3}, pages = {389-405}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Bouyssié, D; Hesse, A M; Mouton-Barbosa, E; Rompais, M; Macron, C; Carapito, C; de Peredo, A G; Couté, Y; Dupierris, V; Burel, A; Menetrey, J P; Kalaitzakis, A; Poisat, J; Romdhani, A; Burlet-Schiltz, O; Cianférani, S; Garin, J; Bruley, C Proline: an efficient and user-friendly software suite for large-scale proteomics Article de journal Bioinformatics, 2020. @article{pmid32096818, title = {Proline: an efficient and user-friendly software suite for large-scale proteomics}, author = {D Bouyssié and A M Hesse and E Mouton-Barbosa and M Rompais and C Macron and C Carapito and A G de Peredo and Y Couté and V Dupierris and A Burel and J P Menetrey and A Kalaitzakis and J Poisat and A Romdhani and O Burlet-Schiltz and S Cianférani and J Garin and C Bruley}, doi = {10.1093/bioinformatics/btaa118}, year = {2020}, date = {2020-02-25}, journal = {Bioinformatics}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Bonnet, M; Soulat, J; Bons, J; Leger, S; Koning, De L; Carapito, C; Picard, B Quantification of biomarkers for beef meat qualities using a combination of Parallel Reaction Monitoring- and antibody-based proteomics Article de journal Food Chem, 317 , p. 126376., 2020. @article{641, title = {Quantification of biomarkers for beef meat qualities using a combination of Parallel Reaction Monitoring- and antibody-based proteomics}, author = {M Bonnet and J Soulat and J Bons and S Leger and L De Koning and C Carapito and B Picard}, doi = {10.1016/j.foodchem.2020.126376}, year = {2020}, date = {2020-02-10}, journal = {Food Chem}, volume = {317}, pages = {126376.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Locard-Paulet, M; Bouyssié, D; Froment, C; Burlet-Schiltz, O; Jensen, L J Comparing 22 Popular Phosphoproteomics Pipelines for Peptide Identification and Site Localization Article de journal J. Proteome Res., 19 (3), p. 1338–1345, 2020. @article{pmid31975593, title = {Comparing 22 Popular Phosphoproteomics Pipelines for Peptide Identification and Site Localization}, author = {M Locard-Paulet and D Bouyssié and C Froment and O Burlet-Schiltz and L J Jensen}, doi = {10.1021/acs.jproteome.9b00679}, year = {2020}, date = {2020-02-04}, journal = {J. Proteome Res.}, volume = {19}, number = {3}, pages = {1338--1345}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Imbert, C; Montfort, A; Fraisse, M; Marcheteau, E; Gilhodes, J; Martin, E; Bertrand, F; Marcellin, M; Burlet-Schiltz, O; Peredo, A G; Garcia, V; Carpentier, S; Tartare-Deckert, S; Brousset, P; Rochaix, P; Puisset, F; Filleron, T; Meyer, N; Lamant, L; Levade, T; Ségui, B; Andrieu-Abadie, N; Colacios, C Resistance of melanoma to immune checkpoint inhibitors is overcome by targeting the sphingosine kinase-1 Article de journal Nat Commun, 11 (1), p. 437, 2020. @article{pmid31974367, title = {Resistance of melanoma to immune checkpoint inhibitors is overcome by targeting the sphingosine kinase-1}, author = {C Imbert and A Montfort and M Fraisse and E Marcheteau and J Gilhodes and E Martin and F Bertrand and M Marcellin and O Burlet-Schiltz and A G Peredo and V Garcia and S Carpentier and S Tartare-Deckert and P Brousset and P Rochaix and F Puisset and T Filleron and N Meyer and L Lamant and T Levade and B Ségui and N Andrieu-Abadie and C Colacios}, doi = { 10.1038/s41467-019-14218-7}, year = {2020}, date = {2020-01-23}, journal = {Nat Commun}, volume = {11}, number = {1}, pages = {437}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Froment, C; Hourset, M; S?enz-Oyh?r?guy, N; Mouton-Barbosa, E; Willmann, C; Zanolli, C; Esclassan, R; Donat, R; Th?ves, C; Burlet-Schiltz, O; Mollereau, C Analysis of 5000 year-old human teeth using optimized large-scale and targeted proteomics approaches for detection of sex-specific peptides Article de journal J Proteomics, 211 , p. 103548, 2020. @article{pmid31626997, title = {Analysis of 5000 year-old human teeth using optimized large-scale and targeted proteomics approaches for detection of sex-specific peptides}, author = {C Froment and M Hourset and N S?enz-Oyh?r?guy and E Mouton-Barbosa and C Willmann and C Zanolli and R Esclassan and R Donat and C Th?ves and O Burlet-Schiltz and C Mollereau}, doi = {10.1016/j.jprot.2019.103548}, year = {2020}, date = {2020-01-16}, journal = {J Proteomics}, volume = {211}, pages = {103548}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Clement, E; Lazar, I; Attané, C; Carrié, L; Dauvillier, S; Ducoux-Petit, M; Estève, D; Menneteau, T; Moutahir, M; Gonidec, Le S; Dalle, S; Valet, P; Burlet-Schiltz, O; Muller, C; Nieto, L Adipocyte extracellular vesicles carry enzymes and fatty acids that stimulate mitochondrial metabolism and remodeling in tumor cells Article de journal EMBO J., 39 (3), p. e102525, 2020. @article{pmid31919869, title = {Adipocyte extracellular vesicles carry enzymes and fatty acids that stimulate mitochondrial metabolism and remodeling in tumor cells}, author = {E Clement and I Lazar and C Attané and L Carrié and S Dauvillier and M Ducoux-Petit and D Estève and T Menneteau and M Moutahir and S Le Gonidec and S Dalle and P Valet and O Burlet-Schiltz and C Muller and L Nieto}, doi = {10.15252/embj.2019102525}, year = {2020}, date = {2020-01-10}, journal = {EMBO J.}, volume = {39}, number = {3}, pages = {e102525}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2019 |
Bouyssié, D; Lesne, J; Locard-Paulet, M; Albigot, R; Burlet-Schiltz, O; Marcoux, J HDX-Viewer: interactive 3D visualization of hydrogen-deuterium exchange data Article de journal Bioinformatics, 35 (24), p. 5331–5333, 2019. @article{pmid31287496, title = {HDX-Viewer: interactive 3D visualization of hydrogen-deuterium exchange data}, author = {D Bouyssié and J Lesne and M Locard-Paulet and R Albigot and O Burlet-Schiltz and J Marcoux}, doi = {10.1093/bioinformatics/btz550}, year = {2019}, date = {2019-12-15}, journal = {Bioinformatics}, volume = {35}, number = {24}, pages = {5331--5333}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Santin, Y; Fazal, L; Sainte-Marie, Y; Sicard, P; Maggiorani, D; Tortosa, F; Y?cel, Y Y; Teyssedre, L; Rouquette, J; Marcellin, M; Vindis, C; Shih, J C; Lairez, O; Burlet-Schiltz, O; Parini, A; Lezoualc'h, F; Mialet-Perez, J Mitochondrial 4-ĦNE derived from MAO-A promotes mitoCa2+ overload in chronic postischemic cardiac remodeling Article de journal Cell Death Differ., 2019. @article{pmid31819159, title = {Mitochondrial 4-ĦNE derived from MAO-A promotes mitoCa2+ overload in chronic postischemic cardiac remodeling}, author = {Y Santin and L Fazal and Y Sainte-Marie and P Sicard and D Maggiorani and F Tortosa and Y Y Y?cel and L Teyssedre and J Rouquette and M Marcellin and C Vindis and J C Shih and O Lairez and O Burlet-Schiltz and A Parini and F Lezoualc'h and J Mialet-Perez}, doi = {10.1038/s41418-019-0470-y}, year = {2019}, date = {2019-12-09}, journal = {Cell Death Differ.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Baxter, P N W; Ouahabi, Al A; Karmazin, L; Varnek, A; Strub, J M; Cianferani, S An Investigation into the Stephens-Castro Synthesis of Dehydrotriaryl[12]annulenes: Factors Influencing the Cyclotrimerization Article de journal European Journal of Organic Chemistry, 40 , p. 6783-6795, 2019, (2019-02). @article{652, title = {An Investigation into the Stephens-Castro Synthesis of Dehydrotriaryl[12]annulenes: Factors Influencing the Cyclotrimerization}, author = {P N W Baxter and A Al Ouahabi and L Karmazin and A Varnek and J M Strub and S Cianferani}, doi = {10.1002/ejoc.201901053}, year = {2019}, date = {2019-10-18}, journal = {European Journal of Organic Chemistry}, volume = {40}, pages = {6783-6795}, note = {2019-02}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Barre, A; Pichereaux, C; Velazquez, E; Maudouit, A; Simplicien, M; Garnier, L; Bienvenu, F; Bienvenu, J; Burlet-Schiltz, O; Auriol, C; Benoist, H; Rougé, P Insights into the Allergenic Potential of the Edible Yellow Mealworm (Ŧenebrio molitor) Article de journal Foods, 8 (10), 2019. @article{pmid31635354, title = {Insights into the Allergenic Potential of the Edible Yellow Mealworm (Ŧenebrio molitor)}, author = {A Barre and C Pichereaux and E Velazquez and A Maudouit and M Simplicien and L Garnier and F Bienvenu and J Bienvenu and O Burlet-Schiltz and C Auriol and H Benoist and P Rougé}, doi = {10.3390/foods8100515}, year = {2019}, date = {2019-10-18}, journal = {Foods}, volume = {8}, number = {10}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Voisinne, G; Kersse, K; Chaoui, K; Lu, L; Chaix, J; Zhang, L; Menoita, Goncalves M; Girard, L; Ounoughene, Y; Wang, H; Burlet-Schiltz, O; Luche, H; Fiore, F; Malissen, M; de Peredo, Gonzalez A; Liang, Y; Roncagalli, R; Malissen, B Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics Article de journal Nat. Immunol., 20 (11), p. 1530–1541, 2019. @article{pmid31591574, title = {Quantitative interactomics in primary T cells unveils TCR signal diversification extent and dynamics}, author = {G Voisinne and K Kersse and K Chaoui and L Lu and J Chaix and L Zhang and M Goncalves Menoita and L Girard and Y Ounoughene and H Wang and O Burlet-Schiltz and H Luche and F Fiore and M Malissen and A Gonzalez de Peredo and Y Liang and R Roncagalli and B Malissen}, doi = {10.1038/s41590-019-0489-8}, year = {2019}, date = {2019-10-07}, journal = {Nat. Immunol.}, volume = {20}, number = {11}, pages = {1530--1541}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ayala-Nunez, NV.; Follain, G; Delalande, F; Hirschler, A; Partiot, E; Hale, GL.; Bollweg, BC.; Roels, J; Chazal, M; Bakoa, F; Carocci, M; Bourdoulous, S; Faklaris, O; Zaki, SR.; Eckly, A; Uring-Lambert, B; Doussau, F; Cianferani, S; Carapito, C; Jacobs, FMJ.; Jouvenet, N; Goetz, JG.; Gaudin, R Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells Article de journal Nat Commun. 2019 Sep 27;10(1):4430., 2019. @article{651, title = {Zika virus enhances monocyte adhesion and transmigration favoring viral dissemination to neural cells}, author = {NV. Ayala-Nunez and G Follain and F Delalande and A Hirschler and E Partiot and GL. Hale and BC. Bollweg and J Roels and M Chazal and F Bakoa and M Carocci and S Bourdoulous and O Faklaris and SR. Zaki and A Eckly and B Uring-Lambert and F Doussau and S Cianferani and C Carapito and FMJ. Jacobs and N Jouvenet and JG. Goetz and R Gaudin}, doi = {10.1038/s41467-019-12408-x}, year = {2019}, date = {2019-09-27}, journal = {Nat Commun. 2019 Sep 27;10(1):4430.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
S, Hessmann Erb Rabuka Huguet Josephs Beck Drake PM Cianférani Hernandez-Alba Houel S S D R J A S O A Case Study to Identify the Drug Conjugation Site of a Site-Specific Antibody-Drug-Conjugate Using Middle-Down Mass Spectrometry Article de journal J Am Soc Mass Spectrom., 30 (11), p. 2419-2429., 2019. @article{649, title = {A Case Study to Identify the Drug Conjugation Site of a Site-Specific Antibody-Drug-Conjugate Using Middle-Down Mass Spectrometry}, author = {Hessmann Erb Rabuka Huguet Josephs Beck Drake PM Cianférani S S D R J A S Hernandez-Alba O Houel S}, doi = {10.1007/s13361-019-02296-2}, year = {2019}, date = {2019-09-19}, journal = {J Am Soc Mass Spectrom.}, volume = {30}, number = {11}, pages = {2419-2429.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Schneider, F; Dureau, A -F; Hellé, S; Betscha, C; Senger, B; Cremel, G; Boulmedais, F; Strub, J M; Corti, A; Meyer, N; Guillot, M; Schaaf, P; Metz-Boutigue, M H A Pilot Study on Continuous Infusion of 4% Albumin in Critically Ill Patients: Impact on Nosocomial Infection via a Reduction Mechanism for Oxidized Substrates Article de journal Critical Care Explorations, 1 (9), 2019, (non). @article{653, title = {A Pilot Study on Continuous Infusion of 4% Albumin in Critically Ill Patients: Impact on Nosocomial Infection via a Reduction Mechanism for Oxidized Substrates}, author = {F Schneider and A -F Dureau and S Hellé and C Betscha and B Senger and G Cremel and F Boulmedais and J M Strub and A Corti and N Meyer and M Guillot and P Schaaf and M H Metz-Boutigue}, doi = {10.1097/CCE.0000000000000044}, year = {2019}, date = {2019-09-19}, journal = {Critical Care Explorations}, volume = {1}, number = {9}, note = {non}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Sabou, M; Doderer-Lang, C; Leyer, C; Konjic, A; Kubina, S; Lennon, S; Rohr, O; Viville, S; Cianférani, S; Candolfi, E; Pfaff, AW.; Brunet, J Toxoplasma gondii ROP16 kinase silences the cyclin B1 gene promoter by hijacking host cell UHRF1-dependent epigenetic pathways Article de journal Cell Mol Life Sci. 2019 Sep 6., 2019. @article{650, title = {Toxoplasma gondii ROP16 kinase silences the cyclin B1 gene promoter by hijacking host cell UHRF1-dependent epigenetic pathways}, author = {M Sabou and C Doderer-Lang and C Leyer and A Konjic and S Kubina and S Lennon and O Rohr and S Viville and S Cianférani and E Candolfi and AW. Pfaff and J Brunet}, doi = {10.1007/s00018-019-03267-2}, year = {2019}, date = {2019-09-06}, journal = {Cell Mol Life Sci. 2019 Sep 6.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Hartmann, L; Botzanowski, T; Galibert, M; Jullian, M; l Chabrol, E; Zeder-Lutz, G; Kugler, V; Stojko, J; Strub, JM.; Ferry, G; Frankiewicz, L; Puget, K; Wagner, R; Cianférani, S; Boutin, JA. VHH characterization. Comparison of recombinant with chemically synthesized anti-HER2 VHH Article de journal Protein Sci., 28 (10), p. 1865-1879, 2019. @article{648, title = {VHH characterization. Comparison of recombinant with chemically synthesized anti-HER2 VHH}, author = {L Hartmann and T Botzanowski and M Galibert and M Jullian and E l Chabrol and G Zeder-Lutz and V Kugler and J Stojko and JM. Strub and G Ferry and L Frankiewicz and K Puget and R Wagner and S Cianférani and JA. Boutin}, doi = {10.1002/pro.3712}, year = {2019}, date = {2019-08-29}, journal = {Protein Sci.}, volume = {28}, number = {10}, pages = {1865-1879}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chazarin, B; Ziemianin, A; Evans, A L; Meugnier, E; Loizon, E; Chery, I; Arnemo, J M; Swenson, J E; Gauquelin-Koch, G; Simon, C; Blanc, S; Lefai, E; Bertile, F Limited oxidative stress favors resistance to skeletal muscle atrophy in hibernating brown bears (Ursus arctos) Article de journal Antioxidants, 8 (9), p. 334, 2019, (2011/34). @article{647, title = {Limited oxidative stress favors resistance to skeletal muscle atrophy in hibernating brown bears (Ursus arctos)}, author = {B Chazarin and A Ziemianin and A L Evans and E Meugnier and E Loizon and I Chery and J M Arnemo and J E Swenson and G Gauquelin-Koch and C Simon and S Blanc and E Lefai and F Bertile}, doi = {10.3390/antiox8090334}, year = {2019}, date = {2019-08-22}, journal = {Antioxidants}, volume = {8}, number = {9}, pages = {334}, note = {2011/34}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Gervason, S; Larkem, D; Mansour, AB.; Botzanowski, T; Müller, CS.; Pecqueur, L; Pavec, Le G; Delaunay-Moisan, A; Brun, O; Agramunt, J; Grandas, A; Fontecave, M; Schünemann, V; Cianférani, S; Sizun, C; Tolédano, MB.; D'Autréaux, B Physiologically relevant reconstitution of iron-sulfur cluster biosynthesis uncovers persulfide-processing functions of ferredoxin-2 and frataxin Article de journal Nat Commun., 10 (1), p. 3566, 2019. @article{638, title = {Physiologically relevant reconstitution of iron-sulfur cluster biosynthesis uncovers persulfide-processing functions of ferredoxin-2 and frataxin}, author = {S Gervason and D Larkem and AB. Mansour and T Botzanowski and CS. Müller and L Pecqueur and G Le Pavec and A Delaunay-Moisan and O Brun and J Agramunt and A Grandas and M Fontecave and V Schünemann and S Cianférani and C Sizun and MB. Tolédano and B D'Autréaux}, doi = {10.1038/s41467-019-11470-9}, year = {2019}, date = {2019-08-08}, journal = {Nat Commun.}, volume = {10}, number = {1}, pages = {3566}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Sanchez, CP.; Cubel, Moliner S; Nyboer, B; Jankowska-Döllken, M; Schaeffer-Reiss, C; Ayoub, D; Planelles, G; Lanzer, M Phosphomimetic substitution at Ser-33 of the chloroquine resistance transporter PfCRT reconstitutes drug responses in Plasmodium falciparum Article de journal J Biol Chem., p. pii: jbc.RA119.009464., 2019. @article{646, title = {Phosphomimetic substitution at Ser-33 of the chloroquine resistance transporter PfCRT reconstitutes drug responses in Plasmodium falciparum}, author = {CP. Sanchez and S Moliner Cubel and B Nyboer and M Jankowska-Döllken and C Schaeffer-Reiss and D Ayoub and G Planelles and M Lanzer}, doi = {10.1074/jbc.RA119.009464}, year = {2019}, date = {2019-07-08}, journal = {J Biol Chem.}, pages = {pii: jbc.RA119.009464.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Quque, M; Benhaim-Delarbre, M; Deneubourg, JL.; Sueur, C; Criscuolo, F; Bertile, F Division of labour in the black garden ant (Lasius niger) leads to three distinct proteomes Article de journal J Insect Physiol., 117 , p. 103907, 2019, (2015/29). @article{644, title = {Division of labour in the black garden ant (Lasius niger) leads to three distinct proteomes}, author = {M Quque and M Benhaim-Delarbre and JL. Deneubourg and C Sueur and F Criscuolo and F Bertile}, doi = {10.1016/j.jinsphys.2019.103907}, year = {2019}, date = {2019-06-27}, journal = {J Insect Physiol.}, volume = {117}, pages = {103907}, note = {2015/29}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dalzon, B; Bons, J; Diemer, H; Collin-Faure, V; Marie-Desvergne, C; Dubosson, M; Cianferani, S; Carapito, C; Rabilloud, T A Proteomic View of Cellular Responses to Anticancer Quinoline-Copper Complexes Article de journal Proteomes, 7 (2), p. pii: E26, 2019. @article{639, title = {A Proteomic View of Cellular Responses to Anticancer Quinoline-Copper Complexes}, author = {B Dalzon and J Bons and H Diemer and V Collin-Faure and C Marie-Desvergne and M Dubosson and S Cianferani and C Carapito and T Rabilloud}, doi = {10.3390/proteomes7020026}, year = {2019}, date = {2019-06-24}, journal = {Proteomes}, volume = {7}, number = {2}, pages = {pii: E26}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chazarin, B; Storey, KB.; Ziemianin, A; Chanon, S; Plumel, M; Chery, I; Durand, C; Evans, AL.; Arnemo, JM.; Zedrosser, A; Swenson, JE.; Gauquelin-Koch, G; Simon, C; Blanc, S; Lefai, E; Bertile, F Metabolic reprogramming involving glycolysis in the hibernating brown bear skeletal muscle Article de journal Front Zool., 16 , p. 12, 2019. @article{645, title = {Metabolic reprogramming involving glycolysis in the hibernating brown bear skeletal muscle}, author = {B Chazarin and KB. Storey and A Ziemianin and S Chanon and M Plumel and I Chery and C Durand and AL. Evans and JM. Arnemo and A Zedrosser and JE. Swenson and G Gauquelin-Koch and C Simon and S Blanc and E Lefai and F Bertile}, doi = {10.1186/s12983-019-0312-2}, year = {2019}, date = {2019-05-06}, journal = {Front Zool.