ProFi Achievements
Publications
2018 |
Lacombe, Maud; Marie-Desvergne, Caroline; Combes, Florence; Kraut, Alexandra; Bruley, Christophe; Vandenbrouck, Yves; Mossuz, Véronique Chamel; Couté, Yohann; Brun, Virginie Proteomic characterization of human exhaled breath condensate Article de journal Journal of Breath Research, 12 (2), p. 021001, 2018, ISSN: 1752-7163. @article{lacombe_proteomic_2018, title = {Proteomic characterization of human exhaled breath condensate}, author = {Maud Lacombe and Caroline Marie-Desvergne and Florence Combes and Alexandra Kraut and Christophe Bruley and Yves Vandenbrouck and Véronique Chamel Mossuz and Yohann Couté and Virginie Brun}, doi = {10.1088/1752-7163/aa9e71}, issn = {1752-7163}, year = {2018}, date = {2018-02-01}, journal = {Journal of Breath Research}, volume = {12}, number = {2}, pages = {021001}, abstract = {To improve biomedical knowledge and to support biomarker discovery studies, it is essential to establish comprehensive proteome maps for human tissues and biofluids, and to make them publicly accessible. In this study, we performed an in-depth proteomics characterization of exhaled breath condensate (EBC), a sample obtained non-invasively by condensation of exhaled air that contains submicron droplets of airway lining fluid. Two pooled samples of EBC, each obtained from 10 healthy donors, were processed using a straightforward protocol based on sample lyophilization, in-gel digestion and liquid chromatography tandem-mass spectrometry analysis. Two 'technical' control samples were processed in parallel to the pooled samples to correct for exogenous protein contamination. A total of 229 unique proteins were identified in EBC among which 153 proteins were detected in both EBC pooled samples. A detailed bioinformatics analysis of these 153 proteins showed that most of the proteins identified corresponded to proteins secreted in the respiratory tract (lung, bronchi). Eight proteins were salivary proteins. Our dataset is described and has been made accessible through the ProteomeXchange database (dataset identifier: PXD007591) and is expected to be useful for future MS-based biomarker studies using EBC as the diagnostic specimen.}, keywords = {}, pubstate = {published}, tppubtype = {article} } To improve biomedical knowledge and to support biomarker discovery studies, it is essential to establish comprehensive proteome maps for human tissues and biofluids, and to make them publicly accessible. In this study, we performed an in-depth proteomics characterization of exhaled breath condensate (EBC), a sample obtained non-invasively by condensation of exhaled air that contains submicron droplets of airway lining fluid. Two pooled samples of EBC, each obtained from 10 healthy donors, were processed using a straightforward protocol based on sample lyophilization, in-gel digestion and liquid chromatography tandem-mass spectrometry analysis. Two 'technical' control samples were processed in parallel to the pooled samples to correct for exogenous protein contamination. A total of 229 unique proteins were identified in EBC among which 153 proteins were detected in both EBC pooled samples. A detailed bioinformatics analysis of these 153 proteins showed that most of the proteins identified corresponded to proteins secreted in the respiratory tract (lung, bronchi). Eight proteins were salivary proteins. Our dataset is described and has been made accessible through the ProteomeXchange database (dataset identifier: PXD007591) and is expected to be useful for future MS-based biomarker studies using EBC as the diagnostic specimen. |
Grzela, R; Nusbaum, J; Fieulaine, S; Lavecchia, F; Desmadril, M; Nhiri, N; Van Dorsselaer, A; Cianférani, S; Jacquet, E; Meinnel, T; Giglione, C Peptide deformylases from Vibrio parahaemolyticus phage and bacteria display similar deformylase activity and inhibitor binding clefts Article de journal Biochim Biophys Acta., 1866 (2), p. 348-355, 2018. @article{RN1310, title = {Peptide deformylases from Vibrio parahaemolyticus phage and bacteria display similar deformylase activity and inhibitor binding clefts}, author = {R Grzela and J Nusbaum and S Fieulaine and F Lavecchia and M Desmadril and N Nhiri and A {Van Dorsselaer} and S Cianférani and E Jacquet and T Meinnel and C Giglione}, doi = {10.1016/j.bbapap.2017.10.007}, year = {2018}, date = {2018-02-01}, journal = {Biochim Biophys Acta.}, volume = {1866}, number = {2}, pages = {348-355}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
A, Métais; I, Lamsoul; A, Melet; S, Uttenweiler-Joseph; R, Poincloux; S, Stefanovic; A, Valière; de A, Gonzalez Peredo; A, Stella; O, Burlet-Schiltz; S, Zaffran; PG, Lutz; C, Moog-Lutz Asb2α-Filamin A Axis Is Essential for Actin Cytoskeleton Remodeling During Heart Development Article de journal Circ Res., 122 (6), p. 34-48, 2018. @article{A2018, title = {Asb2α-Filamin A Axis Is Essential for Actin Cytoskeleton Remodeling During Heart Development}, author = {Métais A and Lamsoul I and Melet A and Uttenweiler-Joseph S and Poincloux R and Stefanovic S and Valière A and Gonzalez de Peredo A and Stella A and Burlet-Schiltz O and Zaffran S and Lutz PG and Moog-Lutz C}, doi = {10.1161/CIRCRESAHA.117.312015}, year = {2018}, date = {2018-01-26}, journal = {Circ Res.}, volume = {122}, number = {6}, pages = {34-48}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mori, M; Kovalenko, L; Malancona, S; Saladini, F; Forni, De D; Pires, M; Humbert, N; Real, E; Botzanowski, T; Cianférani, S; Giannini, A; Lang, MC. Dasso; Cugia, G; Poddesu, B; Lori, F; Zazzi, M; Harper, S; Summa, V; Mely, Y; Botta, M Structure-Based Identification of HIV-1 Nucleocapsid Protein Inhibitors Active against Wild-Type and Drug-Resistant HIV-1 Strains Article de journal ACS Chem Biol., 13 (1), p. 253-266, 2018. @article{RN1308, title = {Structure-Based Identification of HIV-1 Nucleocapsid Protein Inhibitors Active against Wild-Type and Drug-Resistant HIV-1 Strains}, author = {M Mori and L Kovalenko and S Malancona and F Saladini and D De Forni and M Pires and N Humbert and E Real and T Botzanowski and S Cianférani and A Giannini and MC. Dasso Lang and G Cugia and B Poddesu and F Lori and M Zazzi and S Harper and V Summa and Y Mely and M Botta}, doi = {10.1021/acschembio.7b00907}, year = {2018}, date = {2018-01-19}, journal = {ACS Chem Biol.}, volume = {13}, number = {1}, pages = {253-266}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Husson, G; Delangle, A; O'Hara, J; Cianférani, S; Gervais, A; Van Dorsselaer, A; Bracewell, D; Carapito, C Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development Article de journal Anal Chem., 90 (2), p. 1241-1247, 2018. @article{RN1306, title = {Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development}, author = {G Husson and A Delangle and J O'Hara and S Cianférani and A Gervais and A {Van Dorsselaer} and D Bracewell and C Carapito}, doi = {10.1021/acs.analchem.7b03965 }, year = {2018}, date = {2018-01-16}, journal = {Anal Chem.}, volume = {90}, number = {2}, pages = {1241-1247}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
L, Favre; A, Ortalo-Magné; C, Pichereaux; A, Gargaros; O, Burlet-Schiltz; V, Cotelle; G., Culioli Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8 Article de journal Biofouling, 34 (2), p. 132-148, 2018. @article{L2018, title = {Metabolome and proteome changes between biofilm and planktonic phenotypes of the marine bacterium Pseudoalteromonas lipolytica TC8}, author = {Favre L and Ortalo-Magné A and Pichereaux C and Gargaros A and Burlet-Schiltz O and Cotelle V and Culioli G.}, doi = {10.1080/08927014.2017.1413551}, year = {2018}, date = {2018-01-10}, journal = {Biofouling}, volume = {34}, number = {2}, pages = {132-148}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rayapuram, Naganand; Bigeard, Jean; Alhoraibi, Hanna; Bonhomme, Ludovic; Hesse, Anne-Marie; Vinh, Joëlle; Hirt, Heribert; Pflieger, Delphine Quantitative Phosphoproteomic Analysis Reveals Shared and Specific Targets ofArabidopsisMitogen-Activated Protein Kinases (MAPKs) MPK3, MPK4, and MPK6 Article de journal Molecular & cellular proteomics: MCP, 17 (1), p. 61–80, 2018, ISSN: 1535-9484. @article{rayapuram_quantitative_2018, title = {Quantitative Phosphoproteomic Analysis Reveals Shared and Specific Targets ofArabidopsisMitogen-Activated Protein Kinases (MAPKs) MPK3, MPK4, and MPK6}, author = {Naganand Rayapuram and Jean Bigeard and Hanna Alhoraibi and Ludovic Bonhomme and Anne-Marie Hesse and Joëlle Vinh and Heribert Hirt and Delphine Pflieger}, doi = {10.1074/mcp.RA117.000135}, issn = {1535-9484}, year = {2018}, date = {2018-01-01}, journal = {Molecular & cellular proteomics: MCP}, volume = {17}, number = {1}, pages = {61--80}, abstract = {In Arabidopsis, mitogen-activated protein kinases MPK3, MPK4, and MPK6 constitute essential relays for a variety of functions including cell division, development and innate immunity. Although some substrates of MPK3, MPK4 and MPK6 have been identified, the picture is still far from complete. To identify substrates of these MAPKs likely involved in cell division, growth and development we compared the phosphoproteomes of wild-type andmpk3,mpk4, andmpk6.To study the function of these MAPKs in innate immunity, we analyzed their phosphoproteomes following microbe-associated molecular pattern (MAMP) treatment. Partially overlapping substrates were retrieved for all three MAPKs, showing target specificity to one, two or all three MAPKs in different biological processes. More precisely, our results illustrate the fact that the entity to be defined as a specific or a shared substrate for MAPKs is not a phosphoprotein but a particular (S/T)P phosphorylation site in a given protein. One hundred fifty-two peptides were identified to be differentially phosphorylated in response to MAMP treatment and/or when compared between genotypes and 70 of them could be classified as putative MAPK targets. Biochemical analysis of a number of putative MAPK substrates by phosphorylation and interaction assays confirmed the global phosphoproteome approach. Our study also expands the set of MAPK substrates to involve other protein kinases, including calcium-dependent (CDPK) and sugar nonfermenting (SnRK) protein kinases.}, keywords = {}, pubstate = {published}, tppubtype = {article} } In Arabidopsis, mitogen-activated protein kinases MPK3, MPK4, and MPK6 constitute essential relays for a variety of functions including cell division, development and innate immunity. Although some substrates of MPK3, MPK4 and MPK6 have been identified, the picture is still far from complete. To identify substrates of these MAPKs likely involved in cell division, growth and development we compared the phosphoproteomes of wild-type andmpk3,mpk4, andmpk6.To study the function of these MAPKs in innate immunity, we analyzed their phosphoproteomes following microbe-associated molecular pattern (MAMP) treatment. Partially overlapping substrates were retrieved for all three MAPKs, showing target specificity to one, two or all three MAPKs in different biological processes. More precisely, our results illustrate the fact that the entity to be defined as a specific or a shared substrate for MAPKs is not a phosphoprotein but a particular (S/T)P phosphorylation site in a given protein. One hundred fifty-two peptides were identified to be differentially phosphorylated in response to MAMP treatment and/or when compared between genotypes and 70 of them could be classified as putative MAPK targets. Biochemical analysis of a number of putative MAPK substrates by phosphorylation and interaction assays confirmed the global phosphoproteome approach. Our study also expands the set of MAPK substrates to involve other protein kinases, including calcium-dependent (CDPK) and sugar nonfermenting (SnRK) protein kinases. |
Burger, Thomas Gentle Introduction to the Statistical Foundations of False Discovery Rate in Quantitative Proteomics Article de journal Journal of Proteome Research, 17 (1), p. 12–22, 2018, ISSN: 1535-3907. @article{burger_gentle_2018, title = {Gentle Introduction to the Statistical Foundations of False Discovery Rate in Quantitative Proteomics}, author = {Thomas Burger}, doi = {10.1021/acs.jproteome.7b00170}, issn = {1535-3907}, year = {2018}, date = {2018-01-01}, journal = {Journal of Proteome Research}, volume = {17}, number = {1}, pages = {12--22}, abstract = {The vocabulary of theoretical statistics can be difficult to embrace from the viewpoint of computational proteomics research, even though the notions it conveys are essential to publication guidelines. For example, "adjusted p-values", "q-values", and "false discovery rates" are essentially similar concepts, whereas "false discovery rate" and "false discovery proportion" must not be confused, even though "rate" and "proportion" are related in everyday language. In the interdisciplinary context of proteomics, such subtleties may cause misunderstandings. This article aims to provide an easy-to-understand explanation of these four notions (and a few other related ones). Their statistical foundations are dealt with from a perspective that largely relies on intuition, addressing mainly protein quantification but also, to some extent, peptide identification. In addition, a clear distinction is made between concepts that define an individual property (i.e., related to a peptide or a protein) and those that define a set property (i.e., related to a list of peptides or proteins).}, keywords = {}, pubstate = {published}, tppubtype = {article} } The vocabulary of theoretical statistics can be difficult to embrace from the viewpoint of computational proteomics research, even though the notions it conveys are essential to publication guidelines. For example, "adjusted p-values", "q-values", and "false discovery rates" are essentially similar concepts, whereas "false discovery rate" and "false discovery proportion" must not be confused, even though "rate" and "proportion" are related in everyday language. In the interdisciplinary context of proteomics, such subtleties may cause misunderstandings. This article aims to provide an easy-to-understand explanation of these four notions (and a few other related ones). Their statistical foundations are dealt with from a perspective that largely relies on intuition, addressing mainly protein quantification but also, to some extent, peptide identification. In addition, a clear distinction is made between concepts that define an individual property (i.e., related to a peptide or a protein) and those that define a set property (i.e., related to a list of peptides or proteins). |