}, volume = {16}, pages = {12}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Plumel, M; Dumont, S; Maes, P; Sandu, C; Felder-Scmittbuhl, M P; Challet, E; Bertile, F Circadian Analysis of the Mouse Cerebellum Proteome Article de journal International Journal of Molecular Sciences, 20 (8), p. 1852, 2019, (2012/26). @article{631, title = {Circadian Analysis of the Mouse Cerebellum Proteome}, author = {M Plumel and S Dumont and P Maes and C Sandu and M P Felder-Scmittbuhl and E Challet and F Bertile}, doi = {10.3390/ijms20081852}, year = {2019}, date = {2019-04-15}, journal = {International Journal of Molecular Sciences}, volume = {20}, number = {8}, pages = {1852}, note = {2012/26}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Giroud, S; Chery, I; F., Bertile;; Bertrand-Michel, J; Tascher, G; Gauquelin-Koch, G; Arnemo, J M; Swenson, J E; Singh, N V; Lefai, E; Evans, A L; Simon, C; Blanc, S Lipidomics Reveals Seasonal Shifts in a Large-Bodied Hibernator, the Brown Bear Article de journal Front Physiol., 10 , p. 389, 2019, (2011/30). @article{630, title = {Lipidomics Reveals Seasonal Shifts in a Large-Bodied Hibernator, the Brown Bear}, author = {S Giroud and I Chery and Bertile; F. and J Bertrand-Michel and G Tascher and G Gauquelin-Koch and J M Arnemo and J E Swenson and N V Singh and E Lefai and A L Evans and C Simon and S Blanc}, doi = {10.3389/fphys.2019.00389}, year = {2019}, date = {2019-04-12}, journal = {Front Physiol.}, volume = {10}, pages = {389}, note = {2011/30}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Camus, M; Hirschi, S; Prevot, G; Chenard, M P; Mal, H; Stern, M; Reynaud-Gaubert, M; Gilhodes, J; Burlet-Schiltz, O; Brousset, P; Colombat, M Proteomic evidence of specific IGKV1-8 association with cystic lung light chain deposition disease Article de journal Blood, 133 (26), p. 2741–2744, 2019. @article{pmid30967366, title = {Proteomic evidence of specific IGKV1-8 association with cystic lung light chain deposition disease}, author = {M Camus and S Hirschi and G Prevot and M P Chenard and H Mal and M Stern and M Reynaud-Gaubert and J Gilhodes and O Burlet-Schiltz and P Brousset and M Colombat}, doi = {10.1182/blood.2019898577}, year = {2019}, date = {2019-04-09}, journal = {Blood}, volume = {133}, number = {26}, pages = {2741--2744}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chabert, M; Rousset, X; Colombat, M; Lacasa, M; Kakanakou, H; Bourderioux, M; Brousset, P; Burlet-Schiltz, O; Liepnieks, J J; Kluve-Beckerman, B; Lambert, G; Châtelet, F P; Benson, M D; Kalopissis, A D A transgenic mouse model reproduces human hereditary systemic amyloidosis Article de journal Kidney Int., 96 (3), p. 628–641, 2019. @article{pmid31200944, title = {A transgenic mouse model reproduces human hereditary systemic amyloidosis}, author = {M Chabert and X Rousset and M Colombat and M Lacasa and H Kakanakou and M Bourderioux and P Brousset and O Burlet-Schiltz and J J Liepnieks and B Kluve-Beckerman and G Lambert and F P Châtelet and M D Benson and A D Kalopissis}, doi = {10.1016/j.kint.2019.03.013}, year = {2019}, date = {2019-03-28}, journal = {Kidney Int.}, volume = {96}, number = {3}, pages = {628--641}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Bons, J; Macron, C; Aude-Garcia, C; Vaca-Jacome, SA.; Rompais, M; Cianférani, S; Carapito, C; Rabilloud, T A Combined N-terminomics and Shotgun Proteomics Approach to Investigate the Responses of Human Cells to Rapamycin and Zinc at the Mitochondrial Level Article de journal Mol Cell Proteomics, 18 (6), p. 1085-1095, 2019. @article{640, title = {A Combined N-terminomics and Shotgun Proteomics Approach to Investigate the Responses of Human Cells to Rapamycin and Zinc at the Mitochondrial Level}, author = {J Bons and C Macron and C Aude-Garcia and SA. Vaca-Jacome and M Rompais and S Cianférani and C Carapito and T Rabilloud}, doi = {10.1074/mcp.RA118.001269}, year = {2019}, date = {2019-03-15}, journal = {Mol Cell Proteomics}, volume = {18}, number = {6}, pages = {1085-1095}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Muller, L; Fornecker, L; Cianferani, S; Carapito, C Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics Article de journal Methods Mol Biol., 1959 , p. 123-127, 2019. @article{632, title = {Tube-Gel: A Fast and Effective Sample Preparation Method for High-Throughput Quantitative Proteomics}, author = {L Muller and L Fornecker and S Cianferani and C Carapito}, doi = {10.1007/978-1-4939-9164-8_8}, year = {2019}, date = {2019-03-10}, journal = {Methods Mol Biol.}, volume = {1959}, pages = {123-127}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Pichereaux, C; Laurent, E A; Gargaros, A; Viudes, S; Durieu, C; Lamaze, T; Grieu, P; Burlet-Schiltz, O J Proteomics, 200 , p. 28–39, 2019. @article{pmid30862563, title = {Analysis of durum wheat proteome changes under marine and fungal biostimulant treatments using large-scale quantitative proteomics: A useful dataset of durum wheat proteins}, author = {C Pichereaux and E A Laurent and A Gargaros and S Viudes and C Durieu and T Lamaze and P Grieu and O Burlet-Schiltz}, doi = {10.1016/j.jprot.2019.03.003}, year = {2019}, date = {2019-03-09}, journal = {J Proteomics}, volume = {200}, pages = {28--39}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tascher, G; Burban, A; Camus, S; Plumel, M; Chanon, S; Guevel, Le R; Shevchenko, V; Dorsselaer, Van A; Lefai, E; Guguen-Guillouzo, C; Bertile, F In-Depth Proteome Analysis Highlights HepaRG Cells as a Versatile Cell System Surrogate for Primary Human Hepatocytes Article de journal Cells, 8 (2), p. 192, 2019, (2012/20). @article{629, title = {In-Depth Proteome Analysis Highlights HepaRG Cells as a Versatile Cell System Surrogate for Primary Human Hepatocytes}, author = {G Tascher and A Burban and S Camus and M Plumel and S Chanon and R Le Guevel and V Shevchenko and A Van Dorsselaer and E Lefai and C Guguen-Guillouzo and F Bertile}, doi = {10.3390/cells8020192}, year = {2019}, date = {2019-02-21}, journal = {Cells}, volume = {8}, number = {2}, pages = {192}, note = {2012/20}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Beck, A; D'Atri, V; Ehkirch, A; Fekete, S; Hernandez-Alba, O; Gahoual, R; Leize-Wagner, E; François, Y; Guillarme, D; Cianférani, S Cutting-edge multi-level analytical and structural characterization of antibody-drug conjugates: present and future Article de journal Expert Rev Proteomics., 16 (4), p. 337-362., 2019, (review). @article{633, title = {Cutting-edge multi-level analytical and structural characterization of antibody-drug conjugates: present and future}, author = {A Beck and V D'Atri and A Ehkirch and S Fekete and O Hernandez-Alba and R Gahoual and E Leize-Wagner and Y François and D Guillarme and S Cianférani}, doi = {10.1080/14789450.2019.1578215}, year = {2019}, date = {2019-02-18}, journal = {Expert Rev Proteomics.}, volume = {16}, number = {4}, pages = {337-362.}, note = {review}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Locard-Paulet, M; Parra, J; Albigot, R; Mouton-Barbosa, E; Bardi, L; Burlet-Schiltz, O; Marcoux, J VisioProt-MS: interactive 2D maps from intact protein mass spectrometry Article de journal Bioinformatics, 35 (4), p. 679–681, 2019. @article{pmid30084957, title = {VisioProt-MS: interactive 2D maps from intact protein mass spectrometry}, author = {M Locard-Paulet and J Parra and R Albigot and E Mouton-Barbosa and L Bardi and O Burlet-Schiltz and J Marcoux}, doi = {10.1093/bioinformatics/bty680}, year = {2019}, date = {2019-02-15}, journal = {Bioinformatics}, volume = {35}, number = {4}, pages = {679--681}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Strassel, C; Magiera, MM.; Dupuis, A; Batzenschlager, M; Hovasse, A; Pleines, I; Guéguen, P; Eckly, A; Moog, S; Mallo, L; Kimmerlin, Q; Chappaz, S; Strub, JM.; Kathiresan, N; de la Salle, H; Dorsselaer, Van A; Ferec, C; Py, JY.; Gachet, C; Schaeffer-Reiss, C; Kile, BT.; Janke, C; Lanza, F An essential role for α4A-tubulin in platelet biogenesis Article de journal Life Sci Alliance, 2 (1), p. pii: e201900309., 2019. @article{637, title = {An essential role for α4A-tubulin in platelet biogenesis}, author = {C Strassel and MM. Magiera and A Dupuis and M Batzenschlager and A Hovasse and I Pleines and P Guéguen and A Eckly and S Moog and L Mallo and Q Kimmerlin and S Chappaz and JM. Strub and N Kathiresan and H de la Salle and A Van Dorsselaer and C Ferec and JY. Py and C Gachet and C Schaeffer-Reiss and BT. Kile and C Janke and F Lanza}, doi = {10.26508/lsa.201900309}, year = {2019}, date = {2019-02-13}, journal = {Life Sci Alliance}, volume = {2}, number = {1}, pages = {pii: e201900309.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Hyenne, V; Ghoroghi, S; Collot, M; Bons, J; Follain, G; Harlepp, S; Mary, B; Bauer, J; Mercier, L; Busnelli, I; Lefebvre, O; Fekonja, N; Garcia-Leon, MJ.; Machado, P; Delalande, F; López, AA.; Silva, SG.; Verweij, FJ.; van Niel, G; Djouad, F; Peinado, H; Carapito, C; Klymchenko, AS.; Goetz, JG. Studying the Fate of Tumor Extracellular Vesicles at High Spatiotemporal Resolution Using the Zebrafish Embryo Article de journal Dev Cell., 48 (4), p. 554-572.e7, 2019. @article{635, title = {Studying the Fate of Tumor Extracellular Vesicles at High Spatiotemporal Resolution Using the Zebrafish Embryo}, author = {V Hyenne and S Ghoroghi and M Collot and J Bons and G Follain and S Harlepp and B Mary and J Bauer and L Mercier and I Busnelli and O Lefebvre and N Fekonja and MJ. Garcia-Leon and P Machado and F Delalande and AA. López and SG. Silva and FJ. Verweij and G van Niel and F Djouad and H Peinado and C Carapito and AS. Klymchenko and JG. Goetz}, doi = {10.1016/j.devcel.2019.01.014}, year = {2019}, date = {2019-02-07}, journal = {Dev Cell.}, volume = {48}, number = {4}, pages = {554-572.e7}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Menneteau, T; Fabre, B; Garrigues, L; Stella, A; Zivkovic, D; Roux-Dalvai, F; Mouton-Barbosa, E; Beau, M; Renoud, M L; Amalric, F; Sensébé, L; Gonzalez-de-Peredo, A; Ader, I; Burlet-Schiltz, O; Bousquet, M P Mass Spectrometry-based Absolute Quantification of 20S Proteasome Status for Controlled Ex-vivo Expansion of Ħuman Adipose-derived Mesenchymal Stromal/Stem Cells Article de journal Mol. Cell Proteomics, 18 (4), p. 744–759, 2019. @article{pmid30700495, title = {Mass Spectrometry-based Absolute Quantification of 20S Proteasome Status for Controlled Ex-vivo Expansion of Ħuman Adipose-derived Mesenchymal Stromal/Stem Cells}, author = {T Menneteau and B Fabre and L Garrigues and A Stella and D Zivkovic and F Roux-Dalvai and E Mouton-Barbosa and M Beau and M L Renoud and F Amalric and L Sensébé and A Gonzalez-de-Peredo and I Ader and O Burlet-Schiltz and M P Bousquet}, doi = {10.1074/mcp.RA118.000958}, year = {2019}, date = {2019-01-30}, journal = {Mol. Cell Proteomics}, volume = {18}, number = {4}, pages = {744--759}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fornecker, LM.; Muller, L; Bertrand, F; Paul, N; Pichot, A; Herbrecht, R; Chenard, MP.; Mauvieux, L; Vallat, L; Bahram, S; Cianférani, S; Carapito, R; Carapito, C Multi-omics dataset to decipher the complexity of drug resistance in diffuse large B-cell lymphoma Article de journal Sci Rep., 9 (1), p. 895, 2019. @article{628, title = {Multi-omics dataset to decipher the complexity of drug resistance in diffuse large B-cell lymphoma}, author = {LM. Fornecker and L Muller and F Bertrand and N Paul and A Pichot and R Herbrecht and MP. Chenard and L Mauvieux and L Vallat and S Bahram and S Cianférani and R Carapito and C Carapito}, doi = {10.1038/s41598-018-37273-4}, year = {2019}, date = {2019-01-29}, journal = {Sci Rep.}, volume = {9}, number = {1}, pages = {895}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Aniort, J; Stella, A; Philipponnet, C; Poyet, A; Polge, C; Claustre, A; Combaret, L; B?chet, D; Attaix, D; Boisgard, S; Filaire, M; Rosset, E; Burlet-Schiltz, O; Heng, A E; Taillandier, D Muscle wasting in patients with end-stage renal disease or early-stage lung cancer: common mechanisms at work Article de journal J Cachexia Sarcopenia Muscle, 10 (2), p. 323–337, 2019. @article{pmid30697967, title = {Muscle wasting in patients with end-stage renal disease or early-stage lung cancer: common mechanisms at work}, author = {J Aniort and A Stella and C Philipponnet and A Poyet and C Polge and A Claustre and L Combaret and D B?chet and D Attaix and S Boisgard and M Filaire and E Rosset and O Burlet-Schiltz and A E Heng and D Taillandier}, doi = {0.1002/jcsm.12376}, year = {2019}, date = {2019-01-29}, journal = {J Cachexia Sarcopenia Muscle}, volume = {10}, number = {2}, pages = {323--337}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Groysbeck, N; Stoessel, A; Donzeau, M; da Silva, EC.; Lehmann, M; Strub, JM.; Cianferani, S; Dembélé, K; Zuber, G Synthesis and biological evaluation of 2.4 nm thiolate-protected gold nanoparticles conjugated to Cetuximab for targeting glioblastoma cancer cells via the EGFR Article de journal Nanotechnology, 30 (18), p. 184005., 2019. @article{634, title = {Synthesis and biological evaluation of 2.4 nm thiolate-protected gold nanoparticles conjugated to Cetuximab for targeting glioblastoma cancer cells via the EGFR}, author = {N Groysbeck and A Stoessel and M Donzeau and EC. da Silva and M Lehmann and JM. Strub and S Cianferani and K Dembélé and G Zuber}, doi = { 10.1088/1361-6528/aaff0a}, year = {2019}, date = {2019-01-16}, journal = {Nanotechnology}, volume = {30}, number = {18}, pages = {184005.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rochel, N; Krucker, C; Coutos-Thévenot, L; Osz, J; Zhang, R; Guyon, E; Zita, W; Vanthong, S; Hernandez-Alba, O; Bourguet, M; Badawy, Al K; Dufour, F; Peluso-Iltis, C; Heckler-Beji, S; Dejaegere, A; Kamoun, A; de Reyniès, A; Neuzillet, Y; Rebouissou, S; Béraud, C; Lang, H; Massfelder, T; Allory, Y; Cianférani, S; Stote, R H; Radvanyi, F; Bernard-Pierrot, I Recurrent activating mutations of PPARγ associated with luminal bladder tumors Article de journal Nature Communications, 10 (1), p. 253, 2019. @article{627, title = {Recurrent activating mutations of PPARγ associated with luminal bladder tumors}, author = {N Rochel and C Krucker and L Coutos-Thévenot and J Osz and R Zhang and E Guyon and W Zita and S Vanthong and O Hernandez-Alba and M Bourguet and K Al Badawy and F Dufour and C Peluso-Iltis and S Heckler-Beji and A Dejaegere and A Kamoun and A de Reyniès and Y Neuzillet and S Rebouissou and C Béraud and H Lang and T Massfelder and Y Allory and S Cianférani and R H Stote and F Radvanyi and I Bernard-Pierrot}, doi = {10.1038/s41467-018-08157-y}, year = {2019}, date = {2019-01-16}, journal = {Nature Communications}, volume = {10}, number = {1}, pages = {253}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Niazi, AK.; Bariat, L; Riondet, C; Carapito, C; Mhamdi, A; Noctor, G; Reichheld, JP. Cytosolic Isocitrate Dehydrogenase from Arabidopsis thaliana Is Regulated by Glutathionylation Article de journal Antioxidants (Basel), 8 (1), p. pii: E16., 2019. @article{636, title = {Cytosolic Isocitrate Dehydrogenase from Arabidopsis thaliana Is Regulated by Glutathionylation}, author = {AK. Niazi and L Bariat and C Riondet and C Carapito and A Mhamdi and G Noctor and JP. Reichheld}, doi = {10.3390/antiox8010016}, year = {2019}, date = {2019-01-08}, journal = {Antioxidants (Basel)}, volume = {8}, number = {1}, pages = {pii: E16.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2018 |
Boeuf, A; Schnell, G; Bernard, Q; Kern, A; Westermann, B; Ehret-Sabatier, L; Grillon, A; Schramm, F; Jaulhac, B; Boulanger, N Dissociating effect of salivary gland extract from Ixodes ricinus on human fibroblasts: Potential impact on Borrelia transmission Article de journal Ticks Tick Borne Dis., 10 (2), p. 433–441, 2018, (2012/29). @article{626b, title = {Dissociating effect of salivary gland extract from Ixodes ricinus on human fibroblasts: Potential impact on Borrelia transmission}, author = {A Boeuf and G Schnell and Q Bernard and A Kern and B Westermann and L Ehret-Sabatier and A Grillon and F Schramm and B Jaulhac and N Boulanger}, doi = {10.1016/j.ttbdis.2018.12.005}, year = {2018}, date = {2018-12-23}, journal = {Ticks Tick Borne Dis.}, volume = {10}, number = {2}, pages = {433–441}, note = {2012/29}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tascher, G; Gerbaix, M; Maes, P; Chazarin, B; Ghislin, S; Antropova, E; Vassilieva, G; Ouzren-Zarhloul, N; Gauquelin-Koch, G; Vico, L; Frippiat, JP.; Bertile, F Analysis of femurs from mice embarked on board BION-M1 biosatellite reveals a decrease in immune cell development, including B cells, after 1 wk of recovery on Earth Article de journal FASEB J., 33 (3), 2018. @article{625b, title = {Analysis of femurs from mice embarked on board BION-M1 biosatellite reveals a decrease in immune cell development, including B cells, after 1 wk of recovery on Earth}, author = {G Tascher and M Gerbaix and P Maes and B Chazarin and S Ghislin and E Antropova and G Vassilieva and N Ouzren-Zarhloul and G Gauquelin-Koch and L Vico and JP. Frippiat and F Bertile}, doi = {10.1096/fj.201801463R}, year = {2018}, date = {2018-12-06}, journal = {FASEB J.}, volume = {33}, number = {3}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Jonckheere, Julie; Deloulme, Jean-Christophe; Dall'Igna, Gaëlle; Chauliac, Nicolas; Pelluet, Albane; Nguon, Anne-Sophie; Lentini, Celia; Brocard, Jacques; Denarier, Eric; Brugière, Sabine; Couté, Yohann; Heinrich, Christophe; Porcher, Christophe; Holtzmann, Jérôme; Andrieux, Annie; Suaud-Chagny, Marie-Françoise; Gory-Fauré, Sylvie Short- and long-term efficacy of electroconvulsive stimulation in animal models of depression: The essential role of neuronal survival Article de journal Brain Stimulation, 11 (6), p. 1336–1347, 2018, ISSN: 1876-4754. @article{jonckheere_short-_2018, title = {Short- and long-term efficacy of electroconvulsive stimulation in animal models of depression: The essential role of neuronal survival}, author = {Julie Jonckheere and Jean-Christophe Deloulme and Gaëlle Dall'Igna and Nicolas Chauliac and Albane Pelluet and Anne-Sophie Nguon and Celia Lentini and Jacques Brocard and Eric Denarier and Sabine Brugière and Yohann Couté and Christophe Heinrich and Christophe Porcher and Jérôme Holtzmann and Annie Andrieux and Marie-Françoise Suaud-Chagny and Sylvie Gory-Fauré}, doi = {10.1016/j.brs.2018.08.001}, issn = {1876-4754}, year = {2018}, date = {2018-12-01}, journal = {Brain Stimulation}, volume = {11}, number = {6}, pages = {1336--1347}, abstract = {BACKGROUND: Severe and medication-resistant psychiatric diseases, such as major depressive disorder, bipolar disorder or schizophrenia, can be effectively and rapidly treated by electroconvulsive therapy (ECT). Despite extensive long-standing clinical use, the neurobiological mechanisms underlying the curative action of ECT remain incompletely understood. OBJECTIVE: Unravel biological basis of electroconvulsive stimulation (ECS) efficacy, the animal equivalent of ECT. METHODS: Using MAP6 KO mouse, a genetic model that constitutively exhibits features relevant to some aspects of depression; we analyzed the behavioral and biological consequences of ECS treatment alone (10 stimulations over a 2-week period) and associated with a continuation protocol (2 stimulations per week for 5 weeks). RESULTS: ECS treatment had a beneficial effect on constitutive behavioral defects. We showed that behavioral improvement is associated with a strong increase in the survival and integration of neurons born before ECS treatment. Retroviral infection revealed the larger number of integrated neurons to exhibit increased dendritic complexity and spine density, as well as remodeled synapses. Furthermore, our results show that ECS triggers a cortical increase in synaptogenesis. A sustained newborn neuron survival rate, induced by ECS treatment, is associated with the behavioral improvement, but relapse occurred 40 days after completing the ECS treatment. However, a 5-week continuation protocol following the initial ECS treatment led to persistent improvement of behavior correlated with sustained rate survival of newborn neurons. CONCLUSION: Altogether, these results reveal that increased synaptic connectivity and extended neuronal survival are key to the short and long-term efficacy of ECS.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BACKGROUND: Severe and medication-resistant psychiatric diseases, such as major depressive disorder, bipolar disorder or schizophrenia, can be effectively and rapidly treated by electroconvulsive therapy (ECT). Despite extensive long-standing clinical use, the neurobiological mechanisms underlying the curative action of ECT remain incompletely understood. OBJECTIVE: Unravel biological basis of electroconvulsive stimulation (ECS) efficacy, the animal equivalent of ECT. METHODS: Using MAP6 KO mouse, a genetic model that constitutively exhibits features relevant to some aspects of depression; we analyzed the behavioral and biological consequences of ECS treatment alone (10 stimulations over a 2-week period) and associated with a continuation protocol (2 stimulations per week for 5 weeks). RESULTS: ECS treatment had a beneficial effect on constitutive behavioral defects. We showed that behavioral improvement is associated with a strong increase in the survival and integration of neurons born before ECS treatment. Retroviral infection revealed the larger number of integrated neurons to exhibit increased dendritic complexity and spine density, as well as remodeled synapses. Furthermore, our results show that ECS triggers a cortical increase in synaptogenesis. A sustained newborn neuron survival rate, induced by ECS treatment, is associated with the behavioral improvement, but relapse occurred 40 days after completing the ECS treatment. However, a 5-week continuation protocol following the initial ECS treatment led to persistent improvement of behavior correlated with sustained rate survival of newborn neurons. CONCLUSION: Altogether, these results reveal that increased synaptic connectivity and extended neuronal survival are key to the short and long-term efficacy of ECS. |
Albaret, Marie Alexandra; Vermot-Desroches, Claudine; Paré, Arnaud; Roca-Martinez, Jean-Xavier; Malet, Lucie; Esseily, Jad; Gerossier, Laetitia; Brière, Johan; Pion, Nathalie; Marcel, Virginie; Catez, Frédéric; Souza, Geneviève De; Vuillermoz, Boris; Doerflinger, Franck; Lavocat, Emilie; Subiger, Olivier; Rousset, Carine; Bresson, Corinne; Mandon, Elodie; Jawhari, Anass; Falson, Pierre; Jasmin, Mélissa; Couté, Yohann; Mertani, Hichem-Claude; Saintigny, Pierre; Diaz, Jean-Jacques Externalized Keratin 8: A Target at the Interface of Microenvironment and Intracellular Signaling in Colorectal Cancer Cells Article de journal Cancers, 10 (11), 2018, ISSN: 2072-6694. @article{albaret_externalized_2018, title = {Externalized Keratin 8: A Target at the Interface of Microenvironment and Intracellular Signaling in Colorectal Cancer Cells}, author = {Marie Alexandra Albaret and Claudine Vermot-Desroches and Arnaud Paré and Jean-Xavier Roca-Martinez and Lucie Malet and Jad Esseily and Laetitia Gerossier and Johan Brière and Nathalie Pion and Virginie Marcel and Frédéric Catez and Geneviève De Souza and Boris Vuillermoz and Franck Doerflinger and Emilie Lavocat and Olivier Subiger and Carine Rousset and Corinne Bresson and Elodie Mandon and Anass Jawhari and Pierre Falson and Mélissa Jasmin and Yohann Couté and Hichem-Claude Mertani and Pierre Saintigny and Jean-Jacques Diaz}, doi = {10.3390/cancers10110452}, issn = {2072-6694}, year = {2018}, date = {2018-11-01}, journal = {Cancers}, volume = {10}, number = {11}, abstract = {Accumulating evidence supports the remarkable presence at the membrane surface of cancer cells of proteins, which are normally expressed in the intracellular compartment. Although these proteins, referred to as externalized proteins, represent a highly promising source of accessible and druggable targets for cancer therapy, the mechanisms via which they impact cancer biology remain largely unexplored. The aim of this study was to expose an externalized form of cytokeratin 8 (eK8) as a key player of colorectal tumorigenesis and characterize its mode of action. To achieve this, we generated a unique antagonist monoclonal antibody (D-A10 MAb) targeting an eight-amino-acid-long domain of eK8, which enabled us to ascertain the pro-tumoral activity of eK8 in both KRAS-mutant and wild-type colorectal cancers (CRC). We showed that this pro-tumoral activity involves a bidirectional eK8-dependent control of caspase-mediated apoptosis in vivo and of the plasminogen-induced invasion process in cellulo. Furthermore, we demonstrated that eK8 is anchored at the plasma membrane supporting this dual function. We, therefore, identified eK8 as an innovative therapeutic target in CRC and provided a unique MAb targeting eK8 that displays anti-neoplastic activities that could be useful to treat CRC, including those harboring KRAS mutations.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Accumulating evidence supports the remarkable presence at the membrane surface of cancer cells of proteins, which are normally expressed in the intracellular compartment. Although these proteins, referred to as externalized proteins, represent a highly promising source of accessible and druggable targets for cancer therapy, the mechanisms via which they impact cancer biology remain largely unexplored. The aim of this study was to expose an externalized form of cytokeratin 8 (eK8) as a key player of colorectal tumorigenesis and characterize its mode of action. To achieve this, we generated a unique antagonist monoclonal antibody (D-A10 MAb) targeting an eight-amino-acid-long domain of eK8, which enabled us to ascertain the pro-tumoral activity of eK8 in both KRAS-mutant and wild-type colorectal cancers (CRC). We showed that this pro-tumoral activity involves a bidirectional eK8-dependent control of caspase-mediated apoptosis in vivo and of the plasminogen-induced invasion process in cellulo. Furthermore, we demonstrated that eK8 is anchored at the plasma membrane supporting this dual function. We, therefore, identified eK8 as an innovative therapeutic target in CRC and provided a unique MAb targeting eK8 that displays anti-neoplastic activities that could be useful to treat CRC, including those harboring KRAS mutations. |
Hahn, Friedrich; Hutterer, Corina; Henry, Christophe; Hamilton, Stuart T; Strojan, Hanife; Kraut, Alexandra; Schulte, Ulrike; Schütz, Martin; Kohrt, Stephan; Wangen, Christina; Pfizer, José; Couté, Yohann; Rawlinson, William D; Strobl, Stefan; Marschall, Manfred Novel cytomegalovirus-inhibitory compounds of the class pyrrolopyridines show a complex pattern of target binding that suggests an unusual mechanism of antiviral activity Article de journal Antiviral Research, 159 , p. 84–94, 2018, ISSN: 1872-9096. @article{hahn_novel_2018, title = {Novel cytomegalovirus-inhibitory compounds of the class pyrrolopyridines show a complex pattern of target binding that suggests an unusual mechanism of antiviral activity}, author = {Friedrich Hahn and Corina Hutterer and Christophe Henry and Stuart T Hamilton and Hanife Strojan and Alexandra Kraut and Ulrike Schulte and Martin Schütz and Stephan Kohrt and Christina Wangen and José Pfizer and Yohann Couté and William D Rawlinson and Stefan Strobl and Manfred Marschall}, doi = {10.1016/j.antiviral.2018.09.012}, issn = {1872-9096}, year = {2018}, date = {2018-11-01}, journal = {Antiviral Research}, volume = {159}, pages = {84--94}, abstract = {Human cytomegalovirus (HCMV) is a major human pathogen with seropositivity rates in the adult population ranging between 40% and 95%. HCMV infection is associated with severe pathology, such as life-threatening courses of infection in immunocompromised individuals and neonates. Current standard therapy with valganciclovir has the disadvantage of adverse side effects and viral drug resistance. A novel anti-HCMV drug, letermovir, has been approved recently, so that improved therapy options are available. Nevertheless, even more so far unexploited classes of compounds and molecular modes of action will be required for a next generation of antiherpesviral treatment strategies. In this study, we focused on the analysis of the antiviral potency of a novel class of compounds, i.e. pyrrolopyridine analogs, and identified both hit compounds and their target protein candidates. In essence, we provide novel evidence as follows: (i) screening hit SC88941 is highly active in inhibiting HCMV replication in primary human fibroblasts with an EC50 value of 0.20 ± 0.01 μM in the absence of cytotoxicity, (ii) inhibition occurs at the early-late stage of viral protein production and shows reinforcing effects upon LMV cotreatment, (iii) among the viruses analyzed, antiviral activity was most pronounced against β-herpesviruses (HCMV, HHV-6A) and intermediate against adenovirus (HAdV-2), (iv) induction of SC88941 resistance was not detectable, thus differed from the induction of ganciclovir resistance, (v) a linker-coupled model compound was used for mass spectrometry-based target identification, thus yielding several drug-binding target proteins and (vi) a first confocal imaging approach used for addressing intracellular effects of SC88941 indicated qualitative and quantitative alteration of viral protein expression and localization. Thus, our findings suggest a multifaceted pattern of compound-target binding in connection with an unusual mode of action, opening up further opportunities of antiviral drug development.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Human cytomegalovirus (HCMV) is a major human pathogen with seropositivity rates in the adult population ranging between 40% and 95%. HCMV infection is associated with severe pathology, such as life-threatening courses of infection in immunocompromised individuals and neonates. Current standard therapy with valganciclovir has the disadvantage of adverse side effects and viral drug resistance. A novel anti-HCMV drug, letermovir, has been approved recently, so that improved therapy options are available. Nevertheless, even more so far unexploited classes of compounds and molecular modes of action will be required for a next generation of antiherpesviral treatment strategies. In this study, we focused on the analysis of the antiviral potency of a novel class of compounds, i.e. pyrrolopyridine analogs, and identified both hit compounds and their target protein candidates. In essence, we provide novel evidence as follows: (i) screening hit SC88941 is highly active in inhibiting HCMV replication in primary human fibroblasts with an EC50 value of 0.20 ± 0.01 μM in the absence of cytotoxicity, (ii) inhibition occurs at the early-late stage of viral protein production and shows reinforcing effects upon LMV cotreatment, (iii) among the viruses analyzed, antiviral activity was most pronounced against β-herpesviruses (HCMV, HHV-6A) and intermediate against adenovirus (HAdV-2), (iv) induction of SC88941 resistance was not detectable, thus differed from the induction of ganciclovir resistance, (v) a linker-coupled model compound was used for mass spectrometry-based target identification, thus yielding several drug-binding target proteins and (vi) a first confocal imaging approach used for addressing intracellular effects of SC88941 indicated qualitative and quantitative alteration of viral protein expression and localization. Thus, our findings suggest a multifaceted pattern of compound-target binding in connection with an unusual mode of action, opening up further opportunities of antiviral drug development. |
Borges, Hélène; Guibert, Romain; Permiakova, Olga; Burger, Thomas Distinguishing between Spectral Clustering and Cluster Analysis of Mass Spectra Article de journal Journal of Proteome Research, 2018, ISSN: 1535-3907. @article{borges_distinguishing_2018, title = {Distinguishing between Spectral Clustering and Cluster Analysis of Mass Spectra}, author = {Hélène Borges and Romain Guibert and Olga Permiakova and Thomas Burger}, doi = {10.1021/acs.jproteome.8b00516}, issn = {1535-3907}, year = {2018}, date = {2018-11-01}, journal = {Journal of Proteome Research}, abstract = {The term "spectral clustering" is sometimes used to refer to the clustering of mass spectrometry data. However, it also classically refers to a family of popular clustering algorithms. To avoid confusion, a more specific term could advantageously be coined.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The term "spectral clustering" is sometimes used to refer to the clustering of mass spectrometry data. However, it also classically refers to a family of popular clustering algorithms. To avoid confusion, a more specific term could advantageously be coined. |
Dominguez-Medina, Sergio; Fostner, Shawn; Defoort, Martial; Sansa, Marc; Stark, Ann-Kathrin; Halim, Mohammad Abdul; Vernhes, Emeline; Gely, Marc; Jourdan, Guillaume; Alava, Thomas; Boulanger, Pascale; Masselon, Christophe; Hentz, Sébastien Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators Article de journal Science (New York, N.Y.), 362 (6417), p. 918–922, 2018, ISSN: 1095-9203. @article{dominguez-medina_neutral_2018, title = {Neutral mass spectrometry of virus capsids above 100 megadaltons with nanomechanical resonators}, author = {Sergio Dominguez-Medina and Shawn Fostner and Martial Defoort and Marc Sansa and Ann-Kathrin Stark and Mohammad Abdul Halim and Emeline Vernhes and Marc Gely and Guillaume Jourdan and Thomas Alava and Pascale Boulanger and Christophe Masselon and Sébastien Hentz}, doi = {10.1126/science.aat6457}, issn = {1095-9203}, year = {2018}, date = {2018-11-01}, journal = {Science (New York, N.Y.)}, volume = {362}, number = {6417}, pages = {918--922}, abstract = {Measurement of the mass of particles in the mega- to gigadalton range is challenging with conventional mass spectrometry. Although this mass range appears optimal for nanomechanical resonators, nanomechanical mass spectrometers often suffer from prohibitive sample loss, extended analysis time, or inadequate resolution. We report on a system architecture combining nebulization of the analytes from solution, their efficient transfer and focusing without relying on electromagnetic fields, and the mass measurements of individual particles using nanomechanical resonator arrays. This system determined the mass distribution of textasciitilde30-megadalton polystyrene nanoparticles with high detection efficiency and effectively performed molecular mass measurements of empty or DNA-filled bacteriophage T5 capsids with masses up to 105 megadaltons using less than 1 picomole of sample and with an instrument resolution above 100.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Measurement of the mass of particles in the mega- to gigadalton range is challenging with conventional mass spectrometry. Although this mass range appears optimal for nanomechanical resonators, nanomechanical mass spectrometers often suffer from prohibitive sample loss, extended analysis time, or inadequate resolution. We report on a system architecture combining nebulization of the analytes from solution, their efficient transfer and focusing without relying on electromagnetic fields, and the mass measurements of individual particles using nanomechanical resonator arrays. This system determined the mass distribution of textasciitilde30-megadalton polystyrene nanoparticles with high detection efficiency and effectively performed molecular mass measurements of empty or DNA-filled bacteriophage T5 capsids with masses up to 105 megadaltons using less than 1 picomole of sample and with an instrument resolution above 100. |