Kennani, Sara El; Crespo, Marion; Govin, Jérôme; Pflieger, Delphine Proteomic Analysis of Histone Variants and Their PTMs: Strategies and Pitfalls Article de journal Proteomes, 6 (3), 2018, ISSN: 2227-7382. @article{el_kennani_proteomic_2018, title = {Proteomic Analysis of Histone Variants and Their PTMs: Strategies and Pitfalls}, author = {Sara El Kennani and Marion Crespo and Jérôme Govin and Delphine Pflieger}, doi = {10.3390/proteomes6030029}, issn = {2227-7382}, year = {2018}, date = {2018-01-01}, journal = {Proteomes}, volume = {6}, number = {3}, abstract = {Epigenetic modifications contribute to the determination of cell fate and differentiation. The molecular mechanisms underlying histone variants and post-translational modifications (PTMs) have been studied in the contexts of development, differentiation, and disease. Antibody-based assays have classically been used to target PTMs, but these approaches fail to reveal combinatorial patterns of modifications. In addition, some histone variants are so similar to canonical histones that antibodies have difficulty distinguishing between these isoforms. Mass spectrometry (MS) has progressively developed as a powerful technology for the study of histone variants and their PTMs. Indeed, MS analyses highlighted exquisitely complex combinations of PTMs, suggesting “crosstalk” between them, and also revealed that PTM patterns are often variant-specific. Even though the sensitivity and acquisition speed of MS instruments have considerably increased alongside the development of computational tools for the study of multiple PTMs, it remains challenging to correctly describe the landscape of histone PTMs, and in particular to confidently assign modifications to specific amino acids. Here, we provide an inventory of MS-based strategies and of the pitfalls inherent to histone PTM and variant characterization, while stressing the complex interplay between PTMs and histone sequence variations. We will particularly illustrate the roles played by MS-based analyses in identifying and quantifying histone variants and modifications.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Epigenetic modifications contribute to the determination of cell fate and differentiation. The molecular mechanisms underlying histone variants and post-translational modifications (PTMs) have been studied in the contexts of development, differentiation, and disease. Antibody-based assays have classically been used to target PTMs, but these approaches fail to reveal combinatorial patterns of modifications. In addition, some histone variants are so similar to canonical histones that antibodies have difficulty distinguishing between these isoforms. Mass spectrometry (MS) has progressively developed as a powerful technology for the study of histone variants and their PTMs. Indeed, MS analyses highlighted exquisitely complex combinations of PTMs, suggesting “crosstalk” between them, and also revealed that PTM patterns are often variant-specific. Even though the sensitivity and acquisition speed of MS instruments have considerably increased alongside the development of computational tools for the study of multiple PTMs, it remains challenging to correctly describe the landscape of histone PTMs, and in particular to confidently assign modifications to specific amino acids. Here, we provide an inventory of MS-based strategies and of the pitfalls inherent to histone PTM and variant characterization, while stressing the complex interplay between PTMs and histone sequence variations. We will particularly illustrate the roles played by MS-based analyses in identifying and quantifying histone variants and modifications. |
Kennani, Sara El; Adrait, Annie; Permiakova, Olga; Hesse, Anne-Marie; Ialy-Radio, Côme; Ferro, Myriam; Brun, Virginie; Cocquet, Julie; Govin, Jérôme; Pflieger, Delphine Systematic quantitative analysis of Ħ2A and Ħ2B variants by targeted proteomics Article de journal Epigenetics & Chromatin, 11 (1), p. 2, 2018, ISSN: 1756-8935. @article{el_kennani_systematic_2018, title = {Systematic quantitative analysis of Ħ2A and Ħ2B variants by targeted proteomics}, author = {Sara El Kennani and Annie Adrait and Olga Permiakova and Anne-Marie Hesse and Côme Ialy-Radio and Myriam Ferro and Virginie Brun and Julie Cocquet and Jérôme Govin and Delphine Pflieger}, doi = {10.1186/s13072-017-0172-y}, issn = {1756-8935}, year = {2018}, date = {2018-01-01}, journal = {Epigenetics & Chromatin}, volume = {11}, number = {1}, pages = {2}, abstract = {BACKGROUND: Histones organize DNA into chromatin through a variety of processes. Among them, a vast diversity of histone variants can be incorporated into chromatin and finely modulate its organization and functionality. Classically, the study of histone variants has largely relied on antibody-based assays. However, antibodies have a limited efficiency to discriminate between highly similar histone variants. RESULTS: In this study, we established a mass spectrometry-based analysis to address this challenge. We developed a targeted proteomics method, using selected reaction monitoring or parallel reaction monitoring, to quantify a maximum number of histone variants in a single multiplexed assay, even when histones are present in a crude extract. This strategy was developed on H2A and H2B variants, using 55 peptides corresponding to 25 different histone sequences, among which a few differ by a single amino acid. The methodology was then applied to mouse testis extracts in which almost all histone variants are expressed. It confirmed the abundance profiles of several testis-specific histones during successive stages of spermatogenesis and the existence of predicted H2A.L.1 isoforms. This methodology was also used to explore the over-expression pattern of H2A.L.1 isoforms in a mouse model of male infertility. CONCLUSIONS: Our results demonstrate that targeted proteomics is a powerful method to quantify highly similar histone variants and isoforms. The developed method can be easily transposed to the study of human histone variants, whose abundance can be deregulated in various diseases.}, keywords = {}, pubstate = {published}, tppubtype = {article} } BACKGROUND: Histones organize DNA into chromatin through a variety of processes. Among them, a vast diversity of histone variants can be incorporated into chromatin and finely modulate its organization and functionality. Classically, the study of histone variants has largely relied on antibody-based assays. However, antibodies have a limited efficiency to discriminate between highly similar histone variants. RESULTS: In this study, we established a mass spectrometry-based analysis to address this challenge. We developed a targeted proteomics method, using selected reaction monitoring or parallel reaction monitoring, to quantify a maximum number of histone variants in a single multiplexed assay, even when histones are present in a crude extract. This strategy was developed on H2A and H2B variants, using 55 peptides corresponding to 25 different histone sequences, among which a few differ by a single amino acid. The methodology was then applied to mouse testis extracts in which almost all histone variants are expressed. It confirmed the abundance profiles of several testis-specific histones during successive stages of spermatogenesis and the existence of predicted H2A.L.1 isoforms. This methodology was also used to explore the over-expression pattern of H2A.L.1 isoforms in a mouse model of male infertility. CONCLUSIONS: Our results demonstrate that targeted proteomics is a powerful method to quantify highly similar histone variants and isoforms. The developed method can be easily transposed to the study of human histone variants, whose abundance can be deregulated in various diseases. |
Adam, Céline; Guérois, Raphaël; Citarella, Anna; Verardi, Laura; Adolphe, Florine; Béneut, Claire; Sommermeyer, Vérane; Ramus, Claire; Govin, Jérôme; Couté, Yohann; Borde, Valérie The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes Article de journal PLoS genetics, 14 (2), p. e1007223, 2018, ISSN: 1553-7404. @article{adam_phd_2018, title = {The PHD finger protein Spp1 has distinct functions in the Set1 and the meiotic DSB formation complexes}, author = {Céline Adam and Raphaël Guérois and Anna Citarella and Laura Verardi and Florine Adolphe and Claire Béneut and Vérane Sommermeyer and Claire Ramus and Jérôme Govin and Yohann Couté and Valérie Borde}, doi = {10.1371/journal.pgen.1007223}, issn = {1553-7404}, year = {2018}, date = {2018-01-01}, journal = {PLoS genetics}, volume = {14}, number = {2}, pages = {e1007223}, abstract = {Histone H3K4 methylation is a feature of meiotic recombination hotspots shared by many organisms including plants and mammals. Meiotic recombination is initiated by programmed double-strand break (DSB) formation that in budding yeast takes place in gene promoters and is promoted by histone H3K4 di/trimethylation. This histone modification is recognized by Spp1, a PHD finger containing protein that belongs to the conserved histone H3K4 methyltransferase Set1 complex. During meiosis, Spp1 binds H3K4me3 and interacts with a DSB protein, Mer2, to promote DSB formation close to gene promoters. How Set1 complex- and Mer2- related functions of Spp1 are connected is not clear. Here, combining genome-wide localization analyses, biochemical approaches and the use of separation of function mutants, we show that Spp1 is present within two distinct complexes in meiotic cells, the Set1 and the Mer2 complexes. Disrupting the Spp1-Set1 interaction mildly decreases H3K4me3 levels and does not affect meiotic recombination initiation. Conversely, the Spp1-Mer2 interaction is required for normal meiotic recombination initiation, but dispensable for Set1 complex-mediated histone H3K4 methylation. Finally, we provide evidence that Spp1 preserves normal H3K4me3 levels independently of the Set1 complex. We propose a model where Spp1 works in three ways to promote recombination initiation: first by depositing histone H3K4 methylation (Set1 complex), next by "reading" and protecting histone H3K4 methylation, and finally by making the link with the chromosome axis (Mer2-Spp1 complex). This work deciphers the precise roles of Spp1 in meiotic recombination and opens perspectives to study its functions in other organisms where H3K4me3 is also present at recombination hotspots.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Histone H3K4 methylation is a feature of meiotic recombination hotspots shared by many organisms including plants and mammals. Meiotic recombination is initiated by programmed double-strand break (DSB) formation that in budding yeast takes place in gene promoters and is promoted by histone H3K4 di/trimethylation. This histone modification is recognized by Spp1, a PHD finger containing protein that belongs to the conserved histone H3K4 methyltransferase Set1 complex. During meiosis, Spp1 binds H3K4me3 and interacts with a DSB protein, Mer2, to promote DSB formation close to gene promoters. How Set1 complex- and Mer2- related functions of Spp1 are connected is not clear. Here, combining genome-wide localization analyses, biochemical approaches and the use of separation of function mutants, we show that Spp1 is present within two distinct complexes in meiotic cells, the Set1 and the Mer2 complexes. Disrupting the Spp1-Set1 interaction mildly decreases H3K4me3 levels and does not affect meiotic recombination initiation. Conversely, the Spp1-Mer2 interaction is required for normal meiotic recombination initiation, but dispensable for Set1 complex-mediated histone H3K4 methylation. Finally, we provide evidence that Spp1 preserves normal H3K4me3 levels independently of the Set1 complex. We propose a model where Spp1 works in three ways to promote recombination initiation: first by depositing histone H3K4 methylation (Set1 complex), next by "reading" and protecting histone H3K4 methylation, and finally by making the link with the chromosome axis (Mer2-Spp1 complex). This work deciphers the precise roles of Spp1 in meiotic recombination and opens perspectives to study its functions in other organisms where H3K4me3 is also present at recombination hotspots. |
Muyt, Arnaud De; Pyatnitskaya, Alexandra; Andréani, Jessica; Ranjha, Lepakshi; Ramus, Claire; Laureau, Raphaëlle; Fernandez-Vega, Ambra; Holoch, Daniel; Girard, Elodie; Govin, Jérome; Margueron, Raphaël; Couté, Yohann; Cejka, Petr; Guérois, Raphaël; Borde, Valérie A meiotic XPF-ERCC1-like complex recognizes joint molecule recombination intermediates to promote crossover formation Article de journal Genes & Development, 32 (3-4), p. 283–296, 2018, ISSN: 1549-5477. @article{de_muyt_meiotic_2018, title = {A meiotic XPF-ERCC1-like complex recognizes joint molecule recombination intermediates to promote crossover formation}, author = {Arnaud De Muyt and Alexandra Pyatnitskaya and Jessica Andréani and Lepakshi Ranjha and Claire Ramus and Raphaëlle Laureau and Ambra Fernandez-Vega and Daniel Holoch and Elodie Girard and Jérome Govin and Raphaël Margueron and Yohann Couté and Petr Cejka and Raphaël Guérois and Valérie Borde}, doi = {10.1101/gad.308510.117}, issn = {1549-5477}, year = {2018}, date = {2018-01-01}, journal = {Genes & Development}, volume = {32}, number = {3-4}, pages = {283--296}, abstract = {Meiotic crossover formation requires the stabilization of early recombination intermediates by a set of proteins and occurs within the environment of the chromosome axis, a structure important for the regulation of meiotic recombination events. The molecular mechanisms underlying and connecting crossover recombination and axis localization are elusive. Here, we identified the ZZS (Zip2-Zip4-Spo16) complex, required for crossover formation, which carries two distinct activities: one provided by Zip4, which acts as hub through physical interactions with components of the chromosome axis and the crossover machinery, and the other carried by Zip2 and Spo16, which preferentially bind branched DNA molecules in vitro. We found that Zip2 and Spo16 share structural similarities to the structure-specific XPF-ERCC1 nuclease, although it lacks endonuclease activity. The XPF domain of Zip2 is required for crossover formation, suggesting that, together with Spo16, it has a noncatalytic DNA recognition function. Our results suggest that the ZZS complex shepherds recombination intermediates toward crossovers as a dynamic structural module that connects recombination events to the chromosome axis. The identification of the ZZS complex improves our understanding of the various activities required for crossover implementation and is likely applicable to other organisms, including mammals.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Meiotic crossover formation requires the stabilization of early recombination intermediates by a set of proteins and occurs within the environment of the chromosome axis, a structure important for the regulation of meiotic recombination events. The molecular mechanisms underlying and connecting crossover recombination and axis localization are elusive. Here, we identified the ZZS (Zip2-Zip4-Spo16) complex, required for crossover formation, which carries two distinct activities: one provided by Zip4, which acts as hub through physical interactions with components of the chromosome axis and the crossover machinery, and the other carried by Zip2 and Spo16, which preferentially bind branched DNA molecules in vitro. We found that Zip2 and Spo16 share structural similarities to the structure-specific XPF-ERCC1 nuclease, although it lacks endonuclease activity. The XPF domain of Zip2 is required for crossover formation, suggesting that, together with Spo16, it has a noncatalytic DNA recognition function. Our results suggest that the ZZS complex shepherds recombination intermediates toward crossovers as a dynamic structural module that connects recombination events to the chromosome axis. The identification of the ZZS complex improves our understanding of the various activities required for crossover implementation and is likely applicable to other organisms, including mammals. |