Do, Le Duy; Gupton, Stéphanie L; Tanji, Kunikazu; Bastien, Joubert; Brugière, Sabine; Couté, Yohann; Quadrio, Isabelle; Rogemond, Véronique; Fabien, Nicole; Desestret, Virginie; Honnorat, Jérôme TRIM9 and TRIM67 Are New Targets in Paraneoplastic Cerebellar Degeneration Article de journal Cerebellum (London, England), 2018, ISSN: 1473-4230. @article{do_trim9_2018, title = {TRIM9 and TRIM67 Are New Targets in Paraneoplastic Cerebellar Degeneration}, author = {Le Duy Do and Stéphanie L Gupton and Kunikazu Tanji and Joubert Bastien and Sabine Brugière and Yohann Couté and Isabelle Quadrio and Véronique Rogemond and Nicole Fabien and Virginie Desestret and Jérôme Honnorat}, doi = {10.1007/s12311-018-0987-5}, issn = {1473-4230}, year = {2018}, date = {2018-10-01}, journal = {Cerebellum (London, England)}, abstract = {To describe autoantibodies (Abs) against tripartite motif-containing (TRIM) protein 9 and 67 in two patients with paraneoplastic cerebellar degeneration (PCD) associated with lung adenocarcinoma. Abs were characterized using immunohistochemistry, Western blotting, cultures of murine cortical, and hippocampal neurons, immunoprecipitation, mass spectrometry, knockout mice for Trim9 and 67, and cell-based assay. Control samples included sera from 63 patients with small cell lung cancer without any paraneoplastic neurological syndrome, 36 patients with lung adenocarcinoma and PNS, CSF from 100 patients with autoimmune encephalitis, and CSF from 165 patients with neurodegenerative diseases. We found Abs targeting TRIM9 and TRIM67 at high concentration in the serum and the cerebrospinal fluid (CSF) of a 78-year-old woman and a 65-year-old man. Both developed subacute severe cerebellar ataxia. Brain magnetic resonance imaging found no abnormality and no cerebellar atrophy. Both had CSF inflammation with mild pleiocytosis and a few oligoclonal bands. We identified a pulmonary adenocarcinoma, confirming the paraneoplastic neurological syndrome in both patients. They received immunomodulatory and cancer treatments without improvement of cerebellar ataxia, even though both were in remission of their cancer (for more than 10 years in one patient). Anti-TRIM9 and anti-TRIM67 Abs were specific to these two patients. All control serum and CSF samples tested were negative for anti-TRIM9 and 67. Anti-TRIM9 and anti-TRIM67 Abs appeared to be specific biomarkers of PCD and should be added to the panel of antigens tested when this is suspected.}, keywords = {}, pubstate = {published}, tppubtype = {article} } To describe autoantibodies (Abs) against tripartite motif-containing (TRIM) protein 9 and 67 in two patients with paraneoplastic cerebellar degeneration (PCD) associated with lung adenocarcinoma. Abs were characterized using immunohistochemistry, Western blotting, cultures of murine cortical, and hippocampal neurons, immunoprecipitation, mass spectrometry, knockout mice for Trim9 and 67, and cell-based assay. Control samples included sera from 63 patients with small cell lung cancer without any paraneoplastic neurological syndrome, 36 patients with lung adenocarcinoma and PNS, CSF from 100 patients with autoimmune encephalitis, and CSF from 165 patients with neurodegenerative diseases. We found Abs targeting TRIM9 and TRIM67 at high concentration in the serum and the cerebrospinal fluid (CSF) of a 78-year-old woman and a 65-year-old man. Both developed subacute severe cerebellar ataxia. Brain magnetic resonance imaging found no abnormality and no cerebellar atrophy. Both had CSF inflammation with mild pleiocytosis and a few oligoclonal bands. We identified a pulmonary adenocarcinoma, confirming the paraneoplastic neurological syndrome in both patients. They received immunomodulatory and cancer treatments without improvement of cerebellar ataxia, even though both were in remission of their cancer (for more than 10 years in one patient). Anti-TRIM9 and anti-TRIM67 Abs were specific to these two patients. All control serum and CSF samples tested were negative for anti-TRIM9 and 67. Anti-TRIM9 and anti-TRIM67 Abs appeared to be specific biomarkers of PCD and should be added to the panel of antigens tested when this is suspected. |
Völz, Ronny; Kim, Soon-Kap; Mi, Jianing; Mariappan, Kiruthiga G; Guo, Xiujie; Bigeard, Jean; Alejandro, Santiago; Pflieger, Delphine; Rayapuram, Naganand; Al-Babili, Salim; Hirt, Heribert The Trihelix transcription factor GT2-like 1 (GTL1) promotes salicylic acid metabolism, and regulates bacterial-triggered immunity Article de journal PLoS genetics, 14 (10), p. e1007708, 2018, ISSN: 1553-7404. @article{volz_trihelix_2018, title = {The Trihelix transcription factor GT2-like 1 (GTL1) promotes salicylic acid metabolism, and regulates bacterial-triggered immunity}, author = {Ronny Völz and Soon-Kap Kim and Jianing Mi and Kiruthiga G Mariappan and Xiujie Guo and Jean Bigeard and Santiago Alejandro and Delphine Pflieger and Naganand Rayapuram and Salim Al-Babili and Heribert Hirt}, doi = {10.1371/journal.pgen.1007708}, issn = {1553-7404}, year = {2018}, date = {2018-10-01}, journal = {PLoS genetics}, volume = {14}, number = {10}, pages = {e1007708}, abstract = {The Trihelix Transcription factor GT2-like 1 (GTL1) was previously shown to be a key regulator of ploidy-dependent trichome growth and drought tolerance. Here, we report that GTL1 plays an important role in coordinating plant immunity. We show that gtl1 mutants are compromised in the regulation of basal immunity, microbial pattern-triggered immunity (PTI) and effector-triggered RIN4-mediated immunity. Transcriptome analysis revealed that GTL1 positively regulates defense genes and inhibits factors that mediate growth and development. By performing hormonal measurements and chromatin-immunoprecipitation studies, we found GTL1 to coordinate genes involved in salicylic acid metabolism, transport and response. Interaction studies and comparative transcriptomics to known data sets revealed that GTL1 is part of the MPK4 pathway and regulates oppositely the expression of differentially expressed genes in mpk4 plants. We introduced the gtl1 mutation in the mpk4 mutant and thereby partially suppressed its dwarfism and the high resistance against a bacterial invader. Our data show that GTL1 is part of the MPK4 pathway and acts as a positive regulator of bacterial-triggered immunity and SA homeostasis.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The Trihelix Transcription factor GT2-like 1 (GTL1) was previously shown to be a key regulator of ploidy-dependent trichome growth and drought tolerance. Here, we report that GTL1 plays an important role in coordinating plant immunity. We show that gtl1 mutants are compromised in the regulation of basal immunity, microbial pattern-triggered immunity (PTI) and effector-triggered RIN4-mediated immunity. Transcriptome analysis revealed that GTL1 positively regulates defense genes and inhibits factors that mediate growth and development. By performing hormonal measurements and chromatin-immunoprecipitation studies, we found GTL1 to coordinate genes involved in salicylic acid metabolism, transport and response. Interaction studies and comparative transcriptomics to known data sets revealed that GTL1 is part of the MPK4 pathway and regulates oppositely the expression of differentially expressed genes in mpk4 plants. We introduced the gtl1 mutation in the mpk4 mutant and thereby partially suppressed its dwarfism and the high resistance against a bacterial invader. Our data show that GTL1 is part of the MPK4 pathway and acts as a positive regulator of bacterial-triggered immunity and SA homeostasis. |
Gervais, Virginie; Mari, Isabelle Muller Pierre-Olivier; Mourcet, Amandine; Movellan, Kumar Tekwani; Ramos, Pascal; Marcoux, Julien; Guillet, Valérie; Javaid, Sumaira; Burlet-Schiltz, Odile; Czaplicki, Georges; Milon, Alain; Giglia-Mari, Giuseppina Small molecule-based targeting of TTD-A dimerization to control TFIIH transcriptional activity represents a potential strategy for anticancer therapy Article de journal J Biol Chem., 293 (39), p. 14974-14988, 2018. @article{Gervais2018, title = {Small molecule-based targeting of TTD-A dimerization to control TFIIH transcriptional activity represents a potential strategy for anticancer therapy}, author = {Virginie Gervais and Isabelle Muller Pierre-Olivier Mari and Amandine Mourcet and Kumar Tekwani Movellan and Pascal Ramos and Julien Marcoux and Valérie Guillet and Sumaira Javaid and Odile Burlet-Schiltz and Georges Czaplicki and Alain Milon and Giuseppina Giglia-Mari}, doi = {10.1074/jbc.RA118.003444}, year = {2018}, date = {2018-09-28}, journal = {J Biol Chem.}, volume = {293}, number = {39}, pages = {14974-14988}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Shiota, Hitoshi; Barral, Sophie; Buchou, Thierry; Tan, Minjia; Couté, Yohann; Charbonnier, Guillaume; Reynoird, Nicolas; Boussouar, Fayçal; Gérard, Matthieu; Zhu, Mingrui; Bargier, Lisa; Puthier, Denis; Chuffart, Florent; Bourova-Flin, Ekaterina; Picaud, Sarah; Filippakopoulos, Panagis; Goudarzi, Afsaneh; Ibrahim, Ziad; Panne, Daniel; Rousseaux, Sophie; Zhao, Yingming; Khochbin, Saadi Nut Directs p300-Dependent, Genome-Wide Ħ4 Hyperacetylation in Male Germ Cells Article de journal Cell Reports, 24 (13), p. 3477–3487.e6, 2018, ISSN: 2211-1247. @article{shiota_nut_2018, title = {Nut Directs p300-Dependent, Genome-Wide Ħ4 Hyperacetylation in Male Germ Cells}, author = {Hitoshi Shiota and Sophie Barral and Thierry Buchou and Minjia Tan and Yohann Couté and Guillaume Charbonnier and Nicolas Reynoird and Fayçal Boussouar and Matthieu Gérard and Mingrui Zhu and Lisa Bargier and Denis Puthier and Florent Chuffart and Ekaterina Bourova-Flin and Sarah Picaud and Panagis Filippakopoulos and Afsaneh Goudarzi and Ziad Ibrahim and Daniel Panne and Sophie Rousseaux and Yingming Zhao and Saadi Khochbin}, doi = {10.1016/j.celrep.2018.08.069}, issn = {2211-1247}, year = {2018}, date = {2018-09-01}, journal = {Cell Reports}, volume = {24}, number = {13}, pages = {3477--3487.e6}, abstract = {Nuclear protein in testis (Nut) is a universal oncogenic driver in the highly aggressive NUT midline carcinoma, whose physiological function in male germ cells has been unclear. Here we show that expression of Nut is normally restricted to post-meiotic spermatogenic cells, where its presence triggers p300-dependent genome-wide histone H4 hyperacetylation, which is essential for the completion of histone-to-protamine exchange. Accordingly, the inactivation of Nut induces male sterility with spermatogenesis arrest at the histone-removal stage. Nut uses p300 and/or CBP to enhance acetylation of H4 at both K5 and K8, providing binding sites for the first bromodomain of Brdt, the testis-specific member of the BET family, which subsequently mediates genome-wide histone removal. Altogether, our data reveal the detailed molecular basis of the global histone hyperacetylation wave, which occurs before the final compaction of the male genome.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Nuclear protein in testis (Nut) is a universal oncogenic driver in the highly aggressive NUT midline carcinoma, whose physiological function in male germ cells has been unclear. Here we show that expression of Nut is normally restricted to post-meiotic spermatogenic cells, where its presence triggers p300-dependent genome-wide histone H4 hyperacetylation, which is essential for the completion of histone-to-protamine exchange. Accordingly, the inactivation of Nut induces male sterility with spermatogenesis arrest at the histone-removal stage. Nut uses p300 and/or CBP to enhance acetylation of H4 at both K5 and K8, providing binding sites for the first bromodomain of Brdt, the testis-specific member of the BET family, which subsequently mediates genome-wide histone removal. Altogether, our data reveal the detailed molecular basis of the global histone hyperacetylation wave, which occurs before the final compaction of the male genome. |
Nguyen, Minh Vu Chuong; Adrait, Annie; Baillet, Athan; Trocmé, Candice; Gottenberg, Jacques-Eric; Gaudin, Philippe Joint, Bone, Spine: Revue Du Rhumatisme, 2018, ISSN: 1778-7254. @article{nguyen_identification_2018, title = {Identification of cartilage oligomeric matrix protein as biomarker predicting abatacept response in rheumatoid arthritis patients with insufficient response to a first anti-TNFα treatment}, author = {Minh Vu Chuong Nguyen and Annie Adrait and Athan Baillet and Candice Trocmé and Jacques-Eric Gottenberg and Philippe Gaudin}, doi = {10.1016/j.jbspin.2018.09.005}, issn = {1778-7254}, year = {2018}, date = {2018-09-01}, journal = {Joint, Bone, Spine: Revue Du Rhumatisme}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mato, José M; Elortza, Félix; Lu, Shelly C; Brun, Virginie; Paradela, Alberto; Corrales, Fernando J Liver cancer-associated changes to the proteome: what deserves clinical focus? Article de journal Expert Review of Proteomics, 15 (9), p. 749–756, 2018, ISSN: 1744-8387. @article{mato_liver_2018, title = {Liver cancer-associated changes to the proteome: what deserves clinical focus?}, author = {José M Mato and Félix Elortza and Shelly C Lu and Virginie Brun and Alberto Paradela and Fernando J Corrales}, doi = {10.1080/14789450.2018.1521277}, issn = {1744-8387}, year = {2018}, date = {2018-09-01}, journal = {Expert Review of Proteomics}, volume = {15}, number = {9}, pages = {749--756}, abstract = {INTRODUCTION: Hepatocellular carcinoma (HCC) is recognized as the fifth most common neoplasm and currently represents the second leading form of cancer-related death worldwide. Despite great progress has been done in the understanding of its pathogenesis, HCC represents a heavy societal and economic burden as most patients are still diagnosed at advanced stages and the 5-year survival rate remain below 20%. Early detection and revolutionary therapies that rely on the discovery of new molecular biomarkers and therapeutic targets are therefore urgently needed to develop precision medicine strategies for a more efficient management of patients. Areas covered: This review intends to comprehensively analyse the proteomics-based research conducted in the last few years to address some of the principal still open riddles in HCC biology, based on the identification of molecular drivers of tumor progression and metastasis. Expert commentary: The technical advances in mass spectrometry experienced in the last decade have significantly improved the analytical capacity of proteome wide studies. Large-scale protein and protein variant (post-translational modifications) identification and quantification have allowed detailed dissections of molecular mechanisms underlying HCC progression and are already paving the way for the identification of clinically relevant proteins and the development of their use on patient care.}, keywords = {}, pubstate = {published}, tppubtype = {article} } INTRODUCTION: Hepatocellular carcinoma (HCC) is recognized as the fifth most common neoplasm and currently represents the second leading form of cancer-related death worldwide. Despite great progress has been done in the understanding of its pathogenesis, HCC represents a heavy societal and economic burden as most patients are still diagnosed at advanced stages and the 5-year survival rate remain below 20%. Early detection and revolutionary therapies that rely on the discovery of new molecular biomarkers and therapeutic targets are therefore urgently needed to develop precision medicine strategies for a more efficient management of patients. Areas covered: This review intends to comprehensively analyse the proteomics-based research conducted in the last few years to address some of the principal still open riddles in HCC biology, based on the identification of molecular drivers of tumor progression and metastasis. Expert commentary: The technical advances in mass spectrometry experienced in the last decade have significantly improved the analytical capacity of proteome wide studies. Large-scale protein and protein variant (post-translational modifications) identification and quantification have allowed detailed dissections of molecular mechanisms underlying HCC progression and are already paving the way for the identification of clinically relevant proteins and the development of their use on patient care. |
Locard-Paulet, Marie; Parra, Julien; Albigot, Renaud; Mouton-Barbosa, Emmanuelle; Bardi, Laurent; Burlet-Schiltz, Odile; Marcoux, Julien VisioProt-MS: interactive 2D maps from intact protein mass spectrometry Article de journal Bioinformatics, 2018. @article{Locard-Paulet2018, title = {VisioProt-MS: interactive 2D maps from intact protein mass spectrometry}, author = {Marie Locard-Paulet and Julien Parra and Renaud Albigot and Emmanuelle Mouton-Barbosa and Laurent Bardi and Odile Burlet-Schiltz and Julien Marcoux}, doi = {10.1093/bioinformatics/bty680}, year = {2018}, date = {2018-08-06}, journal = {Bioinformatics}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Vinel, Claire; Lukjanenko, Laura; Batut, Aurélie; Deleruyelle, Simon; Pradère, Jean-Philippe; Gonidec, Sophie Le; Dortignac, Alizée; Geoffre, Nancy; Pereira, Ophélie; Karaz, Sonia; Lee, Umji; Camus, Mylène; Chaoui, Karima; Mouisel, Etienne; Bigot, Anne; Mouly, Vincent; Vigneau, Mathieu; Pagano, Allan F; Chopard, Angèle; Pillard, Fabien; Guyonnet, Sophie; Cesari, Matteo; Burlet-Schiltz, Odile; Pahor, Marco; Feige, Jérôme N; Vellas, Bruno; Valet, Philippe; Dray, Cédric The exerkine apelin reverses age-associated sarcopenia Article de journal Nat Med., 24 (9), p. 1360-71, 2018. @article{Vinel2018, title = {The exerkine apelin reverses age-associated sarcopenia}, author = {Claire Vinel and Laura Lukjanenko and Aurélie Batut and Simon Deleruyelle and Jean-Philippe Pradère and Sophie Le Gonidec and Alizée Dortignac and Nancy Geoffre and Ophélie Pereira and Sonia Karaz and Umji Lee and Mylène Camus and Karima Chaoui and Etienne Mouisel and Anne Bigot and Vincent Mouly and Mathieu Vigneau and Allan F. Pagano and Angèle Chopard and Fabien Pillard and Sophie Guyonnet and Matteo Cesari and Odile Burlet-Schiltz and Marco Pahor and Jérôme N. Feige and Bruno Vellas and Philippe Valet and Cédric Dray}, doi = {10.1038/s41591-018-0131-6}, year = {2018}, date = {2018-07-30}, journal = {Nat Med.}, volume = {24}, number = {9}, pages = {1360-71}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Jonik-Nowak, Beata; Menneteau, Thoma; Fesquet, Didier; Baldin, Véronique; Bonne-Andrea, Catherine; Méchali, Francisca; Fabre, Bertrand; Boisguerin, Prisca; de Rossi, Sylvain; Henriquet, Corinne; Pugnière, Martine; Ducoux-Petit, Manuelle; Burlet-Schiltz, Odile; Lamond, Angus I; Fort, Philippe; Boulon, Séverine; Bousquet, Mari-Pierre; Coux, Olivier PIP30/FAM192A is a novel regulator of the nuclear proteasome activator PA28γ Article de journal Proc Natl Acad Sci U S A., 115 (28), p. E6477-E6486, 2018. @article{Jonik-Nowak2018, title = {PIP30/FAM192A is a novel regulator of the nuclear proteasome activator PA28γ}, author = {Beata Jonik-Nowak and Thoma Menneteau and Didier Fesquet and Véronique Baldin and Catherine Bonne-Andrea and Francisca Méchali and Bertrand Fabre and Prisca Boisguerin and Sylvain de Rossi and Corinne Henriquet and Martine Pugnière and Manuelle Ducoux-Petit and Odile Burlet-Schiltz and Angus I. Lamond and Philippe Fort and Séverine Boulon and Mari-Pierre Bousquet and Olivier Coux}, doi = {10.1073/pnas.1722299115}, year = {2018}, date = {2018-07-10}, journal = {Proc Natl Acad Sci U S A.}, volume = {115}, number = {28}, pages = {E6477-E6486}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Gaud, Guillaume; Roncagalli, Romain; Chaoui, Karima; Bernard, Isabelle; andCéline Colacios, Julien Familiades; Kassem, Sahar; Monsarrat, Bernard; Burlet-Schiltz, Odile; de Peredo, Anne Gonzalez; Malissen, Bernard; Saoudi, Abdelhadi The costimulatory molecule CD226 signals through VAV1 to amplify TCR signals and promote IL-17 production by CD4+ T cells Article de journal 11 (538), p. eaar3083, 2018. @article{Gaud2018, title = {The costimulatory molecule CD226 signals through VAV1 to amplify TCR signals and promote IL-17 production by CD4+ T cells}, author = {Guillaume Gaud and Romain Roncagalli and Karima Chaoui and Isabelle Bernard and Julien Familiades andCéline Colacios and Sahar Kassem and Bernard Monsarrat and Odile Burlet-Schiltz and Anne Gonzalez de Peredo and Bernard Malissen and Abdelhadi Saoudi}, doi = {10.1126/scisignal.aar3083}, year = {2018}, date = {2018-07-10}, volume = {11}, number = {538}, pages = {eaar3083}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Behrens, Christina; Biela, Inna; andThomas Botzanowski, Stéphanie Petiot-Bécard; Cianférani, Sarah; Sager, Christoph P; Klebe, Gerhard; Heine, Andreas; Reuter, Klaus Homodimer Architecture of QTRT2, the Noncatalytic Subunit of the Eukaryotic tRNA-Guanine Transglycosylase Article de journal Biochemistry, 57 (26), p. 3953–3965, 2018. @article{Behrens2018, title = {Homodimer Architecture of QTRT2, the Noncatalytic Subunit of the Eukaryotic tRNA-Guanine Transglycosylase}, author = {Christina Behrens and Inna Biela and Stéphanie Petiot-Bécard andThomas Botzanowski and Sarah Cianférani and Christoph P. Sager and Gerhard Klebe and Andreas Heine and Klaus Reuter}, doi = {10.1021/acs.biochem.8b00294}, year = {2018}, date = {2018-06-04}, journal = {Biochemistry}, volume = {57}, number = {26}, pages = {3953–3965}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fröhlich, Tony; Hahn, Friedrich; Belmudes, Lucid; Leidenberger, Maria; Friedrich, Oliver; Kappes, Barbara; Couté, Yohann; Marschall, Manfred; Tsogoeva, Svetlana B Synthesis of Artemisinin-Derived Dimers, Trimers and Dendrimers: Investigation of Their Antimalarial and Antiviral Activities Including Putative Mechanisms of Action Article de journal Chemistry (Weinheim an Der Bergstrasse, Germany), 24 (32), p. 8103–8113, 2018, ISSN: 1521-3765. @article{frohlich_synthesis_2018, title = {Synthesis of Artemisinin-Derived Dimers, Trimers and Dendrimers: Investigation of Their Antimalarial and Antiviral Activities Including Putative Mechanisms of Action}, author = {Tony Fröhlich and Friedrich Hahn and Lucid Belmudes and Maria Leidenberger and Oliver Friedrich and Barbara Kappes and Yohann Couté and Manfred Marschall and Svetlana B Tsogoeva}, doi = {10.1002/chem.201800729}, issn = {1521-3765}, year = {2018}, date = {2018-06-01}, journal = {Chemistry (Weinheim an Der Bergstrasse, Germany)}, volume = {24}, number = {32}, pages = {8103--8113}, abstract = {Generation of dimers, trimers and dendrimers of bioactive compounds is an approach that has recently been developed for the discovery of new potent drug candidates. Herein, we present the synthesis of new artemisinin-derived dimers and dendrimers and investigate their action against malaria parasite Plasmodium falciparum 3D7 strain and human cytomegalovirus (HCMV). Dimer 7 was the most active compound (EC50 1.4 nm) in terms of antimalarial efficacy and was even more effective than the standard drugs dihydroartemisinin (EC50 2.4 nm), artesunic acid (EC50 8.9 nm) and chloroquine (EC50 9.8 nm). Trimer 4 stood out as the most active agent against HCMV in vitro replication and exerted an EC50 value of 0.026 μm, representing an even higher activity than the two reference drugs ganciclovir (EC50 2.60 μm) and artesunic acid (EC50 5.41 μm). In addition, artemisinin-derived dimer 13 and trimer 15 were for the first time both immobilized on TOYOPEARL AF-Amino-650M beads and used for mass spectrometry-based target identification experiments using total lysates of HCMV-infected primary human fibroblasts. Two major groups of novel target candidates, namely cytoskeletal and mitochondrial proteins were obtained. Two putatively compound-binding viral proteins, namely major capsid protein (MCP) and envelope glycoprotein pUL132, which are both essential for HCMV replication, were identified.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Generation of dimers, trimers and dendrimers of bioactive compounds is an approach that has recently been developed for the discovery of new potent drug candidates. Herein, we present the synthesis of new artemisinin-derived dimers and dendrimers and investigate their action against malaria parasite Plasmodium falciparum 3D7 strain and human cytomegalovirus (HCMV). Dimer 7 was the most active compound (EC50 1.4 nm) in terms of antimalarial efficacy and was even more effective than the standard drugs dihydroartemisinin (EC50 2.4 nm), artesunic acid (EC50 8.9 nm) and chloroquine (EC50 9.8 nm). Trimer 4 stood out as the most active agent against HCMV in vitro replication and exerted an EC50 value of 0.026 μm, representing an even higher activity than the two reference drugs ganciclovir (EC50 2.60 μm) and artesunic acid (EC50 5.41 μm). In addition, artemisinin-derived dimer 13 and trimer 15 were for the first time both immobilized on TOYOPEARL AF-Amino-650M beads and used for mass spectrometry-based target identification experiments using total lysates of HCMV-infected primary human fibroblasts. Two major groups of novel target candidates, namely cytoskeletal and mitochondrial proteins were obtained. Two putatively compound-binding viral proteins, namely major capsid protein (MCP) and envelope glycoprotein pUL132, which are both essential for HCMV replication, were identified. |
Jacob, Laurent; Combes, Florence; Burger, Thomas PEPA test: fast and powerful differential analysis from relative quantitative proteomics data using shared peptides Article de journal Biostatistics (Oxford, England), 2018, ISSN: 1468-4357. @article{jacob_pepa_2018, title = {PEPA test: fast and powerful differential analysis from relative quantitative proteomics data using shared peptides}, author = {Laurent Jacob and Florence Combes and Thomas Burger}, doi = {10.1093/biostatistics/kxy021}, issn = {1468-4357}, year = {2018}, date = {2018-06-01}, journal = {Biostatistics (Oxford, England)}, abstract = {We propose a new hypothesis test for the differential abundance of proteins in mass-spectrometry based relative quantification. An important feature of this type of high-throughput analyses is that it involves an enzymatic digestion of the sample proteins into peptides prior to identification and quantification. Due to numerous homology sequences, different proteins can lead to peptides with identical amino acid chains, so that their parent protein is ambiguous. These so-called shared peptides make the protein-level statistical analysis a challenge and are often not accounted for. In this article, we use a linear model describing peptide-protein relationships to build a likelihood ratio test of differential abundance for proteins. We show that the likelihood ratio statistic can be computed in linear time with the number of peptides. We also provide the asymptotic null distribution of a regularized version of our statistic. Experiments on both real and simulated datasets show that our procedures outperforms state-of-the-art methods. The procedures are available via the pepa.test function of the DAPAR Bioconductor R package.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We propose a new hypothesis test for the differential abundance of proteins in mass-spectrometry based relative quantification. An important feature of this type of high-throughput analyses is that it involves an enzymatic digestion of the sample proteins into peptides prior to identification and quantification. Due to numerous homology sequences, different proteins can lead to peptides with identical amino acid chains, so that their parent protein is ambiguous. These so-called shared peptides make the protein-level statistical analysis a challenge and are often not accounted for. In this article, we use a linear model describing peptide-protein relationships to build a likelihood ratio test of differential abundance for proteins. We show that the likelihood ratio statistic can be computed in linear time with the number of peptides. We also provide the asymptotic null distribution of a regularized version of our statistic. Experiments on both real and simulated datasets show that our procedures outperforms state-of-the-art methods. The procedures are available via the pepa.test function of the DAPAR Bioconductor R package. |
Denadai-Souza, Alexandre; Bonnart, Chrystelle; Tapias, Núria Solà; Marcellin, Marlène; andLaurent Alric, Brendan Gilmore; Bonnet, Delphine; Burlet-Schiltz, Odile; Hollenberg, Morley D; Vergnolle, Nathalie; Deraison, Céline Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease Article de journal Sci Rep, 8 (1), p. 7834, 2018. @article{Denadai-Souza2018, title = {Functional Proteomic Profiling of Secreted Serine Proteases in Health and Inflammatory Bowel Disease}, author = {Alexandre Denadai-Souza and Chrystelle Bonnart and Núria Solà Tapias and Marlène Marcellin and Brendan Gilmore andLaurent Alric and Delphine Bonnet and Odile Burlet-Schiltz and Morley D. Hollenberg and Nathalie Vergnolle and Céline Deraison}, doi = {10.1038/s41598-018-26282-y}, year = {2018}, date = {2018-05-18}, journal = {Sci Rep}, volume = {8}, number = {1}, pages = {7834}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Lefebvre, Cyril; Boulon, Richard; Ducoux, Manuelle; Gavalda, Sabine; Laval, Françoise; Jamet, Stevie; Eynard, Nathalie; Lemassu, Anne; Cam, Kaymeuang; Bousquet, Marie-Pierre; Bardou, Fabienne; Burlet-Schiltz, Odile; Daffé, Mamadou; Quémard, Annaïck HadD, a novel fatty acid synthase type II protein, is essential for alpha- and epoxy-mycolic acid biosynthesis and mycobacterial fitness Article de journal Sci Rep, 8 (1), p. 6034, 2018. @article{Lefebvre2018, title = {HadD, a novel fatty acid synthase type II protein, is essential for alpha- and epoxy-mycolic acid biosynthesis and mycobacterial fitness}, author = {Cyril Lefebvre and Richard Boulon and Manuelle Ducoux and Sabine Gavalda and Françoise Laval and Stevie Jamet and Nathalie Eynard and Anne Lemassu and Kaymeuang Cam and Marie-Pierre Bousquet and Fabienne Bardou and Odile Burlet-Schiltz and Mamadou Daffé and Annaïck Quémard}, doi = {10.1038/s41598-018-24380-5}, year = {2018}, date = {2018-04-16}, journal = {Sci Rep}, volume = {8}, number = {1}, pages = {6034}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cayrol, Corinne; Duval, Anaïs; Schmitt, Pauline; Roga, Stéphane; Stella, Mylène Camusand Alexandre; Burlet-Schiltz, Odile; Gonzalez de Peredo, Anne ; Girard, Jean-Philippe Environmental allergens induce allergic inflammation through proteolytic maturation of IL-33 Article de journal Nat Immunol, 19 (4), p. 375-385, 2018. @article{Cayrol2018, title = {Environmental allergens induce allergic inflammation through proteolytic maturation of IL-33}, author = {Corinne Cayrol and Anaïs Duval and Pauline Schmitt and Stéphane Roga and Mylène Camusand Alexandre Stella and Odile Burlet-Schiltz and Anne {Gonzalez de Peredo} and Jean-Philippe Girard}, doi = {10.1038/s41590-018-0067-5 }, year = {2018}, date = {2018-03-21}, journal = {Nat Immunol}, volume = {19}, number = {4}, pages = {375-385}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Garcia, Lény; Girod, Marion; Rompais, Magali; Dugourd, Philippe; Carapito, Christine; Lemoine, Jérôme Data-Independent Acquisition Coupled to Visible Laser-Induced Dissociation at 473 nm (DIA-LID) for Peptide-Centric Specific Analysis of Cysteine-Containing Peptide Subset Article de journal Anal Chem., 90 (6), p. 3928-3935, 2018. @article{Garcia2018, title = {Data-Independent Acquisition Coupled to Visible Laser-Induced Dissociation at 473 nm (DIA-LID) for Peptide-Centric Specific Analysis of Cysteine-Containing Peptide Subset}, author = {Lény Garcia and Marion Girod and Magali Rompais and Philippe Dugourd and Christine Carapito and Jérôme Lemoine}, doi = {10.1021/acs.analchem.7b04821}, year = {2018}, date = {2018-03-20}, journal = {Anal Chem.}, volume = {90}, number = {6}, pages = {3928-3935}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dovgan, I; Erb, S; Hessmann, S; Ursuegui, S; Michel, C; Muller, C; Chaubet, G; Cianférani, S; Wagner, A Arginine-selective bioconjugation with 4-azidophenyl glyoxal: application to the single and dual functionalisation of native antibodies Article de journal Org Biomol Chem., 16 (8), p. 1305-1311, 2018. @article{RN1311, title = {Arginine-selective bioconjugation with 4-azidophenyl glyoxal: application to the single and dual functionalisation of native antibodies}, author = {I Dovgan and S Erb and S Hessmann and S Ursuegui and C Michel and C Muller and G Chaubet and S Cianférani and A Wagner}, doi = {10.1039/c7ob02844j}, year = {2018}, date = {2018-02-21}, journal = {Org Biomol Chem.}, volume = {16}, number = {8}, pages = {1305-1311}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Issa, N; Guillaumot, N; Lauret, E; Matt, N; Schaeffer-Reiss, C; Van Dorsselaer, A; Reichhart, J M; Veillard, F The Circulating Protease Persephone Is an Immune Sensor for Microbial Proteolytic Activities Upstream of the Drosophila Toll Pathway Article de journal Mol Cell, 69 (4), p. 539-550 e6, 2018. @article{RN1312, title = {The Circulating Protease Persephone Is an Immune Sensor for Microbial Proteolytic Activities Upstream of the Drosophila Toll Pathway}, author = {N Issa and N Guillaumot and E Lauret and N Matt and C Schaeffer-Reiss and A {Van Dorsselaer} and J M Reichhart and F Veillard}, doi = {10.1016/j.molcel.2018.01.029}, year = {2018}, date = {2018-02-15}, journal = {Mol Cell}, volume = {69}, number = {4}, pages = {539-550 e6}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ehkirch, A; D'Atri, V; Rouvière, F; Hernandez-Alba, O; Goyon, A; Colas, O; Sarrut, M; Beck, A; Guillarme, D; Heinisch, S; Cianférani, S An online four-dimensional HICxSEC-IMxMS methodology for proof-of-concept characterization of antibody drug conjugates Article de journal Anal Chem., 90 (3), p. 1578-1586, 2018. @article{RN1307, title = {An online four-dimensional HICxSEC-IMxMS methodology for proof-of-concept characterization of antibody drug conjugates}, author = {A Ehkirch and V D'Atri and F Rouvière and O Hernandez-Alba and A Goyon and O Colas and M Sarrut and A Beck and D Guillarme and S Heinisch and S Cianférani}, doi = {10.1021/acs.analchem.7b02110}, year = {2018}, date = {2018-02-06}, journal = {Anal Chem.}, volume = {90}, number = {3}, pages = {1578-1586}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Lacombe, Maud; Marie-Desvergne, Caroline; Combes, Florence; Kraut, Alexandra; Bruley, Christophe; Vandenbrouck, Yves; Mossuz, Véronique Chamel; Couté, Yohann; Brun, Virginie Proteomic characterization of human exhaled breath condensate Article de journal Journal of Breath Research, 12 (2), p. 021001, 2018, ISSN: 1752-7163. @article{lacombe_proteomic_2018, title = {Proteomic characterization of human exhaled breath condensate}, author = {Maud Lacombe and Caroline Marie-Desvergne and Florence Combes and Alexandra Kraut and Christophe Bruley and Yves Vandenbrouck and Véronique Chamel Mossuz and Yohann Couté and Virginie Brun}, doi = {10.1088/1752-7163/aa9e71}, issn = {1752-7163}, year = {2018}, date = {2018-02-01}, journal = {Journal of Breath Research}, volume = {12}, number = {2}, pages = {021001}, abstract = {To improve biomedical knowledge and to support biomarker discovery studies, it is essential to establish comprehensive proteome maps for human tissues and biofluids, and to make them publicly accessible. In this study, we performed an in-depth proteomics characterization of exhaled breath condensate (EBC), a sample obtained non-invasively by condensation of exhaled air that contains submicron droplets of airway lining fluid. Two pooled samples of EBC, each obtained from 10 healthy donors, were processed using a straightforward protocol based on sample lyophilization, in-gel digestion and liquid chromatography tandem-mass spectrometry analysis. Two 'technical' control samples were processed in parallel to the pooled samples to correct for exogenous protein contamination. A total of 229 unique proteins were identified in EBC among which 153 proteins were detected in both EBC pooled samples. A detailed bioinformatics analysis of these 153 proteins showed that most of the proteins identified corresponded to proteins secreted in the respiratory tract (lung, bronchi). Eight proteins were salivary proteins. Our dataset is described and has been made accessible through the ProteomeXchange database (dataset identifier: PXD007591) and is expected to be useful for future MS-based biomarker studies using EBC as the diagnostic specimen.}, keywords = {}, pubstate = {published}, tppubtype = {article} } To improve biomedical knowledge and to support biomarker discovery studies, it is essential to establish comprehensive proteome maps for human tissues and biofluids, and to make them publicly accessible. In this study, we performed an in-depth proteomics characterization of exhaled breath condensate (EBC), a sample obtained non-invasively by condensation of exhaled air that contains submicron droplets of airway lining fluid. Two pooled samples of EBC, each obtained from 10 healthy donors, were processed using a straightforward protocol based on sample lyophilization, in-gel digestion and liquid chromatography tandem-mass spectrometry analysis. Two 'technical' control samples were processed in parallel to the pooled samples to correct for exogenous protein contamination. A total of 229 unique proteins were identified in EBC among which 153 proteins were detected in both EBC pooled samples. A detailed bioinformatics analysis of these 153 proteins showed that most of the proteins identified corresponded to proteins secreted in the respiratory tract (lung, bronchi). Eight proteins were salivary proteins. Our dataset is described and has been made accessible through the ProteomeXchange database (dataset identifier: PXD007591) and is expected to be useful for future MS-based biomarker studies using EBC as the diagnostic specimen. |
Grzela, R; Nusbaum, J; Fieulaine, S; Lavecchia, F; Desmadril, M; Nhiri, N; Van Dorsselaer, A; Cianférani, S; Jacquet, E; Meinnel, T; Giglione, C Peptide deformylases from Vibrio parahaemolyticus phage and bacteria display similar deformylase activity and inhibitor binding clefts Article de journal Biochim Biophys Acta., 1866 (2), p. 348-355, 2018. @article{RN1310, title = {Peptide deformylases from Vibrio parahaemolyticus phage and bacteria display similar deformylase activity and inhibitor binding clefts}, author = {R Grzela and J Nusbaum and S Fieulaine and F Lavecchia and M Desmadril and N Nhiri and A {Van Dorsselaer} and S Cianférani and E Jacquet and T Meinnel and C Giglione}, doi = {10.1016/j.bbapap.2017.10.007}, year = {2018}, date = {2018-02-01}, journal = {Biochim Biophys Acta.}, volume = {1866}, number = {2}, pages = {348-355}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