Petosa, Carlo; Govin, Jérôme; Mietton, Flore [A new hope to fight invasive fungal infection] Article de journal Medecine Sciences: M/S, 34 (2), p. 123–125, 2018, ISSN: 1958-5381. @article{petosa_[new_2018, title = {[A new hope to fight invasive fungal infection]}, author = {Carlo Petosa and Jérôme Govin and Flore Mietton}, doi = {10.1051/medsci/20183402007}, issn = {1958-5381}, year = {2018}, date = {2018-01-01}, journal = {Medecine Sciences: M/S}, volume = {34}, number = {2}, pages = {123--125}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Garnaud, Cécile; García-Oliver, Encar; Wang, Yan; Maubon, Danièle; Bailly, Sébastien; Despinasse, Quentin; Champleboux, Morgane; Govin, Jérôme; Cornet, Muriel The Rim Pathway Mediates Antifungal Tolerance in Candida albicans through Newly Identified Rim101 Transcriptional Targets, Including Hsp90 and Ipt1 Article de journal Antimicrobial Agents and Chemotherapy, 62 (3), 2018, ISSN: 1098-6596. @article{garnaud_rim_2018, title = {The Rim Pathway Mediates Antifungal Tolerance in Candida albicans through Newly Identified Rim101 Transcriptional Targets, Including Hsp90 and Ipt1}, author = {Cécile Garnaud and Encar García-Oliver and Yan Wang and Danièle Maubon and Sébastien Bailly and Quentin Despinasse and Morgane Champleboux and Jérôme Govin and Muriel Cornet}, doi = {10.1128/AAC.01785-17}, issn = {1098-6596}, year = {2018}, date = {2018-01-01}, journal = {Antimicrobial Agents and Chemotherapy}, volume = {62}, number = {3}, abstract = {Invasive candidiasis (IC) is a major cause of morbidity and mortality despite antifungal treatment. Azoles and echinocandins are used as first-line therapies for IC. However, their efficacy is limited by yeast tolerance and the emergence of acquired resistance. Tolerance is a reversible stage created due to the yeast's capacity to counter antifungal drug exposure, leading to persistent growth. For Candida albicans, multiple stress signaling pathways have been shown to contribute to this adaptation. Among them, the pH-responsive Rim pathway, through its transcription factor Rim101p, was shown to mediate azole and echinocandin tolerance. The Rim pathway is fungus specific, is conserved among the members of the fungal kingdom, and plays a key role in pathogenesis and virulence. The present study aimed at confirming the role of Rim101p and investigating the implication of the other Rim proteins in antifungal tolerance in C. albicans, as well as the mechanisms underlying it. Time-kill curve experiments and colony formation tests showed that genetic inhibition of all the Rim factors enhances echinocandin and azole antifungal activity. Through RNA sequencing analysis of a rim101-/- mutant, a strain constitutively overexpressing RIM101, and control strains, we discovered novel Rim-dependent genes involved in tolerance, including HSP90, encoding a major molecular chaperone, and IPT1, involved in sphingolipid biosynthesis. Rim mutants were also hypersensitive to pharmacological inhibition of Hsp90. Taken together, these data suggest that Rim101 acts upstream of Hsp90 and that targeting the Rim pathway in combination with existing antifungal drugs may represent a promising antifungal strategy to indirectly but specifically target Hsp90 in yeasts.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Invasive candidiasis (IC) is a major cause of morbidity and mortality despite antifungal treatment. Azoles and echinocandins are used as first-line therapies for IC. However, their efficacy is limited by yeast tolerance and the emergence of acquired resistance. Tolerance is a reversible stage created due to the yeast's capacity to counter antifungal drug exposure, leading to persistent growth. For Candida albicans, multiple stress signaling pathways have been shown to contribute to this adaptation. Among them, the pH-responsive Rim pathway, through its transcription factor Rim101p, was shown to mediate azole and echinocandin tolerance. The Rim pathway is fungus specific, is conserved among the members of the fungal kingdom, and plays a key role in pathogenesis and virulence. The present study aimed at confirming the role of Rim101p and investigating the implication of the other Rim proteins in antifungal tolerance in C. albicans, as well as the mechanisms underlying it. Time-kill curve experiments and colony formation tests showed that genetic inhibition of all the Rim factors enhances echinocandin and azole antifungal activity. Through RNA sequencing analysis of a rim101-/- mutant, a strain constitutively overexpressing RIM101, and control strains, we discovered novel Rim-dependent genes involved in tolerance, including HSP90, encoding a major molecular chaperone, and IPT1, involved in sphingolipid biosynthesis. Rim mutants were also hypersensitive to pharmacological inhibition of Hsp90. Taken together, these data suggest that Rim101 acts upstream of Hsp90 and that targeting the Rim pathway in combination with existing antifungal drugs may represent a promising antifungal strategy to indirectly but specifically target Hsp90 in yeasts. |
He, Huan; Brenier-Pinchart, Marie-Pierre; Braun, Laurence; Kraut, Alexandra; Touquet, Bastien; Couté, Yohann; Tardieux, Isabelle; Hakimi, Mohamed-Ali; Bougdour, Alexandre Characterization of a Toxoplasma effector uncovers an alternative GSK3/β-catenin-regulatory pathway of inflammation Article de journal eLife, 7 , 2018, ISSN: 2050-084X. @article{he_characterization_2018, title = {Characterization of a Toxoplasma effector uncovers an alternative GSK3/β-catenin-regulatory pathway of inflammation}, author = {Huan He and Marie-Pierre Brenier-Pinchart and Laurence Braun and Alexandra Kraut and Bastien Touquet and Yohann Couté and Isabelle Tardieux and Mohamed-Ali Hakimi and Alexandre Bougdour}, doi = {10.7554/eLife.39887}, issn = {2050-084X}, year = {2018}, date = {2018-01-01}, journal = {eLife}, volume = {7}, abstract = {The intracellular parasite Toxoplasma gondii, hijacks evolutionarily conserved host processes by delivering effector proteins into the host cell that shift gene expression in a timely fashion. We identified a parasite dense granule protein as GRA18 that once released in the host cell cytoplasm forms versatile complexes with regulatory elements of the β-catenin destruction complex. By interacting with GSK3/PP2A-B56, GRA18 drives β-catenin up-regulation and the downstream effects on host cell gene expression. In the context of macrophages infection, GRA18 induces the expression of a specific set of genes commonly associated with an anti-inflammatory response that includes those encoding chemokines CCL17 and CCL22. Overall, this study adds another original strategy by which T. gondii tachyzoites reshuffle the host cell interactome through a GSK3/β-catenin axis to selectively reprogram immune gene expression.}, keywords = {}, pubstate = {published}, tppubtype = {article} } The intracellular parasite Toxoplasma gondii, hijacks evolutionarily conserved host processes by delivering effector proteins into the host cell that shift gene expression in a timely fashion. We identified a parasite dense granule protein as GRA18 that once released in the host cell cytoplasm forms versatile complexes with regulatory elements of the β-catenin destruction complex. By interacting with GSK3/PP2A-B56, GRA18 drives β-catenin up-regulation and the downstream effects on host cell gene expression. In the context of macrophages infection, GRA18 induces the expression of a specific set of genes commonly associated with an anti-inflammatory response that includes those encoding chemokines CCL17 and CCL22. Overall, this study adds another original strategy by which T. gondii tachyzoites reshuffle the host cell interactome through a GSK3/β-catenin axis to selectively reprogram immune gene expression. |
Picard, Marion A L; Cosseau, Celine; Ferré, Sabrina; Quack, Thomas; Grevelding, Christoph G; Couté, Yohann; Vicoso, Beatriz Evolution of gene dosage on the Z-chromosome of schistosome parasites Article de journal eLife, 7 , 2018, ISSN: 2050-084X. @article{picard_evolution_2018, title = {Evolution of gene dosage on the Z-chromosome of schistosome parasites}, author = {Marion A L Picard and Celine Cosseau and Sabrina Ferré and Thomas Quack and Christoph G Grevelding and Yohann Couté and Beatriz Vicoso}, doi = {10.7554/eLife.35684}, issn = {2050-084X}, year = {2018}, date = {2018-01-01}, journal = {eLife}, volume = {7}, abstract = {XY systems usually show chromosome-wide compensation of X-linked genes, while in many ZW systems, compensation is restricted to a minority of dosage-sensitive genes. Why such differences arose is still unclear. Here, we combine comparative genomics, transcriptomics and proteomics to obtain a complete overview of the evolution of gene dosage on the Z-chromosome of Schistosoma parasites. We compare the Z-chromosome gene content of African (Schistosoma mansoni and S. haematobium) and Asian (S. japonicum) schistosomes and describe lineage-specific evolutionary strata. We use these to assess gene expression evolution following sex-linkage. The resulting patterns suggest a reduction in expression of Z-linked genes in females, combined with upregulation of the Z in both sexes, in line with the first step of Ohno's classic model of dosage compensation evolution. Quantitative proteomics suggest that post-transcriptional mechanisms do not play a major role in balancing the expression of Z-linked genes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } XY systems usually show chromosome-wide compensation of X-linked genes, while in many ZW systems, compensation is restricted to a minority of dosage-sensitive genes. Why such differences arose is still unclear. Here, we combine comparative genomics, transcriptomics and proteomics to obtain a complete overview of the evolution of gene dosage on the Z-chromosome of Schistosoma parasites. We compare the Z-chromosome gene content of African (Schistosoma mansoni and S. haematobium) and Asian (S. japonicum) schistosomes and describe lineage-specific evolutionary strata. We use these to assess gene expression evolution following sex-linkage. The resulting patterns suggest a reduction in expression of Z-linked genes in females, combined with upregulation of the Z in both sexes, in line with the first step of Ohno's classic model of dosage compensation evolution. Quantitative proteomics suggest that post-transcriptional mechanisms do not play a major role in balancing the expression of Z-linked genes. |
Legendre, Matthieu; Fabre, Elisabeth; Poirot, Olivier; Jeudy, Sandra; Lartigue, Audrey; Alempic, Jean-Marie; Beucher, Laure; Philippe, Nadège; Bertaux, Lionel; Christo-Foroux, Eugène; Labadie, Karine; Couté, Yohann; Abergel, Chantal; Claverie, Jean-Michel Diversity and evolution of the emerging Pandoraviridae family Article de journal Nature Communications, 9 (1), p. 2285, 2018, ISSN: 2041-1723. @article{legendre_diversity_2018, title = {Diversity and evolution of the emerging Pandoraviridae family}, author = {Matthieu Legendre and Elisabeth Fabre and Olivier Poirot and Sandra Jeudy and Audrey Lartigue and Jean-Marie Alempic and Laure Beucher and Nadège Philippe and Lionel Bertaux and Eugène Christo-Foroux and Karine Labadie and Yohann Couté and Chantal Abergel and Jean-Michel Claverie}, doi = {10.1038/s41467-018-04698-4}, issn = {2041-1723}, year = {2018}, date = {2018-01-01}, journal = {Nature Communications}, volume = {9}, number = {1}, pages = {2285}, abstract = {With DNA genomes reaching 2.5 Mb packed in particles of bacterium-like shape and dimension, the first two Acanthamoeba-infecting pandoraviruses remained up to now the most complex viruses since their discovery in 2013. Our isolation of three new strains from distant locations and environments is now used to perform the first comparative genomics analysis of the emerging worldwide-distributed Pandoraviridae family. Thorough annotation of the genomes combining transcriptomic, proteomic, and bioinformatic analyses reveals many non-coding transcripts and significantly reduces the former set of predicted protein-coding genes. Here we show that the pandoraviruses exhibit an open pan-genome, the enormous size of which is not adequately explained by gene duplications or horizontal transfers. As most of the strain-specific genes have no extant homolog and exhibit statistical features comparable to intergenic regions, we suggest that de novo gene creation could contribute to the evolution of the giant pandoravirus genomes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } With DNA genomes reaching 2.5 Mb packed in particles of bacterium-like shape and dimension, the first two Acanthamoeba-infecting pandoraviruses remained up to now the most complex viruses since their discovery in 2013. Our isolation of three new strains from distant locations and environments is now used to perform the first comparative genomics analysis of the emerging worldwide-distributed Pandoraviridae family. Thorough annotation of the genomes combining transcriptomic, proteomic, and bioinformatic analyses reveals many non-coding transcripts and significantly reduces the former set of predicted protein-coding genes. Here we show that the pandoraviruses exhibit an open pan-genome, the enormous size of which is not adequately explained by gene duplications or horizontal transfers. As most of the strain-specific genes have no extant homolog and exhibit statistical features comparable to intergenic regions, we suggest that de novo gene creation could contribute to the evolution of the giant pandoravirus genomes. |
Eymard-Vernain, Elise; Couté, Yohann; Adrait, Annie; Rabilloud, Thierry; Sarret, Géraldine; Lelong, Cécile The poly-gamma-glutamate of Bacillus subtilis interacts specifically with silver nanoparticles Article de journal PloS One, 13 (5), p. e0197501, 2018, ISSN: 1932-6203. @article{eymard-vernain_poly-gamma-glutamate_2018, title = {The poly-gamma-glutamate of Bacillus subtilis interacts specifically with silver nanoparticles}, author = {Elise Eymard-Vernain and Yohann Couté and Annie Adrait and Thierry Rabilloud and Géraldine Sarret and Cécile Lelong}, doi = {10.1371/journal.pone.0197501}, issn = {1932-6203}, year = {2018}, date = {2018-01-01}, journal = {PloS One}, volume = {13}, number = {5}, pages = {e0197501}, abstract = {For many years, silver nanoparticles, as with other antibacterial nanoparticles, have been extensively used in manufactured products. However, their fate in the environment is unclear and raises questions. We studied the fate of silver nanoparticles in the presence of bacteria under growth conditions that are similar to those found naturally in the environment (that is, bacteria in a stationary phase with low nutrient concentrations). We demonstrated that the viability and the metabolism of a gram-positive bacteria, Bacillus subtilis, exposed during the stationary phase is unaffected by 1 mg/L of silver nanoparticles. These results can be partly explained by a physical interaction of the poly-gamma-glutamate (PGA) secreted by Bacillus subtilis with the silver nanoparticles. The coating of the silver nanoparticles by the secreted PGA likely results in a loss of the bioavailability of nanoparticles and, consequently, a decrease of their biocidal effect.}, keywords = {}, pubstate = {published}, tppubtype = {article} } For many years, silver nanoparticles, as with other antibacterial nanoparticles, have been extensively used in manufactured products. However, their fate in the environment is unclear and raises questions. We studied the fate of silver nanoparticles in the presence of bacteria under growth conditions that are similar to those found naturally in the environment (that is, bacteria in a stationary phase with low nutrient concentrations). We demonstrated that the viability and the metabolism of a gram-positive bacteria, Bacillus subtilis, exposed during the stationary phase is unaffected by 1 mg/L of silver nanoparticles. These results can be partly explained by a physical interaction of the poly-gamma-glutamate (PGA) secreted by Bacillus subtilis with the silver nanoparticles. The coating of the silver nanoparticles by the secreted PGA likely results in a loss of the bioavailability of nanoparticles and, consequently, a decrease of their biocidal effect. |
Abulfaraj, Aala A; Mariappan, Kiruthiga; Bigeard, Jean; Manickam, Prabhu; Blilou, Ikram; Guo, Xiujie; Al-Babili, Salim; Pflieger, Delphine; Hirt, Heribert; Rayapuram, Naganand The Arabidopsis homolog of human G3BP1 is a key regulator of stomatal and apoplastic immunity Article de journal Life Science Alliance, 1 (2), p. e201800046, 2018, ISSN: 2575-1077. @article{abulfaraj_arabidopsis_2018, title = {The Arabidopsis homolog of human G3BP1 is a key regulator of stomatal and apoplastic immunity}, author = {Aala A Abulfaraj and Kiruthiga Mariappan and Jean Bigeard and Prabhu Manickam and Ikram Blilou and Xiujie Guo and Salim Al-Babili and Delphine Pflieger and Heribert Hirt and Naganand Rayapuram}, doi = {10.26508/lsa.201800046}, issn = {2575-1077}, year = {2018}, date = {2018-01-01}, journal = {Life Science Alliance}, volume = {1}, number = {2}, pages = {e201800046}, abstract = {Mammalian Ras-GTPase-activating protein SH3-domain-binding proteins (G3BPs) are a highly conserved family of RNA-binding proteins that link kinase receptor-mediated signaling to RNA metabolism. Mammalian G3BP1 is a multifunctional protein that functions in viral immunity. Here, we show that the Arabidopsis thaliana homolog of human G3BP1 negatively regulates plant immunity. Arabidopsis g3bp1 mutants showed enhanced resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato. Pathogen resistance was mediated in Atg3bp1 mutants by altered stomatal and apoplastic immunity. Atg3bp1 mutants restricted pathogen entry into stomates showing insensitivity to bacterial coronatine-mediated stomatal reopening. AtG3BP1 was identified as a negative regulator of defense responses, which correlated with moderate up-regulation of salicylic acid biosynthesis and signaling without growth penalty.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Mammalian Ras-GTPase-activating protein SH3-domain-binding proteins (G3BPs) are a highly conserved family of RNA-binding proteins that link kinase receptor-mediated signaling to RNA metabolism. Mammalian G3BP1 is a multifunctional protein that functions in viral immunity. Here, we show that the Arabidopsis thaliana homolog of human G3BP1 negatively regulates plant immunity. Arabidopsis g3bp1 mutants showed enhanced resistance to the virulent bacterial pathogen Pseudomonas syringae pv. tomato. Pathogen resistance was mediated in Atg3bp1 mutants by altered stomatal and apoplastic immunity. Atg3bp1 mutants restricted pathogen entry into stomates showing insensitivity to bacterial coronatine-mediated stomatal reopening. AtG3BP1 was identified as a negative regulator of defense responses, which correlated with moderate up-regulation of salicylic acid biosynthesis and signaling without growth penalty. |
Sage, Eric; Sansa, Marc; Fostner, Shawn; Defoort, Martial; Gély, Marc; Naik, Akshay K; Morel, Robert; Duraffourg, Laurent; Roukes, Michael L; Alava, Thomas; Jourdan, Guillaume; Colinet, Eric; Masselon, Christophe; Brenac, Ariel; Hentz, Sébastien Single-particle mass spectrometry with arrays of frequency-addressed nanomechanical resonators Article de journal Nature Communications, 9 (1), p. 3283, 2018, ISSN: 2041-1723. @article{sage_single-particle_2018, title = {Single-particle mass spectrometry with arrays of frequency-addressed nanomechanical resonators}, author = {Eric Sage and Marc Sansa and Shawn Fostner and Martial Defoort and Marc Gély and Akshay K Naik and Robert Morel and Laurent Duraffourg and Michael L Roukes and Thomas Alava and Guillaume Jourdan and Eric Colinet and Christophe Masselon and Ariel Brenac and Sébastien Hentz}, doi = {10.1038/s41467-018-05783-4}, issn = {2041-1723}, year = {2018}, date = {2018-01-01}, journal = {Nature Communications}, volume = {9}, number = {1}, pages = {3283}, abstract = {One of the main challenges to overcome to perform nanomechanical mass spectrometry analysis in a practical time frame stems from the size mismatch between the analyte beam and the small nanomechanical detector area. We report here the demonstration of mass spectrometry with arrays of 20 multiplexed nanomechanical resonators; each resonator is designed with a distinct resonance frequency which becomes its individual address. Mass spectra of metallic aggregates in the MDa range are acquired with more than one order of magnitude improvement in analysis time compared to individual resonators. A 20 NEMS array is probed in 150 ms with the same mass limit of detection as a single resonator. Spectra acquired with a conventional time-of-flight mass spectrometer in the same system show excellent agreement. We also demonstrate how mass spectrometry imaging at the single-particle level becomes possible by mapping a 4-cm-particle beam in the MDa range and above.}, keywords = {}, pubstate = {published}, tppubtype = {article} } One of the main challenges to overcome to perform nanomechanical mass spectrometry analysis in a practical time frame stems from the size mismatch between the analyte beam and the small nanomechanical detector area. We report here the demonstration of mass spectrometry with arrays of 20 multiplexed nanomechanical resonators; each resonator is designed with a distinct resonance frequency which becomes its individual address. Mass spectra of metallic aggregates in the MDa range are acquired with more than one order of magnitude improvement in analysis time compared to individual resonators. A 20 NEMS array is probed in 150 ms with the same mass limit of detection as a single resonator. Spectra acquired with a conventional time-of-flight mass spectrometer in the same system show excellent agreement. We also demonstrate how mass spectrometry imaging at the single-particle level becomes possible by mapping a 4-cm-particle beam in the MDa range and above. |
Berthier, A; Vinod, M; Porez, G; Steenackers, A; Alexandre, J; Yamakawa, N; Gheeraert, C; Ploton, M; Maréchal, X; Dubois-Chevalier, J; Hovasse, A; Schaeffer-Reiss, C; Cianférani, S; Rolando, C; Bray, F; Duez, H; Eeckhoute, J; Lefebvre, T; Staels, B; Lefebvre, P Combinatorial regulation of hepatic cytoplasmic signaling and nuclear transcriptional events by the OGT/REV-ERBα complex. Article de journal Proc Natl Acad Sci U S A., 15 (47), p. E11033-E11042., 2018. @article{621, title = {Combinatorial regulation of hepatic cytoplasmic signaling and nuclear transcriptional events by the OGT/REV-ERBα complex.}, author = {A Berthier and M Vinod and G Porez and A Steenackers and J Alexandre and N Yamakawa and C Gheeraert and M Ploton and X Maréchal and J Dubois-Chevalier and A Hovasse and C Schaeffer-Reiss and S Cianférani and C Rolando and F Bray and H Duez and J Eeckhoute and T Lefebvre and B Staels and P Lefebvre}, doi = {10.1073/pnas.1805397115}, year = {2018}, date = {2018-01-01}, journal = {Proc Natl Acad Sci U S A.}, volume = {15}, number = {47}, pages = {E11033-E11042.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Bibi-Triki, S; Husson, G; Maucourt, B; Vuilleumier, S; Carapito, C; Bringel, F N-terminome and proteogenomic analysis of the Methylobacterium extorquens DM4 reference strain for dichloromethane utilization. Article de journal J Proteomics., (179), p. 131-139, 2018. @article{606, title = {N-terminome and proteogenomic analysis of the Methylobacterium extorquens DM4 reference strain for dichloromethane utilization.}, author = {S Bibi-Triki and G Husson and B Maucourt and S Vuilleumier and C Carapito and F Bringel}, year = {2018}, date = {2018-01-01}, journal = {J Proteomics.}, number = {179}, pages = {131-139}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Boeuf, A; Schnell, G; Bernard, Q; Kern, A; Westermann, B; Ehret-Sabatier, L; Grillon, A; Schramm, F; Jaulhac, B; Boulanger, N Dissociating effect of salivary gland extract from Ixodes ricinus on human fibroblasts: Potential impact on Borrelia transmission. Article de journal Ticks Tick Borne Dis., pii: S1877-959X(18)30014-1. , 2018, (2012/29). @article{626, title = {Dissociating effect of salivary gland extract from Ixodes ricinus on human fibroblasts: Potential impact on Borrelia transmission.}, author = {A Boeuf and G Schnell and Q Bernard and A Kern and B Westermann and L Ehret-Sabatier and A Grillon and F Schramm and B Jaulhac and N Boulanger}, year = {2018}, date = {2018-01-01}, journal = {Ticks Tick Borne Dis.}, volume = {pii: S1877-959X(18)30014-1.}, note = {2012/29}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Carapito, R; Carapito, C; Morlon, A; Paul, N; Jacome, AS. Vaca; Alsaleh, G; Rolli, V; Tahar, O; Aouadi, I; Rompais, M; Delalande, F; Pichot, A; Georgel, P; Messer, L; Sibilia, J; Cianferani, S; Dorsselaer, Van A; Bahram, S Multi-OMICS analyses unveil STAT1 as a potential modifier gene in mevalonate kinase deficiency. Article de journal Ann Rheum Dis., 77 (11), p. 1675-1687., 2018. @article{616, title = {Multi-OMICS analyses unveil STAT1 as a potential modifier gene in mevalonate kinase deficiency.}, author = {R Carapito and C Carapito and A Morlon and N Paul and AS. Vaca Jacome and G Alsaleh and V Rolli and O Tahar and I Aouadi and M Rompais and F Delalande and A Pichot and P Georgel and L Messer and J Sibilia and S Cianferani and A Van Dorsselaer and S Bahram}, year = {2018}, date = {2018-01-01}, journal = {Ann Rheum Dis.}, volume = {77}, number = {11}, pages = {1675-1687.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Cavallo, G; Poyer, S; Amalian, JA.; Dufour, F; Burel, A; Carapito, C; Charles, L; Lutz, JF. Cleavable Binary Dyads: Simplifying Data Extraction and Increasing Storage Density in Digital Polymers. Article de journal Angew Chem Int Ed Engl., 57 (21), p. 6266-6269., 2018. @article{605, title = {Cleavable Binary Dyads: Simplifying Data Extraction and Increasing Storage Density in Digital Polymers.}, author = {G Cavallo and S Poyer and JA. Amalian and F Dufour and A Burel and C Carapito and L Charles and JF. Lutz}, year = {2018}, date = {2018-01-01}, journal = {Angew Chem Int Ed Engl.}, volume = {57}, number = {21}, pages = {6266-6269.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chanon, S; Chazarin, B; Toubhans, B; Durand, C; Chery, I; Robert, M; Vieille-Marchiset, A; Swenson, J E; Zedrosser, A; Evans, A L; Brunberg, S; Arnemo, J M; Gauquelin-Koch, G; Storey, K B; Simon, C; Blanc, S; Bertile, F; Lefai, E Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells Article de journal Sci Rep 2018, 8 (1), p. 5525, 2018, (2011/34). @article{597, title = {Proteolysis inhibition by hibernating bear serum leads to increased protein content in human muscle cells}, author = {S Chanon and B Chazarin and B Toubhans and C Durand and I Chery and M Robert and A Vieille-Marchiset and J E. Swenson and A Zedrosser and A L. Evans and S Brunberg and J M. Arnemo and G Gauquelin-Koch and K B. Storey and C Simon and S Blanc and F Bertile and E Lefai}, doi = {10.1038/s41598-018-23891-5}, year = {2018}, date = {2018-01-01}, journal = {Sci Rep 2018}, volume = {8}, number = {1}, pages = {5525}, note = {2011/34}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chopard, C; Tong, PBV.; Tóth, P; Schatz, M; Yezid, H; Debaisieux, S; Mettling, C; Gross, A; Pugnière, M; Tu, A; Strub, JM.; Mesnard, JM.; Vitale, N; Beaumelle, B Cyclophilin A enables specific HIV-1 Tat palmitoylation and accumulation in uninfected cells. Article de journal Nat Commun., 2018, (2006/01). @article{608, title = {Cyclophilin A enables specific HIV-1 Tat palmitoylation and accumulation in uninfected cells.}, author = {C Chopard and PBV. Tong and P Tóth and M Schatz and H Yezid and S Debaisieux and C Mettling and A Gross and M Pugnière and A Tu and JM. Strub and JM. Mesnard and N Vitale and B Beaumelle}, year = {2018}, date = {2018-01-01}, journal = {Nat Commun.}, note = {2006/01}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Coulter, ME.; Dorobantu, CM.; Lodewijk, GA.; Delalande, F; Cianferani, S; Ganesh, VS.; Smith, RS.; Lim, ET.; Xu, CS.; Pang, S; Wong, ET.; Lidov, HGW.; Calicchio, ML.; Yang, E; Gonzalez, DM.; Schlaeger, TM.; Mochida, GH.; Hess, H; Lee, WA.; Lehtinen, MK.; Kirchhausen, T; Haussler, D; Jacobs, FMJ.; Gaudin, R; Walsh, CA. The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles. Article de journal Cell Rep., 24 (4), p. 973-986.e8., 2018. @article{614, title = {The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.}, author = {ME. Coulter and CM. Dorobantu and GA. Lodewijk and F Delalande and S Cianferani and VS. Ganesh and RS. Smith and ET. Lim and CS. Xu and S Pang and ET. Wong and HGW. Lidov and ML. Calicchio and E Yang and DM. Gonzalez and TM. Schlaeger and GH. Mochida and H Hess and WA. Lee and MK. Lehtinen and T Kirchhausen and D Haussler and FMJ. Jacobs and R Gaudin and CA. Walsh}, year = {2018}, date = {2018-01-01}, journal = {Cell Rep.}, volume = {24}, number = {4}, pages = {973-986.e8.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Criscuolo, F; Sorci, G; Behaim-Delarbre, M; Zahn, S; Faivre, B; Bertile, F Age-related response to an acute innate immune challenge in mice: proteomics reveals a telomere maintenance-related cost. Article de journal Proceedings of the Royal Society B, 285 , p. 20181877., 2018, (2012/28). @article{622, title = {Age-related response to an acute innate immune challenge in mice: proteomics reveals a telomere maintenance-related cost.}, author = {F Criscuolo and G Sorci and M Behaim-Delarbre and S Zahn and B Faivre and F Bertile}, year = {2018}, date = {2018-01-01}, journal = {Proceedings of the Royal Society B}, volume = {285}, pages = {20181877.}, note = {2012/28}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dartevelle, P; Ehlinger, C; Zaet, A; Boehler, C; Rabineau, M; Westermann, B; Strub, JM.; Cianferani, S; Haïkel, Y; Metz-Boutigue, MH.; Marban, C D-Cateslytin: a new antifungal agent for the treatment of oral Candida albicans associated infections. Article de journal Sci Rep., 8 (1), p. 9235., 2018, (2009/25). @article{600, title = {D-Cateslytin: a new antifungal agent for the treatment of oral Candida albicans associated infections.}, author = {P Dartevelle and C Ehlinger and A Zaet and C Boehler and M Rabineau and B Westermann and JM. Strub and S Cianferani and Y Haïkel and MH. Metz-Boutigue and C Marban}, year = {2018}, date = {2018-01-01}, journal = {Sci Rep.}, volume = {8}, number = {1}, pages = {9235.}, note = {2009/25}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