A, Métais; I, Lamsoul; A, Melet; S, Uttenweiler-Joseph; R, Poincloux; S, Stefanovic; A, Valière; de A, Gonzalez Peredo; A, Stella; O, Burlet-Schiltz; S, Zaffran; PG, Lutz; C, Moog-Lutz Asb2α-Filamin A Axis Is Essential for Actin Cytoskeleton Remodeling During Heart Development Article de journal Circ Res., 122 (6), p. 34-48, 2018. @article{A2018, title = {Asb2α-Filamin A Axis Is Essential for Actin Cytoskeleton Remodeling During Heart Development}, author = {Métais A and Lamsoul I and Melet A and Uttenweiler-Joseph S and Poincloux R and Stefanovic S and Valière A and Gonzalez de Peredo A and Stella A and Burlet-Schiltz O and Zaffran S and Lutz PG and Moog-Lutz C}, doi = {10.1161/CIRCRESAHA.117.312015}, year = {2018}, date = {2018-01-26}, journal = {Circ Res.}, volume = {122}, number = {6}, pages = {34-48}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mori, M; Kovalenko, L; Malancona, S; Saladini, F; Forni, De D; Pires, M; Humbert, N; Real, E; Botzanowski, T; Cianférani, S; Giannini, A; Lang, MC. Dasso; Cugia, G; Poddesu, B; Lori, F; Zazzi, M; Harper, S; Summa, V; Mely, Y; Botta, M Structure-Based Identification of HIV-1 Nucleocapsid Protein Inhibitors Active against Wild-Type and Drug-Resistant HIV-1 Strains Article de journal ACS Chem Biol., 13 (1), p. 253-266, 2018. @article{RN1308, title = {Structure-Based Identification of HIV-1 Nucleocapsid Protein Inhibitors Active against Wild-Type and Drug-Resistant HIV-1 Strains}, author = {M Mori and L Kovalenko and S Malancona and F Saladini and D De Forni and M Pires and N Humbert and E Real and T Botzanowski and S Cianférani and A Giannini and MC. Dasso Lang and G Cugia and B Poddesu and F Lori and M Zazzi and S Harper and V Summa and Y Mely and M Botta}, doi = {10.1021/acschembio.7b00907}, year = {2018}, date = {2018-01-19}, journal = {ACS Chem Biol.}, volume = {13}, number = {1}, pages = {253-266}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Husson, G; Delangle, A; O'Hara, J; Cianférani, S; Gervais, A; Van Dorsselaer, A; Bracewell, D; Carapito, C Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development Article de journal Anal Chem., 90 (2), p. 1241-1247, 2018. @article{RN1306, title = {Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development}, author = {G Husson and A Delangle and J O'Hara and S Cianférani and A Gervais and A {Van Dorsselaer} and D Bracewell and C Carapito}, doi = {10.1021/acs.analchem.7b03965 }, year = {2018}, date = {2018-01-16}, journal = {Anal Chem.}, volume = {90}, number = {2}, pages = {1241-1247}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
L, Favre; A, Ortalo-Magné; C, Pichereaux; A, Gargaros; O, Burlet-Schiltz; V, Cotelle; G., Culioli Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8 Article de journal Biofouling, 34 (2), p. 132-148, 2018. @article{L2018, title = {Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8}, author = {Favre L and Ortalo-Magné A and Pichereaux C and Gargaros A and Burlet-Schiltz O and Cotelle V and Culioli G.}, doi = {10.1080/08927014.2017.1413551}, year = {2018}, date = {2018-01-10}, journal = {Biofouling}, volume = {34}, number = {2}, pages = {132-148}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rayapuram, Naganand; Bigeard, Jean; Alhoraibi, Hanna; Bonhomme, Ludovic; Hesse, Anne-Marie; Vinh, Joëlle; Hirt, Heribert; Pflieger, Delphine Quantitative Phosphoproteomic Analysis Reveals Shared and Specific Targets ofArabidopsisMitogen-Activated Protein Kinases (MAPKs) MPK3, MPK4, and MPK6 Article de journal Molecular & cellular proteomics: MCP, 17 (1), p. 61–80, 2018, ISSN: 1535-9484. @article{rayapuram_quantitative_2018, title = {Quantitative Phosphoproteomic Analysis Reveals Shared and Specific Targets ofArabidopsisMitogen-Activated Protein Kinases (MAPKs) MPK3, MPK4, and MPK6}, author = {Naganand Rayapuram and Jean Bigeard and Hanna Alhoraibi and Ludovic Bonhomme and Anne-Marie Hesse and Joëlle Vinh and Heribert Hirt and Delphine Pflieger}, doi = {10.1074/mcp.RA117.000135}, issn = {1535-9484}, year = {2018}, date = {2018-01-01}, journal = {Molecular & cellular proteomics: MCP}, volume = {17}, number = {1}, pages = {61--80}, abstract = {In Arabidopsis, mitogen-activated protein kinases MPK3, MPK4, and MPK6 constitute essential relays for a variety of functions including cell division, development and innate immunity. Although some substrates of MPK3, MPK4 and MPK6 have been identified, the picture is still far from complete. To identify substrates of these MAPKs likely involved in cell division, growth and development we compared the phosphoproteomes of wild-type andmpk3,mpk4, andmpk6.To study the function of these MAPKs in innate immunity, we analyzed their phosphoproteomes following microbe-associated molecular pattern (MAMP) treatment. Partially overlapping substrates were retrieved for all three MAPKs, showing target specificity to one, two or all three MAPKs in different biological processes. More precisely, our results illustrate the fact that the entity to be defined as a specific or a shared substrate for MAPKs is not a phosphoprotein but a particular (S/T)P phosphorylation site in a given protein. One hundred fifty-two peptides were identified to be differentially phosphorylated in response to MAMP treatment and/or when compared between genotypes and 70 of them could be classified as putative MAPK targets. Biochemical analysis of a number of putative MAPK substrates by phosphorylation and interaction assays confirmed the global phosphoproteome approach. Our study also expands the set of MAPK substrates to involve other protein kinases, including calcium-dependent (CDPK) and sugar nonfermenting (SnRK) protein kinases.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In Arabidopsis, mitogen-activated protein kinases MPK3, MPK4, and MPK6 constitute essential relays for a variety of functions including cell division, development and innate immunity. Although some substrates of MPK3, MPK4 and MPK6 have been identified, the picture is still far from complete. To identify substrates of these MAPKs likely involved in cell division, growth and development we compared the phosphoproteomes of wild-type andmpk3,mpk4, andmpk6.To study the function of these MAPKs in innate immunity, we analyzed their phosphoproteomes following microbe-associated molecular pattern (MAMP) treatment. Partially overlapping substrates were retrieved for all three MAPKs, showing target specificity to one, two or all three MAPKs in different biological processes. More precisely, our results illustrate the fact that the entity to be defined as a specific or a shared substrate for MAPKs is not a phosphoprotein but a particular (S/T)P phosphorylation site in a given protein. One hundred fifty-two peptides were identified to be differentially phosphorylated in response to MAMP treatment and/or when compared between genotypes and 70 of them could be classified as putative MAPK targets. Biochemical analysis of a number of putative MAPK substrates by phosphorylation and interaction assays confirmed the global phosphoproteome approach. Our study also expands the set of MAPK substrates to involve other protein kinases, including calcium-dependent (CDPK) and sugar nonfermenting (SnRK) protein kinases. |
Burger, Thomas Gentle Introduction to the Statistical Foundations of False Discovery Rate in Quantitative Proteomics Article de journal Journal of Proteome Research, 17 (1), p. 12–22, 2018, ISSN: 1535-3907. @article{burger_gentle_2018, title = {Gentle Introduction to the Statistical Foundations of False Discovery Rate in Quantitative Proteomics}, author = {Thomas Burger}, doi = {10.1021/acs.jproteome.7b00170}, issn = {1535-3907}, year = {2018}, date = {2018-01-01}, journal = {Journal of Proteome Research}, volume = {17}, number = {1}, pages = {12--22}, abstract = {The vocabulary of theoretical statistics can be difficult to embrace from the viewpoint of computational proteomics research, even though the notions it conveys are essential to publication guidelines. For example, "adjusted p-values", "q-values", and "false discovery rates" are essentially similar concepts, whereas "false discovery rate" and "false discovery proportion" must not be confused, even though "rate" and "proportion" are related in everyday language. In the interdisciplinary context of proteomics, such subtleties may cause misunderstandings. This article aims to provide an easy-to-understand explanation of these four notions (and a few other related ones). Their statistical foundations are dealt with from a perspective that largely relies on intuition, addressing mainly protein quantification but also, to some extent, peptide identification. In addition, a clear distinction is made between concepts that define an individual property (i.e., related to a peptide or a protein) and those that define a set property (i.e., related to a list of peptides or proteins).}, keywords = {}, pubstate = {published}, tppubtype = {article} } The vocabulary of theoretical statistics can be difficult to embrace from the viewpoint of computational proteomics research, even though the notions it conveys are essential to publication guidelines. For example, "adjusted p-values", "q-values", and "false discovery rates" are essentially similar concepts, whereas "false discovery rate" and "false discovery proportion" must not be confused, even though "rate" and "proportion" are related in everyday language. In the interdisciplinary context of proteomics, such subtleties may cause misunderstandings. This article aims to provide an easy-to-understand explanation of these four notions (and a few other related ones). Their statistical foundations are dealt with from a perspective that largely relies on intuition, addressing mainly protein quantification but also, to some extent, peptide identification. In addition, a clear distinction is made between concepts that define an individual property (i.e., related to a peptide or a protein) and those that define a set property (i.e., related to a list of peptides or proteins). |
Kennani, Sara El; Crespo, Marion; Govin, Jérôme; Pflieger, Delphine Proteomic Analysis of Histone Variants and Their PTMs: Strategies and Pitfalls Article de journal Proteomes, 6 (3), 2018, ISSN: 2227-7382. @article{el_kennani_proteomic_2018, title = {Proteomic Analysis of Histone Variants and Their PTMs: Strategies and Pitfalls}, author = {Sara El Kennani and Marion Crespo and Jérôme Govin and Delphine Pflieger}, doi = {10.3390/proteomes6030029}, issn = {2227-7382}, year = {2018}, date = {2018-01-01}, journal = {Proteomes}, volume = {6}, number = {3}, abstract = {Epigenetic modifications contribute to the determination of cell fate and differentiation. The molecular mechanisms underlying histone variants and post-translational modifications (PTMs) have been studied in the contexts of development, differentiation, and disease. Antibody-based assays have classically been used to target PTMs, but these approaches fail to reveal combinatorial patterns of modifications. In addition, some histone variants are so similar to canonical histones that antibodies have difficulty distinguishing between these isoforms. Mass spectrometry (MS) has progressively developed as a powerful technology for the study of histone variants and their PTMs. Indeed, MS analyses highlighted exquisitely complex combinations of PTMs, suggesting “crosstalk” between them, and also revealed that PTM patterns are often variant-specific. Even though the sensitivity and acquisition speed of MS instruments have considerably increased alongside the development of computational tools for the study of multiple PTMs, it remains challenging to correctly describe the landscape of histone PTMs, and in particular to confidently assign modifications to specific amino acids. Here, we provide an inventory of MS-based strategies and of the pitfalls inherent to histone PTM and variant characterization, while stressing the complex interplay between PTMs and histone sequence variations. We will particularly illustrate the roles played by MS-based analyses in identifying and quantifying histone variants and modifications.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Epigenetic modifications contribute to the determination of cell fate and differentiation. The molecular mechanisms underlying histone variants and post-translational modifications (PTMs) have been studied in the contexts of development, differentiation, and disease. Antibody-based assays have classically been used to target PTMs, but these approaches fail to reveal combinatorial patterns of modifications. In addition, some histone variants are so similar to canonical histones that antibodies have difficulty distinguishing between these isoforms. Mass spectrometry (MS) has progressively developed as a powerful technology for the study of histone variants and their PTMs. Indeed, MS analyses highlighted exquisitely complex combinations of PTMs, suggesting “crosstalk” between them, and also revealed that PTM patterns are often variant-specific. Even though the sensitivity and acquisition speed of MS instruments have considerably increased alongside the development of computational tools for the study of multiple PTMs, it remains challenging to correctly describe the landscape of histone PTMs, and in particular to confidently assign modifications to specific amino acids. Here, we provide an inventory of MS-based strategies and of the pitfalls inherent to histone PTM and variant characterization, while stressing the complex interplay between PTMs and histone sequence variations. We will particularly illustrate the roles played by MS-based analyses in identifying and quantifying histone variants and modifications. |
Kennani, Sara El; Adrait, Annie; Permiakova, Olga; Hesse, Anne-Marie; Ialy-Radio, Côme; Ferro, Myriam; Brun, Virginie; Cocquet, Julie; Govin, Jérôme; Pflieger, Delphine Systematic quantitative analysis of Ħ2A and Ħ2B variants by targeted proteomics Article de journal Epigenetics & Chromatin, 11 (1), p. 2, 2018, ISSN: 1756-8935. @article{el_kennani_systematic_2018, title = {Systematic quantitative analysis of Ħ2A and Ħ2B variants by targeted proteomics}, author = {Sara El Kennani and Annie Adrait and Olga Permiakova and Anne-Marie Hesse and Côme Ialy-Radio and Myriam Ferro and Virginie Brun and Julie Cocquet and Jérôme Govin and Delphine Pflieger}, doi = {10.1186/s13072-017-0172-y}, issn = {1756-8935}, year = {2018}, date = {2018-01-01}, journal = {Epigenetics & Chromatin}, volume = {11}, number = {1}, pages = {2}, abstract = {BACKGROUND: Histones organize DNA into chromatin through a variety of processes. Among them, a vast diversity of histone variants can be incorporated into chromatin and finely modulate its organization and functionality. Classically, the study of histone variants has largely relied on antibody-based assays. However, antibodies have a limited efficiency to discriminate between highly similar histone variants. RESULTS: In this study, we established a mass spectrometry-based analysis to address this challenge. We developed a targeted proteomics method, using selected reaction monitoring or parallel reaction monitoring, to quantify a maximum number of histone variants in a single multiplexed assay, even when histones are present in a crude extract. This strategy was developed on H2A and H2B variants, using 55 peptides corresponding to 25 different histone sequences, among which a few differ by a single amino acid. The methodology was then applied to mouse testis extracts in which almost all histone variants are expressed. It confirmed the abundance profiles of several testis-specific histones during successive stages of spermatogenesis and the existence of predicted H2A.L.1 isoforms. This methodology was also used to explore the over-expression pattern of H2A.L.1 isoforms in a mouse model of male infertility. CONCLUSIONS: Our results demonstrate that targeted proteomics is a powerful method to quantify highly similar histone variants and isoforms. The developed method can be easily transposed to the study of human histone variants, whose abundance can be deregulated in various diseases.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BACKGROUND: Histones organize DNA into chromatin through a variety of processes. Among them, a vast diversity of histone variants can be incorporated into chromatin and finely modulate its organization and functionality. Classically, the study of histone variants has largely relied on antibody-based assays. However, antibodies have a limited efficiency to discriminate between highly similar histone variants. RESULTS: In this study, we established a mass spectrometry-based analysis to address this challenge. We developed a targeted proteomics method, using selected reaction monitoring or parallel reaction monitoring, to quantify a maximum number of histone variants in a single multiplexed assay, even when histones are present in a crude extract. This strategy was developed on H2A and H2B variants, using 55 peptides corresponding to 25 different histone sequences, among which a few differ by a single amino acid. The methodology was then applied to mouse testis extracts in which almost all histone variants are expressed. It confirmed the abundance profiles of several testis-specific histones during successive stages of spermatogenesis and the existence of predicted H2A.L.1 isoforms. This methodology was also used to explore the over-expression pattern of H2A.L.1 isoforms in a mouse model of male infertility. CONCLUSIONS: Our results demonstrate that targeted proteomics is a powerful method to quantify highly similar histone variants and isoforms. The developed method can be easily transposed to the study of human histone variants, whose abundance can be deregulated in various diseases. |