D'Atri, V; Causon, T; Hernandez-Alba, O; Mutabazi, A; Veuthey, JL.; Cianferani, S; Guillarme, D Adding a new separation dimension to MS and LC-MS: What is the utility of ion mobility spectrometry? Article de journal J Sep Sci., 41 (1), p. 20-67., 2018, (0 (review)). @article{582, title = {Adding a new separation dimension to MS and LC-MS: What is the utility of ion mobility spectrometry?}, author = {V D'Atri and T Causon and O Hernandez-Alba and A Mutabazi and JL. Veuthey and S Cianferani and D Guillarme}, year = {2018}, date = {2018-01-01}, journal = {J Sep Sci.}, volume = {41}, number = {1}, pages = {20-67.}, note = {0 (review)}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Desplancq, D; Groysbeck, N; Chiper, M; Weiss, E; Frisch, B; Strub, JM.; Cianférani, S; Zafeiratos, S; Moeglin, E; Holy, X; a.L. Favier,; Decarlo, S; Schultz, P; Spehner, D; Zuber, G Cytosolic Diffusion and Peptide-assisted Nuclear Shuttling of Peptide-substituted circa 102 Gold Atom Nanoclusters in Living Cells Article de journal ACS Applied Nano Materials, 1 , p. 4236-4246, 2018, (2018/02). @article{619, title = {Cytosolic Diffusion and Peptide-assisted Nuclear Shuttling of Peptide-substituted circa 102 Gold Atom Nanoclusters in Living Cells}, author = {D Desplancq and N Groysbeck and M Chiper and E Weiss and B Frisch and JM. Strub and S Cianférani and S Zafeiratos and E Moeglin and X Holy and a.L. Favier and S Decarlo and P Schultz and D Spehner and G Zuber}, year = {2018}, date = {2018-01-01}, journal = {ACS Applied Nano Materials}, volume = {1}, pages = {4236-4246}, note = {2018/02}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dovgan, I; Erb, S; Hessmann, S; Ursuegui, S; Michel, C; Muller, C; Chaubet, G; Cianférani, S; Wagner, A Arginine-selective bioconjugation with 4-azidophenyl glyoxal: application to the single and dual functionalisation of native antibodies. Article de journal Org Biomol Chem., in press (Feb 1. doi: 10.1039/c7ob02844j.), 2018, (2017/21). @article{595, title = {Arginine-selective bioconjugation with 4-azidophenyl glyoxal: application to the single and dual functionalisation of native antibodies.}, author = {I Dovgan and S Erb and S Hessmann and S Ursuegui and C Michel and C Muller and G Chaubet and S Cianférani and A Wagner}, year = {2018}, date = {2018-01-01}, journal = {Org Biomol Chem.}, volume = {in press}, number = {Feb 1. doi: 10.1039/c7ob02844j.}, note = {2017/21}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ehkirch, A; D'Atri, V; Rouvière, F; Hernandez-Alba, O; Goyon, A; Colas, O; Sarrut, M; Beck, A; Guillarme, D; Heinisch, S; Cianférani, S An online four-dimensional HICxSEC-IMxMS methodology for proof-of-concept characterization of antibody drug conjugates. Article de journal Anal Chem., 90 (3), p. 1578-1586., 2018. @article{591, title = {An online four-dimensional HICxSEC-IMxMS methodology for proof-of-concept characterization of antibody drug conjugates.}, author = {A Ehkirch and V D'Atri and F Rouvière and O Hernandez-Alba and A Goyon and O Colas and M Sarrut and A Beck and D Guillarme and S Heinisch and S Cianférani}, year = {2018}, date = {2018-01-01}, journal = {Anal Chem.}, volume = {90}, number = {3}, pages = {1578-1586.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ehkirch, A; Goyon, A; Hernandez-Alba, O; Rouviere, F; D'Atri, V; Dreyfus, C; Haeuw, JF.; Diemer, H; Beck, A; Heinisch, S; Guillarme, D; Cianferani, S A Novel Online Four-Dimensional SEC×SEC-IM×MS Methodology for Characterization of Monoclonal Antibody Size Variants. Article de journal Anal Chem., Anal Chem. 2018 Nov 12. , p. doi: 10.1021/acs.analchem.8b03333., 2018, (2017/12). @article{620, title = {A Novel Online Four-Dimensional SEC×SEC-IM×MS Methodology for Characterization of Monoclonal Antibody Size Variants.}, author = {A Ehkirch and A Goyon and O Hernandez-Alba and F Rouviere and V D'Atri and C Dreyfus and JF. Haeuw and H Diemer and A Beck and S Heinisch and D Guillarme and S Cianferani}, year = {2018}, date = {2018-01-01}, journal = {Anal Chem.}, volume = {Anal Chem. 2018 Nov 12.}, pages = {doi: 10.1021/acs.analchem.8b03333.}, note = {2017/12}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ehkirch, A; Hernandez-Alba, O; Colas, O; Beck, A; Guillarme, D; Cianférani, S Hyphenation of size exclusion chromatography to native ion mobility mass spectrometry for the analytical characterization of therapeutic antibodies and related products. Article de journal J Chromatogr B Analyt Technol Biomed Life Sci., (1086), p. 176-183., 2018, (2016/14). @article{604, title = {Hyphenation of size exclusion chromatography to native ion mobility mass spectrometry for the analytical characterization of therapeutic antibodies and related products.}, author = {A Ehkirch and O Hernandez-Alba and O Colas and A Beck and D Guillarme and S Cianférani}, year = {2018}, date = {2018-01-01}, journal = {J Chromatogr B Analyt Technol Biomed Life Sci.}, number = {1086}, pages = {176-183.}, note = {2016/14}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ehrmann, FR.; Kalim, J; Pfaffeneder, T; Bernet, B; Hohn, C; Schäfer, E; Botzanowski, T; Cianférani, S; Heine, A; Reuter, K; Diederich, F; Klebe, G Swapping Interface Contacts in the Homodimeric tRNA Guanine Transglycosylase: An Option for Functional Regulation. Article de journal Angew Chem Int Ed Engl., 57 (32), p. 10085-10090., 2018, (2007/53). @article{599, title = {Swapping Interface Contacts in the Homodimeric tRNA Guanine Transglycosylase: An Option for Functional Regulation.}, author = {FR. Ehrmann and J Kalim and T Pfaffeneder and B Bernet and C Hohn and E Schäfer and T Botzanowski and S Cianférani and A Heine and K Reuter and F Diederich and G Klebe}, year = {2018}, date = {2018-01-01}, journal = {Angew Chem Int Ed Engl.}, volume = {57}, number = {32}, pages = {10085-10090.}, note = {2007/53}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Garcia, L; Girod, M; Rompais, M; Dugourd, P; Carapito, C; Lemoine, J Data-Independent Acquisition Coupled to Visible Laser-Induced Dissociation at 473 nm (DIA-LID) for Peptide-Centric Specific Analysis of Cysteine-Containing Peptide Subset. Article de journal Anal Chem., 90 (6), p. 3928-3935., 2018. @article{607, title = {Data-Independent Acquisition Coupled to Visible Laser-Induced Dissociation at 473 nm (DIA-LID) for Peptide-Centric Specific Analysis of Cysteine-Containing Peptide Subset.}, author = {L Garcia and M Girod and M Rompais and P Dugourd and C Carapito and J Lemoine}, year = {2018}, date = {2018-01-01}, journal = {Anal Chem.}, volume = {90}, number = {6}, pages = {3928-3935.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Giroud, S; Evans, AL.; Chery, I; Bertile, F; Tascher, G; Bertrand-Michel, J; Gauquelin-Koch, G; Arnemo, JM.; Swenson, JE.; Lefai, E; Blanc, S; Simon, C Seasonal changes in eicosanoid metabolism in the brown bear. Article de journal Naturwissenschaften, 105 (9-10), p. 58, 2018, (2011/34). @article{610, title = {Seasonal changes in eicosanoid metabolism in the brown bear.}, author = {S Giroud and AL. Evans and I Chery and F Bertile and G Tascher and J Bertrand-Michel and G Gauquelin-Koch and JM. Arnemo and JE. Swenson and E Lefai and S Blanc and C Simon}, year = {2018}, date = {2018-01-01}, journal = {Naturwissenschaften}, volume = {105}, number = {9-10}, pages = {58}, note = {2011/34}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Grzela, R; Nusbaum, J; Fieulaine, S; Lavecchia, F; Desmadril, M; Nhiri, N; Dorsselaer, Van A; Cianferani, S; Jacquet, E; Meinnel, T; Giglione, C Peptide deformylases from Vibrio parahaemolyticus phage and bacteria display similar deformylase activity and inhibitor binding clefts. Article de journal Biochim Biophys Acta., 1866 (2), p. 348-355., 2018. @article{592, title = {Peptide deformylases from Vibrio parahaemolyticus phage and bacteria display similar deformylase activity and inhibitor binding clefts.}, author = {R Grzela and J Nusbaum and S Fieulaine and F Lavecchia and M Desmadril and N Nhiri and A Van Dorsselaer and S Cianferani and E Jacquet and T Meinnel and C Giglione}, year = {2018}, date = {2018-01-01}, journal = {Biochim Biophys Acta.}, volume = {1866}, number = {2}, pages = {348-355.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Henri, J; Chagot, ME.; Bourguet, M; Abel, Y; Terral, G; Maurizy, C; Aigueperse, C; Georgescauld, F; Vandermoere, F; Saint-Fort, R; Behm-Ansmant, I; Charpentier, B; Pradet-Balade, B; Verheggen, C; Bertrand, E; Meyer, P; Cianférani, S; Manival, X; Quinternet, M Deep Structural Analysis of RPAP3 and PIH1D1, Two Components of the HSP90 Co-chaperone R2TP Complex. Article de journal Structure, 26 (9), p. 1196-1209.e8., 2018. @article{615, title = {Deep Structural Analysis of RPAP3 and PIH1D1, Two Components of the HSP90 Co-chaperone R2TP Complex.}, author = {J Henri and ME. Chagot and M Bourguet and Y Abel and G Terral and C Maurizy and C Aigueperse and F Georgescauld and F Vandermoere and R Saint-Fort and I Behm-Ansmant and B Charpentier and B Pradet-Balade and C Verheggen and E Bertrand and P Meyer and S Cianférani and X Manival and M Quinternet}, year = {2018}, date = {2018-01-01}, journal = {Structure}, volume = {26}, number = {9}, pages = {1196-1209.e8.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Hernandez-Alba, O; Wagner-Rousset, E; Beck, A; Cianférani, S Native Mass Spectrometry, Ion Mobility, and Collision-Induced Unfolding for Conformational Characterization of IgG4 Monoclonal Antibodies. Article de journal Anal Chem., 90 (15), p. 8865-8872., 2018. @article{617, title = {Native Mass Spectrometry, Ion Mobility, and Collision-Induced Unfolding for Conformational Characterization of IgG4 Monoclonal Antibodies.}, author = {O Hernandez-Alba and E Wagner-Rousset and A Beck and S Cianférani}, year = {2018}, date = {2018-01-01}, journal = {Anal Chem.}, volume = {90}, number = {15}, pages = {8865-8872.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Husson, G; Delangle, A; O'Hara, J; Cianferani, S; Gervais, A; Dorsselaer, Van A; Bracewell, D; Carapito, C Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development. Article de journal Anal Chem., 90 (2), p. 1241-1247., 2018. @article{593, title = {Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development.}, author = {G Husson and A Delangle and J O'Hara and S Cianferani and A Gervais and A Van Dorsselaer and D Bracewell and C Carapito}, year = {2018}, date = {2018-01-01}, journal = {Anal Chem.}, volume = {90}, number = {2}, pages = {1241-1247.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Issa, N; Guillaumot, N; Lauret, E; Matt, N; Schaeffer-Reiss, C; Dorsselaer, Van A; Reichhart, J M; Veillard, F The Circulating Protease Persephone Is an Immune Sensor for Microbial Proteolytic Activities Upstream of the Drosophila Toll Pathway Article de journal Mol Cell, 69 (4), p. 539-550 e6., 2018, (2014/10). @article{596, title = {The Circulating Protease Persephone Is an Immune Sensor for Microbial Proteolytic Activities Upstream of the Drosophila Toll Pathway}, author = {N Issa and N Guillaumot and E Lauret and N Matt and C Schaeffer-Reiss and A Van Dorsselaer and J M Reichhart and F Veillard}, year = {2018}, date = {2018-01-01}, journal = {Mol Cell}, volume = {69}, number = {4}, pages = {539-550 e6.}, note = {2014/10}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Koniev, O; Dovgan, I; Renoux, B; Ehkirch, A; Eberova, J; S., Cianférani; Kolodych, S; Papot, S; Wagner, A Reduction-rebridging strategy for the preparation of ADPN-based antibody-drug conjugates. Article de journal Medchemcomm., 9 (5), p. 827-830., 2018. @article{612, title = {Reduction-rebridging strategy for the preparation of ADPN-based antibody-drug conjugates.}, author = {O Koniev and I Dovgan and B Renoux and A Ehkirch and J Eberova and Cianférani S. and S Kolodych and S Papot and A Wagner}, year = {2018}, date = {2018-01-01}, journal = {Medchemcomm.}, volume = {9}, number = {5}, pages = {827-830.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Maurizy, C; Quinternet, M; Abel, Y; Verheggen, C; Santo, PE.; Bourguet, M; Paiva, A; Bragantini, B; Chagot, ME.; Robert, MC.; Abeza, C; Fabre, P; Fort, P; Vandermoere, F; Sousa, P; Rain, JC.; Charpentier, B; Cianférani, S; Bandeiras, TM.; Pradet-Balade, B; Manival, X; Bertrand, E The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones. Article de journal Nat Commun., 9 (1), p. 2093., 2018. @article{602, title = {The RPAP3-Cterminal domain identifies R2TP-like quaternary chaperones.}, author = {C Maurizy and M Quinternet and Y Abel and C Verheggen and PE. Santo and M Bourguet and A Paiva and B Bragantini and ME. Chagot and MC. Robert and C Abeza and P Fabre and P Fort and F Vandermoere and P Sousa and JC. Rain and B Charpentier and S Cianférani and TM. Bandeiras and B Pradet-Balade and X Manival and E Bertrand}, year = {2018}, date = {2018-01-01}, journal = {Nat Commun.}, volume = {9}, number = {1}, pages = {2093.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mons, C; Botzanowski, T; Nikolaev, A; Hellwig, P; Cianférani, S; Lescop, E; Bouton, C; Golinelli-Cohen, MP. The H2O2-Resistant Fe-S Redox Switch MitoNEET Acts as a pH Sensor To Repair Stress-Damaged Fe-S Protein. Article de journal Biochemistry, 57 (38), p. 5616-5628., 2018. @article{611, title = {The H2O2-Resistant Fe-S Redox Switch MitoNEET Acts as a pH Sensor To Repair Stress-Damaged Fe-S Protein.}, author = {C Mons and T Botzanowski and A Nikolaev and P Hellwig and S Cianférani and E Lescop and C Bouton and MP. Golinelli-Cohen}, year = {2018}, date = {2018-01-01}, journal = {Biochemistry}, volume = {57}, number = {38}, pages = {5616-5628.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mori, M; Kovalenko, L; Malancona, S; Saladini, F; Forni, De D; Pires, M; Humbert, N; Real, E; Botzanowski, T; Cianférani, S; Giannini, A; Lang, MC. Dasso; Cugia, G; Poddesu, B; Lori, F; Zazzi, M; Harper, S; Summa, V; Mely, Y; Botta, M Structure-Based Identification of HIV-1 Nucleocapsid Protein Inhibitors Active against Wild-Type and Drug-Resistant HIV-1 Strains. Article de journal ACS Chem Biol., 13 (1), p. 253-266., 2018. @article{594, title = {Structure-Based Identification of HIV-1 Nucleocapsid Protein Inhibitors Active against Wild-Type and Drug-Resistant HIV-1 Strains.}, author = {M Mori and L Kovalenko and S Malancona and F Saladini and D De Forni and M Pires and N Humbert and E Real and T Botzanowski and S Cianférani and A Giannini and MC. Dasso Lang and G Cugia and B Poddesu and F Lori and M Zazzi and S Harper and V Summa and Y Mely and M Botta}, year = {2018}, date = {2018-01-01}, journal = {ACS Chem Biol.}, volume = {13}, number = {1}, pages = {253-266.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Muller, L; Fornecker, L; Chion, M; Dorsselaer, Van A; Cianférani, S; Rabilloud, T; Carapito, C Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics. Article de journal Sci Rep., 8 (1), p. 8260, 2018, (2015/08). @article{598, title = {Extended investigation of tube-gel sample preparation: a versatile and simple choice for high throughput quantitative proteomics.}, author = {L Muller and L Fornecker and M Chion and A Van Dorsselaer and S Cianférani and T Rabilloud and C Carapito}, year = {2018}, date = {2018-01-01}, journal = {Sci Rep.}, volume = {8}, number = {1}, pages = {8260}, note = {2015/08}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ploton, M; Mazuy, C; Gheeraert, C; Dubois, V; Berthier, A; Chevalier-Dubois, J; Diemer, H; Cianférani, S; Strub, JM.; Helleboid-Chapman, A; Eeckhoute, J; Staels, B; Lefebvre, P The Nuclear Bile Acid Receptor FXR is a PKA- and FOXA2-Sensitive Activator of Fasting Hepatic Gluconeogenesis Article de journal Journal of Hepatology, 2018, (2011/30). @article{609, title = {The Nuclear Bile Acid Receptor FXR is a PKA- and FOXA2-Sensitive Activator of Fasting Hepatic Gluconeogenesis}, author = {M Ploton and C Mazuy and C Gheeraert and V Dubois and A Berthier and J Chevalier-Dubois and H Diemer and S Cianférani and JM. Strub and A Helleboid-Chapman and J Eeckhoute and B Staels and P Lefebvre}, year = {2018}, date = {2018-01-01}, journal = {Journal of Hepatology}, note = {2011/30}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Prudent, R; Demoncheaux, N; Diemer, H; Collin-Faure, V; Kapur, R; Paublant, F; Lafanechère, L; Cianférani, S; Rabilloud, T A quantitative proteomic analysis of cofilin phosphorylation in myeloid cells and its modulation using the LIM kinase inhibitor Pyr1. Article de journal PLoS One., 13 (12), p. e0208979., 2018. @article{624, title = {A quantitative proteomic analysis of cofilin phosphorylation in myeloid cells and its modulation using the LIM kinase inhibitor Pyr1.}, author = {R Prudent and N Demoncheaux and H Diemer and V Collin-Faure and R Kapur and F Paublant and L Lafanechère and S Cianférani and T Rabilloud}, year = {2018}, date = {2018-01-01}, journal = {PLoS One.}, volume = {13}, number = {12}, pages = {e0208979.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Salaam, J; Tabti, L; Bahamyirou, S; Lecointre, A; Alba, Hernandez O; Jeannin, O; Camerel, F; Cianférani, S; Bentouhami, E; Nonat, AM.; Charbonnière, LJ. Formation of Mono- and Polynuclear Luminescent Lanthanide Complexes based on the Coordination of Preorganized Phosphonated Pyridines. Article de journal Inorg Chem., 57 (10), p. 6095-6106., 2018. @article{603, title = {Formation of Mono- and Polynuclear Luminescent Lanthanide Complexes based on the Coordination of Preorganized Phosphonated Pyridines.}, author = {J Salaam and L Tabti and S Bahamyirou and A Lecointre and O Hernandez Alba and O Jeannin and F Camerel and S Cianférani and E Bentouhami and AM. Nonat and LJ. Charbonnière}, year = {2018}, date = {2018-01-01}, journal = {Inorg Chem.}, volume = {57}, number = {10}, pages = {6095-6106.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Tascher, G; Gerbaix, M; Maes, P; Chazarin, B; Ghislin, S; Antropova, E; Vassilieva, G; Ouzren-Zarhloul, N; Gauquelin-Koch, G; Vico, L; Frippiat, JP.; Bertile, F Analysis of femurs from mice embarked on board BION-M1 biosatellite reveals a decrease in immune cell development, including B cells, after 1 wk of recovery on Earth. Article de journal FASEB J., Dec 6:fj201801463R. , 2018. @article{625, title = {Analysis of femurs from mice embarked on board BION-M1 biosatellite reveals a decrease in immune cell development, including B cells, after 1 wk of recovery on Earth.}, author = {G Tascher and M Gerbaix and P Maes and B Chazarin and S Ghislin and E Antropova and G Vassilieva and N Ouzren-Zarhloul and G Gauquelin-Koch and L Vico and JP. Frippiat and F Bertile}, year = {2018}, date = {2018-01-01}, journal = {FASEB J.}, volume = {Dec 6:fj201801463R.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
van Tran, N; Muller, L; Ross, RL.; Lestini, R; Létoquart, J; Ulryck, N; Limbach, PA.; de Crécy-Lagard, V; Cianférani, S; Graille, M Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes. Article de journal Nucleic Acids Res., 46 (16), p. 8483-8499., 2018. @article{618, title = {Evolutionary insights into Trm112-methyltransferase holoenzymes involved in translation between archaea and eukaryotes.}, author = {N van Tran and L Muller and RL. Ross and R Lestini and J Létoquart and N Ulryck and PA. Limbach and V de Crécy-Lagard and S Cianférani and M Graille}, year = {2018}, date = {2018-01-01}, journal = {Nucleic Acids Res.}, volume = {46}, number = {16}, pages = {8483-8499.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Weinsanto, I; Laux-Biehlmann, A; Mouheiche, J; Maduna, T; Delalande, F; Chavant, V; Gabel, F; Darbon, P; Charlet, A; Poisbeau, P; Lamshöft, M; Dorsselaer, Van A; Cianferani, S; Parat, MO.; Goumon, Y Stable isotope-labelled morphine to study in vivo central and peripheral morphine glucuronidation and brain transport in tolerant mice. Article de journal Br J Pharmacol., 175 (19), p. 3844-3856., 2018. @article{613, title = {Stable isotope-labelled morphine to study in vivo central and peripheral morphine glucuronidation and brain transport in tolerant mice.}, author = {I Weinsanto and A Laux-Biehlmann and J Mouheiche and T Maduna and F Delalande and V Chavant and F Gabel and P Darbon and A Charlet and P Poisbeau and M Lamshöft and A Van Dorsselaer and S Cianferani and MO. Parat and Y Goumon}, year = {2018}, date = {2018-01-01}, journal = {Br J Pharmacol.}, volume = {175}, number = {19}, pages = {3844-3856.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Weinsanto, I; Mouheiche, J; Laux-Biehlmann, A; Delalande, F; Marquette, A; Chavant, V; Gabel, F; Cianferani, S; Charlet, A; Parat, MO.; Goumon, Y Morphine Binds Creatine Kinase B and Inhibits Its Activity. Article de journal Front Cell Neurosci., p. 12:464, 2018. @article{623, title = {Morphine Binds Creatine Kinase B and Inhibits Its Activity.}, author = {I Weinsanto and J Mouheiche and A Laux-Biehlmann and F Delalande and A Marquette and V Chavant and F Gabel and S Cianferani and A Charlet and MO. Parat and Y Goumon}, year = {2018}, date = {2018-01-01}, journal = {Front Cell Neurosci.}, pages = {12:464}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Salvi, Daniel; Bournais, Sylvain; Moyet, Lucas; Bouchnak, Imen; Kuntz, Marcel; Bruley, Christophe; Rolland, Norbert AT_CHLORO: The First Step When Looking for Information About Subplastidial Localization of Proteins Article de journal Methods in Molecular Biology (Clifton, N.J.), 1829 , p. 395–406, 2018, ISSN: 1940-6029. @article{salvi_at_chloro:_2018, title = {AT_CHLORO: The First Step When Looking for Information About Subplastidial Localization of Proteins}, author = {Daniel Salvi and Sylvain Bournais and Lucas Moyet and Imen Bouchnak and Marcel Kuntz and Christophe Bruley and Norbert Rolland}, doi = {10.1007/978-1-4939-8654-5_26}, issn = {1940-6029}, year = {2018}, date = {2018-01-01}, journal = {Methods in Molecular Biology (Clifton, N.J.)}, volume = {1829}, pages = {395--406}, abstract = {Plastids contain several key subcompartments. The two limiting envelope membranes (inner and outer membrane of the plastid envelope with an intermembrane space between), an aqueous phase (stroma), and an internal membrane system terms (thylakoids) formed of flat compressed vesicles (grana) and more light structures (lamellae). The thylakoid vesicles delimit another discrete soluble compartment, the thylakoid lumen. AT_CHLORO ( http://at-chloro.prabi.fr/at_chloro/ ) is a unique database supplying information about the subplastidial localization of proteins. It was created from simultaneous proteomic analyses targeted to the main subcompartments of the chloroplast from Arabidopsis thaliana (i.e., envelope, stroma, thylakoid) and to the two subdomains of thylakoid membranes (i.e., grana and stroma lamellae). AT_CHLORO assembles several complementary information (MS-based experimental data, curated functional annotations and subplastidial localization, links to other public databases and references) which give a comprehensive overview of the current knowledge about the subplastidial localization and the function of chloroplast proteins, with a specific attention given to chloroplast envelope proteins.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Plastids contain several key subcompartments. The two limiting envelope membranes (inner and outer membrane of the plastid envelope with an intermembrane space between), an aqueous phase (stroma), and an internal membrane system terms (thylakoids) formed of flat compressed vesicles (grana) and more light structures (lamellae). The thylakoid vesicles delimit another discrete soluble compartment, the thylakoid lumen. AT_CHLORO ( http://at-chloro.prabi.fr/at_chloro/ ) is a unique database supplying information about the subplastidial localization of proteins. It was created from simultaneous proteomic analyses targeted to the main subcompartments of the chloroplast from Arabidopsis thaliana (i.e., envelope, stroma, thylakoid) and to the two subdomains of thylakoid membranes (i.e., grana and stroma lamellae). AT_CHLORO assembles several complementary information (MS-based experimental data, curated functional annotations and subplastidial localization, links to other public databases and references) which give a comprehensive overview of the current knowledge about the subplastidial localization and the function of chloroplast proteins, with a specific attention given to chloroplast envelope proteins. |
Govin, Jérôme; Barral, Sophie; Morozumi, Yuichi; Hoghoughi, Naghmeh; Buchou, Thierry; Rousseaux, Sophie; Khochbin, Saadi Characterization of Post-Meiotic Male Germ Cell Genome Organizational States Article de journal Methods in Molecular Biology (Clifton, N.J.), 1832 , p. 293–307, 2018, ISSN: 1940-6029. @article{govin_characterization_2018, title = {Characterization of Post-Meiotic Male Germ Cell Genome Organizational States}, author = {Jérôme Govin and Sophie Barral and Yuichi Morozumi and Naghmeh Hoghoughi and Thierry Buchou and Sophie Rousseaux and Saadi Khochbin}, doi = {10.1007/978-1-4939-8663-7_16}, issn = {1940-6029}, year = {2018}, date = {2018-01-01}, journal = {Methods in Molecular Biology (Clifton, N.J.)}, volume = {1832}, pages = {293--307}, abstract = {Dramatic and unique genome reorganizations accompany the differentiation of haploid male germ cells, characterized by a gradual loss of the vast majority of histones leading to a final tight compaction of the genome by protamines. Despite being essential for procreation and the life cycle, the mechanisms driving the transformation of nucleosomes into nucleoprotamines remain poorly understood. To address this issue, our laboratory has developed a number of specific approaches, ranging from the purification of spermatogenic cells at specific stages, the analysis of chromatin transitional states, the functional characterization of histone variants, histone-replacing proteins and their chaperones. This chapter will detail all related relevant techniques with a particular emphasis on methods allowing the functional studies of histone variants and the genome organizational states associated with the studied histones in spermatogenic cells undergoing histone-to-protamine exchange.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Dramatic and unique genome reorganizations accompany the differentiation of haploid male germ cells, characterized by a gradual loss of the vast majority of histones leading to a final tight compaction of the genome by protamines. Despite being essential for procreation and the life cycle, the mechanisms driving the transformation of nucleosomes into nucleoprotamines remain poorly understood. To address this issue, our laboratory has developed a number of specific approaches, ranging from the purification of spermatogenic cells at specific stages, the analysis of chromatin transitional states, the functional characterization of histone variants, histone-replacing proteins and their chaperones. This chapter will detail all related relevant techniques with a particular emphasis on methods allowing the functional studies of histone variants and the genome organizational states associated with the studied histones in spermatogenic cells undergoing histone-to-protamine exchange. |
D'Atri, V; Causon, T; Hernandez-Alba, O; Mutabazi, A; Veuthey, JL.; Cianférani, S; Guillarme, D Adding a new separation dimension to MS and LC-MS: What is the utility of ion mobility spectrometry? Article de journal J Sep Sci., 41 (1), p. 20-67, 2018. @article{RN1296, title = {Adding a new separation dimension to MS and LC-MS: What is the utility of ion mobility spectrometry?}, author = {V D'Atri and T Causon and O Hernandez-Alba and A Mutabazi and JL. Veuthey and S Cianférani and D Guillarme}, doi = {10.1002/jssc.201700919}, year = {2018}, date = {2018-01-01}, journal = {J Sep Sci.}, volume = {41}, number = {1}, pages = {20-67}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
2017 |
Bouguenina, H; Salaun, D; Mangon, A; Muller, L; Baudelet, E; Camoin, L; Tachibana, T; Cianférani, S; Audebert, S; Verdier-Pinard, P; Badache, A EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions Article de journal Proc Natl Acad Sci U S A., 114 (50), p. E10687-E10696, 2017. @article{RN1309, title = {EB1-binding-myomegalin protein complex promotes centrosomal microtubules functions}, author = {H Bouguenina and D Salaun and A Mangon and L Muller and E Baudelet and L Camoin and T Tachibana and S Cianférani and S Audebert and P Verdier-Pinard and A Badache}, doi = {10.1073/pnas.1705682114}, year = {2017}, date = {2017-12-12}, journal = {Proc Natl Acad Sci U S A.}, volume = {114}, number = {50}, pages = {E10687-E10696}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Erales, Jenny; Marchand, Virginie; Panthu, Baptiste; Gillot, Sandra; Belin, Stéphane; Ghayad, Sandra E; Garcia, Maxime; Laforêts, Florian; Marcel, Virginie; Baudin-Baillieu, Agnès; Bertin, Pierre; Couté, Yohann; Adrait, Annie; Meyer, Mélanie; Therizols, Gabriel; Yusupov, Marat; Namy, Olivier; Ohlmann, Théophile; Motorin, Yuri; Catez, Frédéric; Diaz, Jean-Jacques Evidence for rRNA 2'-O-methylation plasticity: Control of intrinsic translational capabilities of human ribosomes Article de journal Proceedings of the National Academy of Sciences of the United States of America, 114 (49), p. 12934–12939, 2017, ISSN: 1091-6490. @article{erales_evidence_2017, title = {Evidence for rRNA 2'-O-methylation plasticity: Control of intrinsic translational capabilities of human ribosomes}, author = {Jenny Erales and Virginie Marchand and Baptiste Panthu and Sandra Gillot and Stéphane Belin and Sandra E Ghayad and Maxime Garcia and Florian Laforêts and Virginie Marcel and Agnès Baudin-Baillieu and Pierre Bertin and Yohann Couté and Annie Adrait and Mélanie Meyer and Gabriel Therizols and Marat Yusupov and Olivier Namy and Théophile Ohlmann and Yuri Motorin and Frédéric Catez and Jean-Jacques Diaz}, doi = {10.1073/pnas.1707674114}, issn = {1091-6490}, year = {2017}, date = {2017-12-01}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {114}, number = {49}, pages = {12934--12939}, abstract = {Ribosomal RNAs (rRNAs) are main effectors of messenger RNA (mRNA) decoding, peptide-bond formation, and ribosome dynamics during translation. Ribose 2'-O-methylation (2'-O-Me) is the most abundant rRNA chemical modification, and displays a complex pattern in rRNA. 2'-O-Me was shown to be essential for accurate and efficient protein synthesis in eukaryotic cells. However, whether rRNA 2'-O-Me is an adjustable feature of the human ribosome and a means of regulating ribosome function remains to be determined. Here we challenged rRNA 2'-O-Me globally by inhibiting the rRNA methyl-transferase fibrillarin in human cells. Using RiboMethSeq, a nonbiased quantitative mapping of 2'-O-Me, we identified a repertoire of 2'-O-Me sites subjected to variation and demonstrate that functional domains of ribosomes are targets of 2'-O-Me plasticity. Using the cricket paralysis virus internal ribosome entry site element, coupled to in vitro translation, we show that the intrinsic capability of ribosomes to translate mRNAs is modulated through a 2'-O-Me pattern and not by nonribosomal actors of the translational machinery. Our data establish rRNA 2'-O-Me plasticity as a mechanism providing functional specificity to human ribosomes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Ribosomal RNAs (rRNAs) are main effectors of messenger RNA (mRNA) decoding, peptide-bond formation, and ribosome dynamics during translation. Ribose 2'-O-methylation (2'-O-Me) is the most abundant rRNA chemical modification, and displays a complex pattern in rRNA. 2'-O-Me was shown to be essential for accurate and efficient protein synthesis in eukaryotic cells. However, whether rRNA 2'-O-Me is an adjustable feature of the human ribosome and a means of regulating ribosome function remains to be determined. Here we challenged rRNA 2'-O-Me globally by inhibiting the rRNA methyl-transferase fibrillarin in human cells. Using RiboMethSeq, a nonbiased quantitative mapping of 2'-O-Me, we identified a repertoire of 2'-O-Me sites subjected to variation and demonstrate that functional domains of ribosomes are targets of 2'-O-Me plasticity. Using the cricket paralysis virus internal ribosome entry site element, coupled to in vitro translation, we show that the intrinsic capability of ribosomes to translate mRNAs is modulated through a 2'-O-Me pattern and not by nonribosomal actors of the translational machinery. Our data establish rRNA 2'-O-Me plasticity as a mechanism providing functional specificity to human ribosomes. |