Adam, Céline; Guérois, Raphaël; Citarella, Anna; Verardi, Laura; Adolphe, Florine; Béneut, Claire; Sommermeyer, Vérane; Ramus, Claire; Govin, Jérôme; Couté, Yohann; Borde, Valérie The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes Article de journal PLoS genetics, 14 (2), p. e1007223, 2018, ISSN: 1553-7404. @article{adam_phd_2018, title = {The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes}, author = {Céline Adam and Raphaël Guérois and Anna Citarella and Laura Verardi and Florine Adolphe and Claire Béneut and Vérane Sommermeyer and Claire Ramus and Jérôme Govin and Yohann Couté and Valérie Borde}, doi = {10.1371/journal.pgen.1007223}, issn = {1553-7404}, year = {2018}, date = {2018-01-01}, journal = {PLoS genetics}, volume = {14}, number = {2}, pages = {e1007223}, abstract = {Histone H3K4 methylation is a feature of meiotic recombination hotspots shared by many organisms including plants and mammals. Meiotic recombination is initiated by programmed double-strand break (DSB) formation that in budding yeast takes place in gene promoters and is promoted by histone H3K4 di/trimethylation. This histone modification is recognized by Spp1, a PHD finger containing protein that belongs to the conserved histone H3K4 methyltransferase Set1 complex. During meiosis, Spp1 binds H3K4me3 and interacts with a DSB protein, Mer2, to promote DSB formation close to gene promoters. How Set1 complex- and Mer2- related functions of Spp1 are connected is not clear. Here, combining genome-wide localization analyses, biochemical approaches and the use of separation of function mutants, we show that Spp1 is present within two distinct complexes in meiotic cells, the Set1 and the Mer2 complexes. Disrupting the Spp1-Set1 interaction mildly decreases H3K4me3 levels and does not affect meiotic recombination initiation. Conversely, the Spp1-Mer2 interaction is required for normal meiotic recombination initiation, but dispensable for Set1 complex-mediated histone H3K4 methylation. Finally, we provide evidence that Spp1 preserves normal H3K4me3 levels independently of the Set1 complex. We propose a model where Spp1 works in three ways to promote recombination initiation: first by depositing histone H3K4 methylation (Set1 complex), next by "reading" and protecting histone H3K4 methylation, and finally by making the link with the chromosome axis (Mer2-Spp1 complex). This work deciphers the precise roles of Spp1 in meiotic recombination and opens perspectives to study its functions in other organisms where H3K4me3 is also present at recombination hotspots.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Histone H3K4 methylation is a feature of meiotic recombination hotspots shared by many organisms including plants and mammals. Meiotic recombination is initiated by programmed double-strand break (DSB) formation that in budding yeast takes place in gene promoters and is promoted by histone H3K4 di/trimethylation. This histone modification is recognized by Spp1, a PHD finger containing protein that belongs to the conserved histone H3K4 methyltransferase Set1 complex. During meiosis, Spp1 binds H3K4me3 and interacts with a DSB protein, Mer2, to promote DSB formation close to gene promoters. How Set1 complex- and Mer2- related functions of Spp1 are connected is not clear. Here, combining genome-wide localization analyses, biochemical approaches and the use of separation of function mutants, we show that Spp1 is present within two distinct complexes in meiotic cells, the Set1 and the Mer2 complexes. Disrupting the Spp1-Set1 interaction mildly decreases H3K4me3 levels and does not affect meiotic recombination initiation. Conversely, the Spp1-Mer2 interaction is required for normal meiotic recombination initiation, but dispensable for Set1 complex-mediated histone H3K4 methylation. Finally, we provide evidence that Spp1 preserves normal H3K4me3 levels independently of the Set1 complex. We propose a model where Spp1 works in three ways to promote recombination initiation: first by depositing histone H3K4 methylation (Set1 complex), next by "reading" and protecting histone H3K4 methylation, and finally by making the link with the chromosome axis (Mer2-Spp1 complex). This work deciphers the precise roles of Spp1 in meiotic recombination and opens perspectives to study its functions in other organisms where H3K4me3 is also present at recombination hotspots. |
Muyt, Arnaud De; Pyatnitskaya, Alexandra; Andréani, Jessica; Ranjha, Lepakshi; Ramus, Claire; Laureau, Raphaëlle; Fernandez-Vega, Ambra; Holoch, Daniel; Girard, Elodie; Govin, Jérome; Margueron, Raphaël; Couté, Yohann; Cejka, Petr; Guérois, Raphaël; Borde, Valérie A meiotic XPF-ERCC1-like complex recognizes joint molecule recombination intermediates to promote crossover formation Article de journal Genes & Development, 32 (3-4), p. 283–296, 2018, ISSN: 1549-5477. @article{de_muyt_meiotic_2018, title = {A meiotic XPF-ERCC1-like complex recognizes joint molecule recombination intermediates to promote crossover formation}, author = {Arnaud De Muyt and Alexandra Pyatnitskaya and Jessica Andréani and Lepakshi Ranjha and Claire Ramus and Raphaëlle Laureau and Ambra Fernandez-Vega and Daniel Holoch and Elodie Girard and Jérome Govin and Raphaël Margueron and Yohann Couté and Petr Cejka and Raphaël Guérois and Valérie Borde}, doi = {10.1101/gad.308510.117}, issn = {1549-5477}, year = {2018}, date = {2018-01-01}, journal = {Genes & Development}, volume = {32}, number = {3-4}, pages = {283--296}, abstract = {Meiotic crossover formation requires the stabilization of early recombination intermediates by a set of proteins and occurs within the environment of the chromosome axis, a structure important for the regulation of meiotic recombination events. The molecular mechanisms underlying and connecting crossover recombination and axis localization are elusive. Here, we identified the ZZS (Zip2-Zip4-Spo16) complex, required for crossover formation, which carries two distinct activities: one provided by Zip4, which acts as hub through physical interactions with components of the chromosome axis and the crossover machinery, and the other carried by Zip2 and Spo16, which preferentially bind branched DNA molecules in vitro. We found that Zip2 and Spo16 share structural similarities to the structure-specific XPF-ERCC1 nuclease, although it lacks endonuclease activity. The XPF domain of Zip2 is required for crossover formation, suggesting that, together with Spo16, it has a noncatalytic DNA recognition function. Our results suggest that the ZZS complex shepherds recombination intermediates toward crossovers as a dynamic structural module that connects recombination events to the chromosome axis. The identification of the ZZS complex improves our understanding of the various activities required for crossover implementation and is likely applicable to other organisms, including mammals.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Meiotic crossover formation requires the stabilization of early recombination intermediates by a set of proteins and occurs within the environment of the chromosome axis, a structure important for the regulation of meiotic recombination events. The molecular mechanisms underlying and connecting crossover recombination and axis localization are elusive. Here, we identified the ZZS (Zip2-Zip4-Spo16) complex, required for crossover formation, which carries two distinct activities: one provided by Zip4, which acts as hub through physical interactions with components of the chromosome axis and the crossover machinery, and the other carried by Zip2 and Spo16, which preferentially bind branched DNA molecules in vitro. We found that Zip2 and Spo16 share structural similarities to the structure-specific XPF-ERCC1 nuclease, although it lacks endonuclease activity. The XPF domain of Zip2 is required for crossover formation, suggesting that, together with Spo16, it has a noncatalytic DNA recognition function. Our results suggest that the ZZS complex shepherds recombination intermediates toward crossovers as a dynamic structural module that connects recombination events to the chromosome axis. The identification of the ZZS complex improves our understanding of the various activities required for crossover implementation and is likely applicable to other organisms, including mammals. |
Petosa, Carlo; Govin, Jérôme; Mietton, Flore [A new hope to fight invasive fungal infection] Article de journal Medecine Sciences: M/S, 34 (2), p. 123–125, 2018, ISSN: 1958-5381. @article{petosa_[new_2018, title = {[A new hope to fight invasive fungal infection]}, author = {Carlo Petosa and Jérôme Govin and Flore Mietton}, doi = {10.1051/medsci/20183402007}, issn = {1958-5381}, year = {2018}, date = {2018-01-01}, journal = {Medecine Sciences: M/S}, volume = {34}, number = {2}, pages = {123--125}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Garnaud, Cécile; García-Oliver, Encar; Wang, Yan; Maubon, Danièle; Bailly, Sébastien; Despinasse, Quentin; Champleboux, Morgane; Govin, Jérôme; Cornet, Muriel The Rim Pathway Mediates Antifungal Tolerance in Candida albicans through Newly Identified Rim101 Transcriptional Targets, Including Hsp90 and Ipt1 Article de journal Antimicrobial Agents and Chemotherapy, 62 (3), 2018, ISSN: 1098-6596. @article{garnaud_rim_2018, title = {The Rim Pathway Mediates Antifungal Tolerance in Candida albicans through Newly Identified Rim101 Transcriptional Targets, Including Hsp90 and Ipt1}, author = {Cécile Garnaud and Encar García-Oliver and Yan Wang and Danièle Maubon and Sébastien Bailly and Quentin Despinasse and Morgane Champleboux and Jérôme Govin and Muriel Cornet}, doi = {10.1128/AAC.01785-17}, issn = {1098-6596}, year = {2018}, date = {2018-01-01}, journal = {Antimicrobial Agents and Chemotherapy}, volume = {62}, number = {3}, abstract = {Invasive candidiasis (IC) is a major cause of morbidity and mortality despite antifungal treatment. Azoles and echinocandins are used as first-line therapies for IC. However, their efficacy is limited by yeast tolerance and the emergence of acquired resistance. Tolerance is a reversible stage created due to the yeast's capacity to counter antifungal drug exposure, leading to persistent growth. For Candida albicans, multiple stress signaling pathways have been shown to contribute to this adaptation. Among them, the pH-responsive Rim pathway, through its transcription factor Rim101p, was shown to mediate azole and echinocandin tolerance. The Rim pathway is fungus specific, is conserved among the members of the fungal kingdom, and plays a key role in pathogenesis and virulence. The present study aimed at confirming the role of Rim101p and investigating the implication of the other Rim proteins in antifungal tolerance in C. albicans, as well as the mechanisms underlying it. Time-kill curve experiments and colony formation tests showed that genetic inhibition of all the Rim factors enhances echinocandin and azole antifungal activity. Through RNA sequencing analysis of a rim101-/- mutant, a strain constitutively overexpressing RIM101, and control strains, we discovered novel Rim-dependent genes involved in tolerance, including HSP90, encoding a major molecular chaperone, and IPT1, involved in sphingolipid biosynthesis. Rim mutants were also hypersensitive to pharmacological inhibition of Hsp90. Taken together, these data suggest that Rim101 acts upstream of Hsp90 and that targeting the Rim pathway in combination with existing antifungal drugs may represent a promising antifungal strategy to indirectly but specifically target Hsp90 in yeasts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Invasive candidiasis (IC) is a major cause of morbidity and mortality despite antifungal treatment. Azoles and echinocandins are used as first-line therapies for IC. However, their efficacy is limited by yeast tolerance and the emergence of acquired resistance. Tolerance is a reversible stage created due to the yeast's capacity to counter antifungal drug exposure, leading to persistent growth. For Candida albicans, multiple stress signaling pathways have been shown to contribute to this adaptation. Among them, the pH-responsive Rim pathway, through its transcription factor Rim101p, was shown to mediate azole and echinocandin tolerance. The Rim pathway is fungus specific, is conserved among the members of the fungal kingdom, and plays a key role in pathogenesis and virulence. The present study aimed at confirming the role of Rim101p and investigating the implication of the other Rim proteins in antifungal tolerance in C. albicans, as well as the mechanisms underlying it. Time-kill curve experiments and colony formation tests showed that genetic inhibition of all the Rim factors enhances echinocandin and azole antifungal activity. Through RNA sequencing analysis of a rim101-/- mutant, a strain constitutively overexpressing RIM101, and control strains, we discovered novel Rim-dependent genes involved in tolerance, including HSP90, encoding a major molecular chaperone, and IPT1, involved in sphingolipid biosynthesis. Rim mutants were also hypersensitive to pharmacological inhibition of Hsp90. Taken together, these data suggest that Rim101 acts upstream of Hsp90 and that targeting the Rim pathway in combination with existing antifungal drugs may represent a promising antifungal strategy to indirectly but specifically target Hsp90 in yeasts. |
He, Huan; Brenier-Pinchart, Marie-Pierre; Braun, Laurence; Kraut, Alexandra; Touquet, Bastien; Couté, Yohann; Tardieux, Isabelle; Hakimi, Mohamed-Ali; Bougdour, Alexandre Characterization of a Toxoplasma effector uncovers an alternative GSK3/β-catenin-regulatory pathway of inflammation Article de journal eLife, 7 , 2018, ISSN: 2050-084X. @article{he_characterization_2018, title = {Characterization of a Toxoplasma effector uncovers an alternative GSK3/β-catenin-regulatory pathway of inflammation}, author = {Huan He and Marie-Pierre Brenier-Pinchart and Laurence Braun and Alexandra Kraut and Bastien Touquet and Yohann Couté and Isabelle Tardieux and Mohamed-Ali Hakimi and Alexandre Bougdour}, doi = {10.7554/eLife.39887}, issn = {2050-084X}, year = {2018}, date = {2018-01-01}, journal = {eLife}, volume = {7}, abstract = {The intracellular parasite Toxoplasma gondii, hijacks evolutionarily conserved host processes by delivering effector proteins into the host cell that shift gene expression in a timely fashion. We identified a parasite dense granule protein as GRA18 that once released in the host cell cytoplasm forms versatile complexes with regulatory elements of the β-catenin destruction complex. By interacting with GSK3/PP2A-B56, GRA18 drives β-catenin up-regulation and the downstream effects on host cell gene expression. In the context of macrophages infection, GRA18 induces the expression of a specific set of genes commonly associated with an anti-inflammatory response that includes those encoding chemokines CCL17 and CCL22. Overall, this study adds another original strategy by which T. gondii tachyzoites reshuffle the host cell interactome through a GSK3/β-catenin axis to selectively reprogram immune gene expression.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The intracellular parasite Toxoplasma gondii, hijacks evolutionarily conserved host processes by delivering effector proteins into the host cell that shift gene expression in a timely fashion. We identified a parasite dense granule protein as GRA18 that once released in the host cell cytoplasm forms versatile complexes with regulatory elements of the β-catenin destruction complex. By interacting with GSK3/PP2A-B56, GRA18 drives β-catenin up-regulation and the downstream effects on host cell gene expression. In the context of macrophages infection, GRA18 induces the expression of a specific set of genes commonly associated with an anti-inflammatory response that includes those encoding chemokines CCL17 and CCL22. Overall, this study adds another original strategy by which T. gondii tachyzoites reshuffle the host cell interactome through a GSK3/β-catenin axis to selectively reprogram immune gene expression. |
Picard, Marion A L; Cosseau, Celine; Ferré, Sabrina; Quack, Thomas; Grevelding, Christoph G; Couté, Yohann; Vicoso, Beatriz Evolution of gene dosage on the Z-chromosome of schistosome parasites Article de journal eLife, 7 , 2018, ISSN: 2050-084X. @article{picard_evolution_2018, title = {Evolution of gene dosage on the Z-chromosome of schistosome parasites}, author = {Marion A L Picard and Celine Cosseau and Sabrina Ferré and Thomas Quack and Christoph G Grevelding and Yohann Couté and Beatriz Vicoso}, doi = {10.7554/eLife.35684}, issn = {2050-084X}, year = {2018}, date = {2018-01-01}, journal = {eLife}, volume = {7}, abstract = {XY systems usually show chromosome-wide compensation of X-linked genes, while in many ZW systems, compensation is restricted to a minority of dosage-sensitive genes. Why such differences arose is still unclear. Here, we combine comparative genomics, transcriptomics and proteomics to obtain a complete overview of the evolution of gene dosage on the Z-chromosome of Schistosoma parasites. We compare the Z-chromosome gene content of African (Schistosoma mansoni and S. haematobium) and Asian (S. japonicum) schistosomes and describe lineage-specific evolutionary strata. We use these to assess gene expression evolution following sex-linkage. The resulting patterns suggest a reduction in expression of Z-linked genes in females, combined with upregulation of the Z in both sexes, in line with the first step of Ohno's classic model of dosage compensation evolution. Quantitative proteomics suggest that post-transcriptional mechanisms do not play a major role in balancing the expression of Z-linked genes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } XY systems usually show chromosome-wide compensation of X-linked genes, while in many ZW systems, compensation is restricted to a minority of dosage-sensitive genes. Why such differences arose is still unclear. Here, we combine comparative genomics, transcriptomics and proteomics to obtain a complete overview of the evolution of gene dosage on the Z-chromosome of Schistosoma parasites. We compare the Z-chromosome gene content of African (Schistosoma mansoni and S. haematobium) and Asian (S. japonicum) schistosomes and describe lineage-specific evolutionary strata. We use these to assess gene expression evolution following sex-linkage. The resulting patterns suggest a reduction in expression of Z-linked genes in females, combined with upregulation of the Z in both sexes, in line with the first step of Ohno's classic model of dosage compensation evolution. Quantitative proteomics suggest that post-transcriptional mechanisms do not play a major role in balancing the expression of Z-linked genes. |
Legendre, Matthieu; Fabre, Elisabeth; Poirot, Olivier; Jeudy, Sandra; Lartigue, Audrey; Alempic, Jean-Marie; Beucher, Laure; Philippe, Nadège; Bertaux, Lionel; Christo-Foroux, Eugène; Labadie, Karine; Couté, Yohann; Abergel, Chantal; Claverie, Jean-Michel Diversity and evolution of the emerging Pandoraviridae family Article de journal Nature Communications, 9 (1), p. 2285, 2018, ISSN: 2041-1723. @article{legendre_diversity_2018, title = {Diversity and evolution of the emerging Pandoraviridae family}, author = {Matthieu Legendre and Elisabeth Fabre and Olivier Poirot and Sandra Jeudy and Audrey Lartigue and Jean-Marie Alempic and Laure Beucher and Nadège Philippe and Lionel Bertaux and Eugène Christo-Foroux and Karine Labadie and Yohann Couté and Chantal Abergel and Jean-Michel Claverie}, doi = {10.1038/s41467-018-04698-4}, issn = {2041-1723}, year = {2018}, date = {2018-01-01}, journal = {Nature Communications}, volume = {9}, number = {1}, pages = {2285}, abstract = {With DNA genomes reaching 2.5 Mb packed in particles of bacterium-like shape and dimension, the first two Acanthamoeba-infecting pandoraviruses remained up to now the most complex viruses since their discovery in 2013. Our isolation of three new strains from distant locations and environments is now used to perform the first comparative genomics analysis of the emerging worldwide-distributed Pandoraviridae family. Thorough annotation of the genomes combining transcriptomic, proteomic, and bioinformatic analyses reveals many non-coding transcripts and significantly reduces the former set of predicted protein-coding genes. Here we show that the pandoraviruses exhibit an open pan-genome, the enormous size of which is not adequately explained by gene duplications or horizontal transfers. As most of the strain-specific genes have no extant homolog and exhibit statistical features comparable to intergenic regions, we suggest that de novo gene creation could contribute to the evolution of the giant pandoravirus genomes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } With DNA genomes reaching 2.5 Mb packed in particles of bacterium-like shape and dimension, the first two Acanthamoeba-infecting pandoraviruses remained up to now the most complex viruses since their discovery in 2013. Our isolation of three new strains from distant locations and environments is now used to perform the first comparative genomics analysis of the emerging worldwide-distributed Pandoraviridae family. Thorough annotation of the genomes combining transcriptomic, proteomic, and bioinformatic analyses reveals many non-coding transcripts and significantly reduces the former set of predicted protein-coding genes. Here we show that the pandoraviruses exhibit an open pan-genome, the enormous size of which is not adequately explained by gene duplications or horizontal transfers. As most of the strain-specific genes have no extant homolog and exhibit statistical features comparable to intergenic regions, we suggest that de novo gene creation could contribute to the evolution of the giant pandoravirus genomes. |