Arvin-Berod, Marie; Desroches-Castan, Agnès; Bonte, Simon; Brugière, Sabine; Couté, Yohann; Guyon, Laurent; Feige, Jean-Jacques; Baussanne, Isabelle; Demeunynck, Martine Indolizine-Based Scaffolds as Efficient and Versatile Tools: Application to the Synthesis of Biotin-Tagged Antiangiogenic Drugs Article de journal ACS Omega, 2 (12), p. 9221–9230, 2017, ISSN: 2470-1343. @article{arvin-berod_indolizine-based_2017, title = {Indolizine-Based Scaffolds as Efficient and Versatile Tools: Application to the Synthesis of Biotin-Tagged Antiangiogenic Drugs}, author = {Marie Arvin-Berod and Agnès Desroches-Castan and Simon Bonte and Sabine Brugière and Yohann Couté and Laurent Guyon and Jean-Jacques Feige and Isabelle Baussanne and Martine Demeunynck}, url = {https://doi.org/10.1021/acsomega.7b01184}, doi = {10.1021/acsomega.7b01184}, issn = {2470-1343}, year = {2017}, date = {2017-12-01}, urldate = {2018-02-22}, journal = {ACS Omega}, volume = {2}, number = {12}, pages = {9221--9230}, abstract = {We describe the design and optimization of polyfunctional scaffolds based on a fluorescent indolizine core derivatized with various orthogonal groups (amines, esters, oximes, alkynes, etc.). To show one application as tools in biology, the scaffold was used to prepare drug–biotin conjugates that were then immobilized onto avidin-agarose for affinity chromatography. More specifically, the antiangiogenic drug COB223, whose mechanism of action remained unclear, was chosen as a proof-of-concept drug. The drug-selective discrimination of proteins observed after elution of the cell lysates through the affinity columns, functionalized either with the biologically active COB223 or a structurally related inactive analogue (COB236), is a clear indication that the presence of the indolizine core does not limit drug–protein interaction and confirms the usefulness of the indolizine scaffold. Furthermore, the separation of COB223-interacting proteins from human placental extracts unveiled unanticipated protein targets belonging to the family of regulatory RNA-binding proteins, which opens the way to new hypotheses on the mode of action of this antiangiogenic drug.}, keywords = {}, pubstate = {published}, tppubtype = {article} } We describe the design and optimization of polyfunctional scaffolds based on a fluorescent indolizine core derivatized with various orthogonal groups (amines, esters, oximes, alkynes, etc.). To show one application as tools in biology, the scaffold was used to prepare drug–biotin conjugates that were then immobilized onto avidin-agarose for affinity chromatography. More specifically, the antiangiogenic drug COB223, whose mechanism of action remained unclear, was chosen as a proof-of-concept drug. The drug-selective discrimination of proteins observed after elution of the cell lysates through the affinity columns, functionalized either with the biologically active COB223 or a structurally related inactive analogue (COB236), is a clear indication that the presence of the indolizine core does not limit drug–protein interaction and confirms the usefulness of the indolizine scaffold. Furthermore, the separation of COB223-interacting proteins from human placental extracts unveiled unanticipated protein targets belonging to the family of regulatory RNA-binding proteins, which opens the way to new hypotheses on the mode of action of this antiangiogenic drug. |
Cheng, Daian; Deobagkar-Lele, Mukta; Zvezdova, Ekaterina; Choi, Seeyoung; Uehara, Shoji; Baup, Delphine; Bennett, Sophia C; Bull, Katherine R; Crockford, Tanya L; Ferry, Helen; Warzecha, Claude; è, Marl; de Peredo, Anne Gonzalez; Lesourne, Renaud; Anzilotti, Consuelo; Love, Paul E; Cornall, Richard J Themis2 lowers the threshold for B cell activation during positive selection Article de journal Nature immunology, 18 (2), p. 205–213, 2017. @article{Cheng2017, title = {Themis2 lowers the threshold for B cell activation during positive selection}, author = {Daian Cheng and Mukta Deobagkar-Lele and Ekaterina Zvezdova and Seeyoung Choi and Shoji Uehara and Delphine Baup and Sophia C Bennett and Katherine R Bull and Tanya L Crockford and Helen Ferry and Claude Warzecha and Marl{è}ne Marcellin and Anne Gonzalez de Peredo and Renaud Lesourne and Consuelo Anzilotti and Paul E Love and Richard J Cornall}, doi = {10.1038/ni.3642}, year = {2017}, date = {2017-12-01}, journal = {Nature immunology}, volume = {18}, number = {2}, pages = {205--213}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Roitel, Olivier; Bonnard, Lionel; Stella, Alexandre; Schiltz, Odile; Maurice, Delphine; ë, Ga; Jacquenet, Sandrine; Favrot, Claude; Bihain, Bernard E; Couturier, Nicolas Detection of IgE-reactive proteins in hydrolysed dog foods Article de journal Veterinary dermatology, 28 (6), p. 589–e143, 2017. @article{Roitel2017, title = {Detection of IgE-reactive proteins in hydrolysed dog foods}, author = {Olivier Roitel and Lionel Bonnard and Alexandre Stella and Odile Schiltz and Delphine Maurice and Ga{ë}l Douchin and Sandrine Jacquenet and Claude Favrot and Bernard E Bihain and Nicolas Couturier}, doi = {10.1111/vde.12473}, year = {2017}, date = {2017-12-01}, journal = {Veterinary dermatology}, volume = {28}, number = {6}, pages = {589--e143}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Carapito, C; Duek, P; Macron, C; Seffals, M; Rondel, K; Delalande, F; Lindskog, C; Freour, T; Vandenbrouck, Y; Lane, L; Pineau, C Validating missing proteins in human sperm cells by targeted mass spectrometry- and antibody-based methods Article de journal J Proteome Res, 16 (12), p. 4340-4351, 2017. @article{RN1299, title = {Validating missing proteins in human sperm cells by targeted mass spectrometry- and antibody-based methods}, author = {C Carapito and P Duek and C Macron and M Seffals and K Rondel and F Delalande and C Lindskog and T Freour and Y Vandenbrouck and L Lane and C Pineau}, doi = {10.1021/acs.jproteome.7b00374}, year = {2017}, date = {2017-12-01}, journal = {J Proteome Res}, volume = {16}, number = {12}, pages = {4340-4351}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Grillon, A; Westermann, B; Cantero, P; Jaulhac, B; Voordouw, M J; Kapps, D; Collin, E; Barthel, C; Ehret-Sabatier, L; Boulanger, N Identification of Borrelia protein candidates in mouse skin for potential diagnosis of disseminated Lyme borreliosis Article de journal Sci Rep., 7 (1), p. 16719, 2017. @article{RN1297, title = {Identification of Borrelia protein candidates in mouse skin for potential diagnosis of disseminated Lyme borreliosis}, author = {A Grillon and B Westermann and P Cantero and B Jaulhac and M J Voordouw and D Kapps and E Collin and C Barthel and L Ehret-Sabatier and N Boulanger}, doi = {10.1038/s41598-017-16749-9}, year = {2017}, date = {2017-12-01}, journal = {Sci Rep.}, volume = {7}, number = {1}, pages = {16719}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Charbonnel, C; Niazi, Khan A; Elvira-Matelor, E; Nowak, E; Zytnicki, M; de Bures, A; Jobet, E; Opsomer, A; Shamandi, N; Nowotny, M; Carapito, C; Reichheld, J P; Vaucheret, H; Saez-Vasquez, J The siRNA suppressor RTL1 is redox-regulated through glutathionylation of a conserved cysteine in the doublestranded-RNA-binding domain Article de journal Nucleic Acids Res., 45 (20), p. 11891-11907, 2017. @article{RN1301, title = {The siRNA suppressor RTL1 is redox-regulated through glutathionylation of a conserved cysteine in the doublestranded-RNA-binding domain}, author = {C Charbonnel and A Khan Niazi and E Elvira-Matelor and E Nowak and M Zytnicki and A de Bures and E Jobet and A Opsomer and N Shamandi and M Nowotny and C Carapito and J P Reichheld and H Vaucheret and J Saez-Vasquez}, doi = {10.1093/nar/gkx820}, year = {2017}, date = {2017-11-16}, journal = {Nucleic Acids Res.}, volume = {45}, number = {20}, pages = {11891-11907}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Aillaud, Chrystelle; Bosc, Christophe; Peris, Leticia; Bosson, Anouk; Heemeryck, Pierre; Dijk, Juliette Van; Friec, Julien Le; Boulan, Benoit; Vossier, Frédérique; Sanman, Laura E; Syed, Salahuddin; Amara, Neri; Couté, Yohann; Lafanechère, Laurence; Denarier, Eric; Delphin, Christian; Pelletier, Laurent; Humbert, Sandrine; Bogyo, Matthew; Andrieux, Annie; Rogowski, Krzysztof; Moutin, Marie-Jo Vasohibins/SVBP are tubulin carboxypeptidases (TCP) that regulate neuron differentiation Article de journal Science (New York, N.Y.), 2017, ISSN: 1095-9203. @article{aillaud_vasohibins/svbp_2017, title = {Vasohibins/SVBP are tubulin carboxypeptidases (TCP) that regulate neuron differentiation}, author = {Chrystelle Aillaud and Christophe Bosc and Leticia Peris and Anouk Bosson and Pierre Heemeryck and Juliette Van Dijk and Julien Le Friec and Benoit Boulan and Frédérique Vossier and Laura E Sanman and Salahuddin Syed and Neri Amara and Yohann Couté and Laurence Lafanechère and Eric Denarier and Christian Delphin and Laurent Pelletier and Sandrine Humbert and Matthew Bogyo and Annie Andrieux and Krzysztof Rogowski and Marie-Jo Moutin}, doi = {10.1126/science.aao4165}, issn = {1095-9203}, year = {2017}, date = {2017-11-01}, journal = {Science (New York, N.Y.)}, abstract = {Reversible detyrosination of α-tubulin is crucial to microtubule dynamics and functions and defects have been implicated in cancer, brain disorganization, and cardiomyopathies. The identity of the tubulin tyrosine carboxypeptidase (TCP) responsible for detyrosination has remained unclear. We used chemical proteomics with a potent unique irreversible inhibitor to show that the major brain TCP is a complex of vasohibin-1 (VASH1) with the Small Vasohibin Binding Protein (SVBP). VASH1 and its homolog VASH2, when complexed with SVBP, exhibited robust and specific Tyr/Phe carboxypeptidase activity on microtubules. Knock down of vasohibins or SVBP and/or inhibitor addition in cultured neurons reduced detyrosinated α-tubulin levels and caused severe differentiation defects. Furthermore, knock down of vasohibins disrupted neuronal migration in developing mouse neocortex. Thus vasohibin/SVBP complexes represent long sought TCP enzymes.}, keywords = {}, pubstate = {published}, tppubtype = {article} } Reversible detyrosination of α-tubulin is crucial to microtubule dynamics and functions and defects have been implicated in cancer, brain disorganization, and cardiomyopathies. The identity of the tubulin tyrosine carboxypeptidase (TCP) responsible for detyrosination has remained unclear. We used chemical proteomics with a potent unique irreversible inhibitor to show that the major brain TCP is a complex of vasohibin-1 (VASH1) with the Small Vasohibin Binding Protein (SVBP). VASH1 and its homolog VASH2, when complexed with SVBP, exhibited robust and specific Tyr/Phe carboxypeptidase activity on microtubules. Knock down of vasohibins or SVBP and/or inhibitor addition in cultured neurons reduced detyrosinated α-tubulin levels and caused severe differentiation defects. Furthermore, knock down of vasohibins disrupted neuronal migration in developing mouse neocortex. Thus vasohibin/SVBP complexes represent long sought TCP enzymes. |
Yamchi, Ahad; Ben, Cécile; Rossignol, Michel; Zareie, Sayed Reza; Mirlohi, Aghafakhr; Sayed-Tabatabaei, Badraldin Ebrahim; Pichereaux, Carole; Sarrafi, Ahmad; Rickauer, Martina; Gentzbittel, Laurent Cellular microbiology, p. e12796, 2017. @article{Yamchi2017, title = {Proteomics analysis of Medicago truncatula response to infection by the phytopathogenic bacterium Ralstonia solanacearum points to jasmonate and salicylate defence pathways.}, author = {Ahad Yamchi and Cécile Ben and Michel Rossignol and Sayed Reza Zareie and Aghafakhr Mirlohi and Badraldin Ebrahim Sayed-Tabatabaei and Carole Pichereaux and Ahmad Sarrafi and Martina Rickauer and Laurent Gentzbittel}, doi = {10.1111/cmi.12796}, year = {2017}, date = {2017-10-30}, journal = {Cellular microbiology}, pages = {e12796}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Montacié, C; Durut, N; Opsomer, A; Palm, D; Comella, P; Picart, C; Carpentier, M C; Pontvianne, F; Carapito, C; Schleiff, E; Saez-Vasquez, J Nucleolar proteome analysis and proteasomal activity assays reveal a link between nucleolus and 26S proteasome in A. thaliana Article de journal Front Plant Sci., 8 , p. 1815., 2017. @article{RN1302, title = {Nucleolar proteome analysis and proteasomal activity assays reveal a link between nucleolus and 26S proteasome in A. thaliana}, author = {C Montacié and N Durut and A Opsomer and D Palm and P Comella and C Picart and M C Carpentier and F Pontvianne and C Carapito and E Schleiff and J Saez-Vasquez}, doi = {10.3389/fpls.2017.01815}, year = {2017}, date = {2017-10-20}, journal = {Front Plant Sci.}, volume = {8}, pages = {1815.}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Terral, G; CHAMPION, T; Debaene, F; Colas, O; Bourguet, M; WAGNER-ROUSSET, E; Corvaia, N; Beck, A; Cianferani, S Epitope characterization of anti-JAM-A antibodies using orthogonal mass spectrometry and surface plasmon resonance approaches Article de journal MAbs., 9 (8), p. 1317-1326, 2017. @article{RN1294, title = {Epitope characterization of anti-JAM-A antibodies using orthogonal mass spectrometry and surface plasmon resonance approaches}, author = {G Terral and T CHAMPION and F Debaene and O Colas and M Bourguet and E WAGNER-ROUSSET and N Corvaia and A Beck and S Cianferani}, doi = {10.1080/19420862.2017.1380762}, year = {2017}, date = {2017-10-16}, journal = {MAbs.}, volume = {9}, number = {8}, pages = {1317-1326}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Radu, L; Schoenwetter, E; Braun, C; Marcoux, J; Koelmel, W; Schmitt, DR.; Kuper, J; Cianférani, S; Egly, JM.; Poterszman, A; Kisker, C The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH Article de journal Nucleic Acids Res., 45 (18), p. 10872-10883, 2017. @article{RN1293, title = {The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH}, author = {L Radu and E Schoenwetter and C Braun and J Marcoux and W Koelmel and DR. Schmitt and J Kuper and S Cianférani and JM. Egly and A Poterszman and C Kisker}, doi = {10.1093/nar/gkx743}, year = {2017}, date = {2017-10-13}, journal = {Nucleic Acids Res.}, volume = {45}, number = {18}, pages = {10872-10883}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Chiaradia, Laura; Lefebvre, Cyril; Parra, Julien; Marcoux, Julien; Burlet-Schiltz, Odile; Etienne, Gilles; Tropis, Maryelle; Daffé, Mamadou Dissecting the mycobacterial cell envelope and defining the composition of the native mycomembrane Article de journal Scientific Reports, 7 (1), p. 12807, 2017. @article{Chiaradia2017, title = {Dissecting the mycobacterial cell envelope and defining the composition of the native mycomembrane}, author = {Laura Chiaradia and Cyril Lefebvre and Julien Parra and Julien Marcoux and Odile Burlet-Schiltz and Gilles Etienne and Maryelle Tropis and Mamadou Daffé}, doi = {10.1038/s41598-017-12718-4}, year = {2017}, date = {2017-10-01}, journal = {Scientific Reports}, volume = {7}, number = {1}, pages = {12807}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Guéguéniat, J; Dupin, AF.; Stojko, J; Beaurepaire, L; Cianférani, S; Mackereth, CD.; Minvielle-Sébastia, L; Fribourg, S Distinct roles of Pcf11 zinc-binding domains in pre-mRNA 3'-end processing Article de journal Nucleic Acids Res., 45 (17), p. 10115-10131, 2017. @article{RN1292, title = {Distinct roles of Pcf11 zinc-binding domains in pre-mRNA 3'-end processing}, author = {J Guéguéniat and AF. Dupin and J Stojko and L Beaurepaire and S Cianférani and CD. Mackereth and L Minvielle-Sébastia and S Fribourg}, doi = {10.1093/nar/gkx674}, year = {2017}, date = {2017-09-29}, journal = {Nucleic Acids Res.}, volume = {45}, number = {17}, pages = {10115-10131}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Sorigué, Damien; Légeret, Bertrand; Cuiné, Stéphan; Blangy, Stéphanie; Moulin, Solène; Billon, Emmanuelle; Richaud, Pierre; Brugière, Sabine; Couté, Yohann; Nurizzo, Didier; Müller, Pavel; Brettel, Klaus; Pignol, David; Arnoux, Pascal; Li-Beisson, Yonghua; Peltier, Gilles; Beisson, Fred An algal photoenzyme converts fatty acids to hydrocarbons Article de journal Science (New York, N.Y.), 357 (6354), p. 903-907, 2017. @article{sorigue_algal_2017, title = {An algal photoenzyme converts fatty acids to hydrocarbons}, author = {Damien Sorigué and Bertrand Légeret and Stéphan Cuiné and Stéphanie Blangy and Solène Moulin and Emmanuelle Billon and Pierre Richaud and Sabine Brugière and Yohann Couté and Didier Nurizzo and Pavel Müller and Klaus Brettel and David Pignol and Pascal Arnoux and Yonghua Li-Beisson and Gilles Peltier and Fred Beisson}, doi = {10.1126/science.aan6349}, year = {2017}, date = {2017-09-01}, journal = {Science (New York, N.Y.)