Eymard-Vernain, Elise; Couté, Yohann; Adrait, Annie; Rabilloud, Thierry; Sarret, Géraldine; Lelong, Cécile The poly-gamma-glutamate of Bacillus subtilis interacts specifically with silver nanoparticles Article de journal PloS One, 13 (5), p. e0197501, 2018, ISSN: 1932-6203. @article{eymard-vernain_poly-gamma-glutamate_2018, title = {The poly-gamma-glutamate of Bacillus subtilis interacts specifically with silver nanoparticles}, author = {Elise Eymard-Vernain and Yohann Couté and Annie Adrait and Thierry Rabilloud and Géraldine Sarret and Cécile Lelong}, doi = {10.1371/journal.pone.0197501}, issn = {1932-6203}, year = {2018}, date = {2018-01-01}, journal = {PloS One}, volume = {13}, number = {5}, pages = {e0197501}, abstract = {For many years, silver nanoparticles, as with other antibacterial nanoparticles, have been extensively used in manufactured products. However, their fate in the environment is unclear and raises questions. We studied the fate of silver nanoparticles in the presence of bacteria under growth conditions that are similar to those found naturally in the environment (that is, bacteria in a stationary phase with low nutrient concentrations). We demonstrated that the viability and the metabolism of a gram-positive bacteria, Bacillus subtilis, exposed during the stationary phase is unaffected by 1 mg/L of silver nanoparticles. These results can be partly explained by a physical interaction of the poly-gamma-glutamate (PGA) secreted by Bacillus subtilis with the silver nanoparticles. The coating of the silver nanoparticles by the secreted PGA likely results in a loss of the bioavailability of nanoparticles and, consequently, a decrease of their biocidal effect.}, keywords = {}, pubstate = {published}, tppubtype = {article} } For many years, silver nanoparticles, as with other antibacterial nanoparticles, have been extensively used in manufactured products. However, their fate in the environment is unclear and raises questions. We studied the fate of silver nanoparticles in the presence of bacteria under growth conditions that are similar to those found naturally in the environment (that is, bacteria in a stationary phase with low nutrient concentrations). We demonstrated that the viability and the metabolism of a gram-positive bacteria, Bacillus subtilis, exposed during the stationary phase is unaffected by 1 mg/L of silver nanoparticles. These results can be partly explained by a physical interaction of the poly-gamma-glutamate (PGA) secreted by Bacillus subtilis with the silver nanoparticles. The coating of the silver nanoparticles by the secreted PGA likely results in a loss of the bioavailability of nanoparticles and, consequently, a decrease of their biocidal effect. |
Abulfaraj, Aala A; Mariappan, Kiruthiga; Bigeard, Jean; Manickam, Prabhu; Blilou, Ikram; Guo, Xiujie; Al-Babili, Salim; Pflieger, Delphine; Hirt, Heribert; Rayapuram, Naganand The Arabidopsis homolog of human G3BP1 is a key regulator of stomatal and apoplastic immunity Article de journal Life Science Alliance, 1 (2), p. e201800046, 2018, ISSN: 2575-1077. @article{abulfaraj_arabidopsis_2018, title = {The Arabidopsis homolog of human G3BP1 is a key regulator of stomatal and apoplastic immunity}, author = {Aala A Abulfaraj and Kiruthiga Mariappan and Jean Bigeard and Prabhu Manickam and Ikram Blilou and Xiujie Guo and Salim Al-Babili and Delphine Pflieger and Heribert Hirt and Naganand Rayapuram}, doi = {10.26508/lsa.201800046}, issn = {2575-1077}, year = {2018}, date = {2018-01-01}, journal = {Life Science Alliance}, volume = {1}, number = {2}, pages = {e201800046}, abstract = {Mammalian Ras-GTPase-activating protein SH3-domain-binding proteins (G3BPs) are a highly conserved family of RNA-binding proteins that link kinase receptor-mediated signaling to RNA metabolism. Mammalian G3BP1 is a multifunctional protein that functions in viral immunity. Here, we show that the Arabidopsis thaliana homolog of human G3BP1 negatively regulates plant immunity. Arabidopsis g3bp1 mutants showed enhanced resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato. Pathogen resistance was mediated in Atg3bp1 mutants by altered stomatal and apoplastic immunity. Atg3bp1 mutants restricted pathogen entry into stomates showing insensitivity to bacterial coronatine-mediated stomatal reopening. AtG3BP1 was identified as a negative regulator of defense responses, which correlated with moderate up-regulation of salicylic acid biosynthesis and signaling without growth penalty.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Mammalian Ras-GTPase-activating protein SH3-domain-binding proteins (G3BPs) are a highly conserved family of RNA-binding proteins that link kinase receptor-mediated signaling to RNA metabolism. Mammalian G3BP1 is a multifunctional protein that functions in viral immunity. Here, we show that the Arabidopsis thaliana homolog of human G3BP1 negatively regulates plant immunity. Arabidopsis g3bp1 mutants showed enhanced resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato. Pathogen resistance was mediated in Atg3bp1 mutants by altered stomatal and apoplastic immunity. Atg3bp1 mutants restricted pathogen entry into stomates showing insensitivity to bacterial coronatine-mediated stomatal reopening. AtG3BP1 was identified as a negative regulator of defense responses, which correlated with moderate up-regulation of salicylic acid biosynthesis and signaling without growth penalty. |
Sage, Eric; Sansa, Marc; Fostner, Shawn; Defoort, Martial; Gély, Marc; Naik, Akshay K; Morel, Robert; Duraffourg, Laurent; Roukes, Michael L; Alava, Thomas; Jourdan, Guillaume; Colinet, Eric; Masselon, Christophe; Brenac, Ariel; Hentz, Sébastien Single-particle mass spectrometry with arrays of frequency-addressed nanomechanical resonators Article de journal Nature Communications, 9 (1), p. 3283, 2018, ISSN: 2041-1723. @article{sage_single-particle_2018, title = {Single-particle mass spectrometry with arrays of frequency-addressed nanomechanical resonators}, author = {Eric Sage and Marc Sansa and Shawn Fostner and Martial Defoort and Marc Gély and Akshay K Naik and Robert Morel and Laurent Duraffourg and Michael L Roukes and Thomas Alava and Guillaume Jourdan and Eric Colinet and Christophe Masselon and Ariel Brenac and Sébastien Hentz}, doi = {10.1038/s41467-018-05783-4}, issn = {2041-1723}, year = {2018}, date = {2018-01-01}, journal = {Nature Communications}, volume = {9}, number = {1}, pages = {3283}, abstract = {One of the main challenges to overcome to perform nanomechanical mass spectrometry analysis in a practical time frame stems from the size mismatch between the analyte beam and the small nanomechanical detector area. We report here the demonstration of mass spectrometry with arrays of 20 multiplexed nanomechanical resonators; each resonator is designed with a distinct resonance frequency which becomes its individual address. Mass spectra of metallic aggregates in the MDa range are acquired with more than one order of magnitude improvement in analysis time compared to individual resonators. A 20 NEMS array is probed in 150 ms with the same mass limit of detection as a single resonator. Spectra acquired with a conventional time-of-flight mass spectrometer in the same system show excellent agreement. We also demonstrate how mass spectrometry imaging at the single-particle level becomes possible by mapping a 4-cm-particle beam in the MDa range and above.}, keywords = {}, pubstate = {published}, tppubtype = {article} } One of the main challenges to overcome to perform nanomechanical mass spectrometry analysis in a practical time frame stems from the size mismatch between the analyte beam and the small nanomechanical detector area. We report here the demonstration of mass spectrometry with arrays of 20 multiplexed nanomechanical resonators; each resonator is designed with a distinct resonance frequency which becomes its individual address. Mass spectra of metallic aggregates in the MDa range are acquired with more than one order of magnitude improvement in analysis time compared to individual resonators. A 20 NEMS array is probed in 150 ms with the same mass limit of detection as a single resonator. Spectra acquired with a conventional time-of-flight mass spectrometer in the same system show excellent agreement. We also demonstrate how mass spectrometry imaging at the single-particle level becomes possible by mapping a 4-cm-particle beam in the MDa range and above. |
Berthier, A; Vinod, M; Porez, G; Steenackers, A; Alexandre, J; Yamakawa, N; Gheeraert, C; Ploton, M; Maréchal, X; Dubois-Chevalier, J; Hovasse, A; Schaeffer-Reiss, C; Cianférani, S; Rolando, C; Bray, F; Duez, H; Eeckhoute, J; Lefebvre, T; Staels, B; Lefebvre, P Combinatorial regulation of hepatic cytoplasmic signaling and nuclear transcriptional events by the OGT/REV-ERBα complex. Article de journal Proc Natl Acad Sci U S A., 15 (47), p. E11033-E11042., 2018. @article{621, title = {Combinatorial regulation of hepatic cytoplasmic signaling and nuclear transcriptional events by the OGT/REV-ERBα complex.}, author = {A Berthier and M Vinod and G Porez and A Steenackers and J Alexandre and N Yamakawa and C Gheeraert and M Ploton and X Maréchal and J Dubois-Chevalier and A Hovasse and C Schaeffer-Reiss and S Cianférani and C Rolando and F Bray and H Duez and J Eeckhoute and T Lefebvre and B Staels and P Lefebvre}, doi = {10.1073/pnas.1805397115}, year = {2018}, date = {2018-01-01}, journal = {Proc Natl Acad Sci U S A.}, volume = {15}, number = {47}, pages = {E11033-E11042.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Bibi-Triki, S; Husson, G; Maucourt, B; Vuilleumier, S; Carapito, C; Bringel, F N-terminome and proteogenomic analysis of the Methylobacterium extorquens DM4 reference strain for dichloromethane utilization. Article de journal J Proteomics., (179), p. 131-139, 2018. @article{606, title = {N-terminome and proteogenomic analysis of the Methylobacterium extorquens DM4 reference strain for dichloromethane utilization.}, author = {S Bibi-Triki and G Husson and B Maucourt and S Vuilleumier and C Carapito and F Bringel}, year = {2018}, date = {2018-01-01}, journal = {J Proteomics.}, number = {179}, pages = {131-139}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Boeuf, A; Schnell, G; Bernard, Q; Kern, A; Westermann, B; Ehret-Sabatier, L; Grillon, A; Schramm, F; Jaulhac, B; Boulanger, N Dissociating effect of salivary gland extract from Ixodes ricinus on human fibroblasts: Potential impact on Borrelia transmission. Article de journal Ticks Tick Borne Dis., pii: S1877-959X(18)30014-1. , 2018, (2012/29). @article{626, title = {Dissociating effect of salivary gland extract from Ixodes ricinus on human fibroblasts: Potential impact on Borrelia transmission.}, author = {A Boeuf and G Schnell and Q Bernard and A Kern and B Westermann and L Ehret-Sabatier and A Grillon and F Schramm and B Jaulhac and N Boulanger}, year = {2018}, date = {2018-01-01}, journal = {Ticks Tick Borne Dis.}, volume = {pii: S1877-959X(18)30014-1.}, note = {2012/29}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Carapito, R; Carapito, C; Morlon, A; Paul, N; Jacome, AS. Vaca; Alsaleh, G; Rolli, V; Tahar, O; Aouadi, I; Rompais, M; Delalande, F; Pichot, A; Georgel, P; Messer, L; Sibilia, J; Cianferani, S; Dorsselaer, Van A; Bahram, S Multi-OMICS analyses unveil STAT1 as a potential modifier gene in mevalonate kinase deficiency. Article de journal Ann Rheum Dis., 77 (11), p. 1675-1687., 2018. @article{616, title = {Multi-OMICS analyses unveil STAT1 as a potential modifier gene in mevalonate kinase deficiency.}, author = {R Carapito and C Carapito and A Morlon and N Paul and AS. Vaca Jacome and G Alsaleh and V Rolli and O Tahar and I Aouadi and M Rompais and F Delalande and A Pichot and P Georgel and L Messer and J Sibilia and S Cianferani and A Van Dorsselaer and S Bahram}, year = {2018}, date = {2018-01-01}, journal = {Ann Rheum Dis.}, volume = {77}, number = {11}, pages = {1675-1687.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cavallo, G; Poyer, S; Amalian, JA.; Dufour, F; Burel, A; Carapito, C; Charles, L; Lutz, JF. Cleavable Binary Dyads: Simplifying Data Extraction and Increasing Storage Density in Digital Polymers. Article de journal Angew Chem Int Ed Engl., 57 (21), p. 6266-6269., 2018. @article{605, title = {Cleavable Binary Dyads: Simplifying Data Extraction and Increasing Storage Density in Digital Polymers.}, author = {G Cavallo and S Poyer and JA. Amalian and F Dufour and A Burel and C Carapito and L Charles and JF. Lutz}, year = {2018}, date = {2018-01-01}, journal = {Angew Chem Int Ed Engl.}, volume = {57}, number = {21}, pages = {6266-6269.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
B, Toubhans Durand Chery Robert Vieille-Marchiset Swenson JE Zedrosser Evans AL Brunberg Arnemo JM Gauquelin-Koch Storey KB Simon Blanc Bertile Lefai Chanon Chazarin B C I M A A S G C S F E S Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells Article de journal Sci Rep 2018, 8 (1), p. 5525, 2018, (2011/34). @article{597, title = {Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells}, author = {Toubhans Durand Chery Robert Vieille-Marchiset Swenson JE Zedrosser Evans AL Brunberg Arnemo JM Gauquelin-Koch Storey KB Simon Blanc Bertile Lefai B C I M A A S G C S F E Chanon S Chazarin B}, year = {2018}, date = {2018-01-01}, journal = {Sci Rep 2018}, volume = {8}, number = {1}, pages = {5525}, note = {2011/34}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chopard, C; Tong, PBV.; Tóth, P; Schatz, M; Yezid, H; Debaisieux, S; Mettling, C; Gross, A; Pugnière, M; Tu, A; Strub, JM.; Mesnard, JM.; Vitale, N; Beaumelle, B Cyclophilin A enables specific HIV-1 Tat palmitoylation and accumulation in uninfected cells. Article de journal Nat Commun., 2018, (2006/01). @article{608, title = {Cyclophilin A enables specific HIV-1 Tat palmitoylation and accumulation in uninfected cells.}, author = {C Chopard and PBV. Tong and P Tóth and M Schatz and H Yezid and S Debaisieux and C Mettling and A Gross and M Pugnière and A Tu and JM. Strub and JM. Mesnard and N Vitale and B Beaumelle}, year = {2018}, date = {2018-01-01}, journal = {Nat Commun.}, note = {2006/01}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Coulter, ME.; Dorobantu, CM.; Lodewijk, GA.; Delalande, F; Cianferani, S; Ganesh, VS.; Smith, RS.; Lim, ET.; Xu, CS.; Pang, S; Wong, ET.; Lidov, HGW.; Calicchio, ML.; Yang, E; Gonzalez, DM.; Schlaeger, TM.; Mochida, GH.; Hess, H; Lee, WA.; Lehtinen, MK.; Kirchhausen, T; Haussler, D; Jacobs, FMJ.; Gaudin, R; Walsh, CA. The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles. Article de journal Cell Rep., 24 (4), p. 973-986.e8., 2018. @article{614, title = {The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.}, author = {ME. Coulter and CM. Dorobantu and GA. Lodewijk and F Delalande and S Cianferani and VS. Ganesh and RS. Smith and ET. Lim and CS. Xu and S Pang and ET. Wong and HGW. Lidov and ML. Calicchio and E Yang and DM. Gonzalez and TM. Schlaeger and GH. Mochida and H Hess and WA. Lee and MK. Lehtinen and T Kirchhausen and D Haussler and FMJ. Jacobs and R Gaudin and CA. Walsh}, year = {2018}, date = {2018-01-01}, journal = {Cell Rep.}, volume = {24}, number = {4}, pages = {973-986.e8.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Criscuolo, F; Sorci, G; Behaim-Delarbre, M; Zahn, S; Faivre, B; Bertile, F Age-related response to an acute innate immune challenge in mice: proteomics reveals a telomere maintenance-related cost. Article de journal Proceedings of the Royal Society B, 285 , p. 20181877., 2018, (2012/28). @article{622, title = {Age-related response to an acute innate immune challenge in mice: proteomics reveals a telomere maintenance-related cost.}, author = {F Criscuolo and G Sorci and M Behaim-Delarbre and S Zahn and B Faivre and F Bertile}, year = {2018}, date = {2018-01-01}, journal = {Proceedings of the Royal Society B}, volume = {285}, pages = {20181877.}, note = {2012/28}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dartevelle, P; Ehlinger, C; Zaet, A; Boehler, C; Rabineau, M; Westermann, B; Strub, JM.; Cianferani, S; Haïkel, Y; Metz-Boutigue, MH.; Marban, C D-Cateslytin: a new antifungal agent for the treatment of oral Candida albicans associated infections. Article de journal Sci Rep., 8 (1), p. 9235., 2018, (2009/25). @article{600, title = {D-Cateslytin: a new antifungal agent for the treatment of oral Candida albicans associated infections.}, author = {P Dartevelle and C Ehlinger and A Zaet and C Boehler and M Rabineau and B Westermann and JM. Strub and S Cianferani and Y Haïkel and MH. Metz-Boutigue and C Marban}, year = {2018}, date = {2018-01-01}, journal = {Sci Rep.}, volume = {8}, number = {1}, pages = {9235.}, note = {2009/25}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
D'Atri, V; Causon, T; Hernandez-Alba, O; Mutabazi, A; Veuthey, JL.; Cianferani, S; Guillarme, D Adding a new separation dimension to MS and LC-MS: What is the utility of ion mobility spectrometry? Article de journal J Sep Sci., 41 (1), p. 20-67., 2018, (0 (review)). @article{582, title = {Adding a new separation dimension to MS and LC-MS: What is the utility of ion mobility spectrometry?}, author = {V D'Atri and T Causon and O Hernandez-Alba and A Mutabazi and JL. Veuthey and S Cianferani and D Guillarme}, year = {2018}, date = {2018-01-01}, journal = {J Sep Sci.}, volume = {41}, number = {1}, pages = {20-67.}, note = {0 (review)}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