}, volume = {357}, number = {6354}, pages = {903-907}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Baxter, P N W; Karmazin, L; DeCian, A; Varnek, A; Gisselbrecht, J -P; Strub, J -M; Cianférani, S A Direct One-Pot Synthesis of Asymmetric Dehydrobenzopyrido-[12]annulenes and Their Physicochemical Properties Article de journal Eur. J. Org. Chem., 2017 (31), p. 4625–4632, 2017. @article{RN1291, title = {A Direct One-Pot Synthesis of Asymmetric Dehydrobenzopyrido-[12]annulenes and Their Physicochemical Properties}, author = {P N W Baxter and L Karmazin and A DeCian and A Varnek and J -P Gisselbrecht and J -M Strub and S Cianférani}, doi = {10.1002/ejoc.201700700}, year = {2017}, date = {2017-08-23}, journal = {Eur. J. Org. Chem.}, volume = {2017}, number = {31}, pages = {4625–4632}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Maes, Pauline; Donadio-Andréi, Sandrine; Louwagie, Mathilde; Couté, Yohann; Picard, Guillaume; Lacoste, Claire; Bruley, Christophe; Garin, Jérôme; Ichai, Philippe; Faivre, Jamila; Jaquinod, Michel; Brun, Virginie Introducing plasma/serum glycodepletion for the targeted proteomics analysis of cytolysis biomarkers Article de journal Talanta, 170 , p. 473-480, 2017. @article{maes_introducing_2017, title = {Introducing plasma/serum glycodepletion for the targeted proteomics analysis of cytolysis biomarkers}, author = {Pauline Maes and Sandrine Donadio-Andréi and Mathilde Louwagie and Yohann Couté and Guillaume Picard and Claire Lacoste and Christophe Bruley and Jérôme Garin and Philippe Ichai and Jamila Faivre and Michel Jaquinod and Virginie Brun}, doi = {10.1016/j.talanta.2017.04.042}, year = {2017}, date = {2017-08-01}, journal = {Talanta}, volume = {170}, pages = {473-480}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Alonso, Henar; Parra, Julien; Malaga, Wladimir; Payros, Delphine; Liu, Chia-Fang; Berrone, Céline; Robert, Camille; Meunier, Etienne; Burlet-Schiltz, Odile; è, Michel Rivi; Guilhot, Christophe Protein O-mannosylation deficiency increases LprG-associated lipoarabinomannan release by Mycobacterium tuberculosis and enhances the TLR2-associated inflammatory response Article de journal Scientific Reports, 7 (1), p. 7913, 2017. @article{Alonso2017, title = {Protein O-mannosylation deficiency increases LprG-associated lipoarabinomannan release by Mycobacterium tuberculosis and enhances the TLR2-associated inflammatory response}, author = {Henar Alonso and Julien Parra and Wladimir Malaga and Delphine Payros and Chia-Fang Liu and Céline Berrone and Camille Robert and Etienne Meunier and Odile Burlet-Schiltz and Michel Rivi{è}re and Christophe Guilhot}, doi = {10.1038/s41598-017-08489-7}, year = {2017}, date = {2017-08-01}, journal = {Scientific Reports}, volume = {7}, number = {1}, pages = {7913}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
F, Duguet; M, Locard-Paulet; M, Marcellin; K, Chaoui; I, Bernard; O, Andreoletti; R, Lesourne; O, Burlet-Schiltz; de A, Gonzalez Peredo; A, Saoudi Proteomic analysis of regulatory T cells reveals the importance of Themis1 in the control of their suppressive function Article de journal Mol Cell Proteomics, 16 , p. 1416-1432, 2017. @article{F2017, title = { Proteomic analysis of regulatory T cells reveals the importance of Themis1 in the control of their suppressive function}, author = {Duguet F and Locard-Paulet M and Marcellin M and Chaoui K and Bernard I and Andreoletti O and Lesourne R and Burlet-Schiltz O and Gonzalez de Peredo A and Saoudi A}, doi = {10.1074/mcp.M116.062745}, year = {2017}, date = {2017-08-01}, journal = {Mol Cell Proteomics}, volume = {16}, pages = {1416-1432}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dalzon, B; Aude-Garcia, C; Collin-Faure, V; Diemer, H; Béal, D; Dussert, F; Fenel, D; Schoehn, G; Cianférani, S; Carrière, M; Rabilloud, T Differential proteomics highlights macrophage-specific responses to amorphous silica nanoparticles Article de journal Nanoscale, 9 (27), p. 9641-9658, 2017. @article{RN1286, title = {Differential proteomics highlights macrophage-specific responses to amorphous silica nanoparticles}, author = {B Dalzon and C Aude-Garcia and V Collin-Faure and H Diemer and D Béal and F Dussert and D Fenel and G Schoehn and S Cianférani and M Carrière and T Rabilloud}, doi = {10.1039/c7nr02140b}, year = {2017}, date = {2017-07-13}, journal = {Nanoscale}, volume = {9}, number = {27}, pages = {9641-9658}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Bartoli, J; My, L; Belmudes, Lucid; Couté, Yohann; Viala, J P; Bouveret, E The Long Hunt for pssR-Looking for a Phospholipid Synthesis Transcriptional Regulator, Finding the Ribosome Article de journal Journal of Bacteriology, 199 (14), 2017. @article{bartoli_long_2017, title = {The Long Hunt for pssR-Looking for a Phospholipid Synthesis Transcriptional Regulator, Finding the Ribosome}, author = {J Bartoli and L My and Lucid Belmudes and Yohann Couté and J P Viala and E Bouveret}, doi = {10.1128/JB.00202-17}, year = {2017}, date = {2017-07-01}, journal = {Journal of Bacteriology}, volume = {199}, number = {14}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Miller, Marion; Chen, Aichun; Gobert, Vanessa; Augé, Benoit; Beau, Mathilde; Burlet-Schiltz, Odile; Haenlin, Marc; Waltzer, Lucas Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis Article de journal PLoS Genetics, 13 (7), p. e1006932, 2017. @article{Miller2017, title = {Control of RUNX-induced repression of Notch signaling by MLF and its partner DnaJ-1 during Drosophila hematopoiesis}, author = {Marion Miller and Aichun Chen and Vanessa Gobert and Benoit Augé and Mathilde Beau and Odile Burlet-Schiltz and Marc Haenlin and Lucas Waltzer}, editor = {Claude Desplan}, doi = {10.1371/journal.pgen.1006932}, year = {2017}, date = {2017-07-01}, journal = {PLoS Genetics}, volume = {13}, number = {7}, pages = {e1006932}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
C, Dozier; L, Mazzolini; C, Cénac; C, Froment; O, Burlet-Schiltz; A, Besson; S, Manenti CyclinD-CDK4/6 complexes phosphorylate CDC25A and regulate its stability Article de journal Oncogene, 36 (26), p. 3781-3788, 2017. @article{C2017, title = {CyclinD-CDK4/6 complexes phosphorylate CDC25A and regulate its stability}, author = {Dozier C and Mazzolini L and Cénac C and Froment C and Burlet-Schiltz O and Besson A and Manenti S}, doi = {10.1038/onc.2016.506 }, year = {2017}, date = {2017-06-29}, journal = {Oncogene}, volume = {36}, number = {26}, pages = {3781-3788}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mohideen-Abdul, K; Tazibt, K; Bourguet, M; Hazemann, I; Lebars, I; Takacs, M; Cianférani, S; Klaholz, B P; Moras, D; Billas, I M L Importance of the Sequence-Directed DNA Shape for Specific Binding Site Recognition by the Estrogen-Related Receptor Article de journal Front. Endocrinol., 8 , p. 140, 2017. @article{RN1284, title = {Importance of the Sequence-Directed DNA Shape for Specific Binding Site Recognition by the Estrogen-Related Receptor}, author = {K Mohideen-Abdul and K Tazibt and M Bourguet and I Hazemann and I Lebars and M Takacs and S Cianférani and B P Klaholz and D Moras and I M L Billas}, doi = {10.3389/fendo.2017.00140}, year = {2017}, date = {2017-06-20}, journal = {Front. Endocrinol.}, volume = {8}, pages = {140}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Gloaguen, Pauline; Bournais, Sylvain; Alban, Claude; Ravanel, Stéphane; Seigneurin-Berny, Daphné; Matringe, Michel; Tardif, Marianne; Kuntz, Marcel; Ferro, Myriam; Bruley, Christophe; Rolland, Norbert; Vandenbrouck, Yves; Curien, Gilles ChloroKB: A Web Application for the Integration of Knowledge Related to Chloroplast Metabolic Network Article de journal Plant Physiology, 174 (2), p. 922-934, 2017. @article{gloaguen_chlorokb:_2017, title = {ChloroKB: A Web Application for the Integration of Knowledge Related to Chloroplast Metabolic Network}, author = {Pauline Gloaguen and Sylvain Bournais and Claude Alban and Stéphane Ravanel and Daphné Seigneurin-Berny and Michel Matringe and Marianne Tardif and Marcel Kuntz and Myriam Ferro and Christophe Bruley and Norbert Rolland and Yves Vandenbrouck and Gilles Curien}, doi = {10.1104/pp.17.00242}, year = {2017}, date = {2017-06-01}, journal = {Plant Physiology}, volume = {174}, number = {2}, pages = {922-934}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Vizcaíno, Juan Antonio; Walzer, Mathias; Jiménez, Rafael C; Bittremieux, Wout; Bouyssié, David; Carapito, Christine; Corrales, Fernando; Ferro, Myriam; Heck, Albert J R; Horvatovich, Peter; Hubalek, Martin; Lane, Lydie; Laukens, Kris; Levander, Fredrik; Lisacek, Frederique; Novak, Petr; Palmblad, Magnus; Piovesan, Damiano; Pühler, Alfred; Schwämmle, Veit; Valkenborg, Dirk; van Rijswijk, Merlijn; Vondrasek, Jiri; Eisenacher, Martin; Martens, Lennart; Kohlbacher, Oliver A community proposal to integrate proteomics activities in ELIXIR Article de journal F1000Research, 6 , p. 875, 2017. @article{Vizcaino2017, title = {A community proposal to integrate proteomics activities in ELIXIR}, author = {Juan Antonio Vizcaíno and Mathias Walzer and Rafael C Jiménez and Wout Bittremieux and David Bouyssié and Christine Carapito and Fernando Corrales and Myriam Ferro and Albert J R Heck and Peter Horvatovich and Martin Hubalek and Lydie Lane and Kris Laukens and Fredrik Levander and Frederique Lisacek and Petr Novak and Magnus Palmblad and Damiano Piovesan and Alfred Pühler and Veit Schwämmle and Dirk Valkenborg and Merlijn van Rijswijk and Jiri Vondrasek and Martin Eisenacher and Lennart Martens and Oliver Kohlbacher}, doi = {10.12688/f1000research.11751.1}, year = {2017}, date = {2017-06-01}, journal = {F1000Research}, volume = {6}, pages = {875}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Botzanowski, T; Erb, S; Hernandez-Alba, O; Ehkirch, A; Colas, O; Wagner-Rousset, E; Rabuka, D; Beck, A; Drake, PM.; Cianférani, S Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates Article de journal MAbs, 9 (5), p. 801-811, 2017. @article{RN1289, title = {Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates}, author = {T Botzanowski and S Erb and O Hernandez-Alba and A Ehkirch and O Colas and E Wagner-Rousset and D Rabuka and A Beck and PM. Drake and S Cianférani}, doi = {10.1080/19420862.2017.1316914}, year = {2017}, date = {2017-05-25}, journal = {MAbs}, volume = {9}, number = {5}, pages = {801-811}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Wagner-Rousset, E; Fekete, S; Morel-Chevillet, L; Colas, O; Corvaïa, N; Cianférani, S; Guillarme, D; Beck, A Development of a fast workflow to screen the charge variants of therapeutic antibodies Article de journal J Chromatogr A, 1498 , p. 147-154, 2017. @article{RN1290, title = {Development of a fast workflow to screen the charge variants of therapeutic antibodies}, author = {E Wagner-Rousset and S Fekete and L Morel-Chevillet and O Colas and N Corvaïa and S Cianférani and D Guillarme and A Beck}, doi = {10.1016/j.chroma.2017.02.065}, year = {2017}, date = {2017-05-19}, journal = {J Chromatogr A}, volume = {1498}, pages = {147-154}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Dovgan, I; Ursuegui, S; Erb, S; Michel, C; Kolodych, S; Cianférani, S; Wagner, A Acyl Fluorides: Fast, Efficient, and Versatile Lysine-Based Protein Conjugation via Plug-and-Play Strategy Article de journal Bioconjug Chem., 28 (5), p. 1452-1457, 2017. @article{RN1287, title = {Acyl Fluorides: Fast, Efficient, and Versatile Lysine-Based Protein Conjugation via Plug-and-Play Strategy}, author = {I Dovgan and S Ursuegui and S Erb and C Michel and S Kolodych and S Cianférani and A Wagner}, doi = {10.1021/acs.bioconjchem.7b00141 }, year = {2017}, date = {2017-05-17}, journal = {Bioconjug Chem.}, volume = {28}, number = {5}, pages = {1452-1457}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Argemi, X; Prévost, G; Riegel, P; Keller, D; Meyer, N; Baldeyrou, M; Douiri, N; Lefebvre, N; Meghit, K; Ronde Oustau, C; Christmann, D; Cianférani, S; Strub, JM. ; Hansmann, Y VISLISI trial, a prospective clinical study allowing identification of a new metalloprotease and putative virulence factor from Staphylococcus lugdunensis Article de journal Clin Microbiol Infect., 23 (5), p. 334.e1–334.e8, 2017. @article{RN149, title = {VISLISI trial, a prospective clinical study allowing identification of a new metalloprotease and putative virulence factor from Staphylococcus lugdunensis}, author = {Argemi, X. and Prévost, G. and Riegel, P. and Keller, D. and Meyer, N. and Baldeyrou, M. and Douiri, N. and Lefebvre, N. and Meghit, K. and Ronde Oustau, C. and Christmann, D. and Cianférani, S. and Strub, JM. and Hansmann, Y.}, doi = {10.1016/j.cmi.2016.12.018}, year = {2017}, date = {2017-05-02}, journal = {Clin Microbiol Infect.}, volume = {23}, number = {5}, pages = {334.e1–334.e8}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Mietton, Flore; Ferri, Elena; Champleboux, Morgane; Zala, Ninon; Maubon, Danièle; Zhou, Yingsheng; Harbut, Mike; Spittler, Didier; Garnaud, Cécile; Courçon, Marie; Chauvel, Murielle; d'Enfert, Christophe ; Kashemirov, Boris A; Hull, Mitchell; Cornet, Muriel; McKenna, Charles E; Govin, Jérôme; Petosa, Carlo Selective BET bromodomain inhibition as an antifungal therapeutic strategy Article de journal Nature Communications, 8 , p. 15482, 2017. @article{mietton_selective_2017, title = {Selective BET bromodomain inhibition as an antifungal therapeutic strategy}, author = {Flore Mietton and Elena Ferri and Morgane Champleboux and Ninon Zala and Danièle Maubon and Yingsheng Zhou and Mike Harbut and Didier Spittler and Cécile Garnaud and Marie Courçon and Murielle Chauvel and Christophe {d'Enfert} and Boris A Kashemirov and Mitchell Hull and Muriel Cornet and Charles E McKenna and Jérôme Govin and Carlo Petosa}, doi = {10.1038/ncomms15482}, year = {2017}, date = {2017-05-01}, journal = {Nature Communications}, volume = {8}, pages = {15482}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Willems, Sander; Bouyssié, David; David, Matthieu; Locard-Paulet, Marie; Mechtler, Karl; Schwämmle, Veit; Uszkoreit, Julian; Vaudel, Marc; Dorfer, Viktoria Proceedings of the EuBIC Winter School 2017. Article de journal Journal of proteomics, 161 , p. 78–80, 2017. @article{Willems2017, title = {Proceedings of the EuBIC Winter School 2017.}, author = {Sander Willems and David Bouyssié and Matthieu David and Marie Locard-Paulet and Karl Mechtler and Veit Schwämmle and Julian Uszkoreit and Marc Vaudel and Viktoria Dorfer}, doi = {10.1016/j.jprot.2017.04.001}, year = {2017}, date = {2017-05-01}, journal = {Journal of proteomics}, volume = {161}, pages = {78--80}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
C, Carel; J, Marcoux; V, Réat; J, Parra; G, Latgé; F, Laval; P, Demange; O, Burlet-Schiltz; A, Milon; M, Daffé; MG, Tropis; MA, Renault Identification of specific posttranslational O-mycoloylations mediating protein targeting to the mycomembrane Article de journal Proc Natl Acad Sci U S A, 114 (16), p. 4231-4236, 2017. @article{C2017b, title = {Identification of specific posttranslational O-mycoloylations mediating protein targeting to the mycomembrane}, author = {Carel C and Marcoux J and Réat V and Parra J and Latgé G and Laval F and Demange P and Burlet-Schiltz O and Milon A and Daffé M and Tropis MG and Renault MA}, doi = {10.1073/pnas.1617888114}, year = {2017}, date = {2017-04-18}, journal = {Proc Natl Acad Sci U S A}, volume = {114}, number = {16}, pages = {4231-4236}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Ehrmann, FR.; Stojko, J; Metz, A; Debaene, F; Barandun, LJ.; Heine, A; Diederich, F; Cianférani, S; Reuter, K; Klebe, G Soaking suggests Article de journal PLoS One, 12 (4), p. e0175723, 2017. @article{RN1288, title = {Soaking suggests }, author = {FR. Ehrmann and J Stojko and A Metz and F Debaene and LJ. Barandun and A Heine and F Diederich and S Cianférani and K Reuter and G Klebe}, doi = {10.1371/journal.pone.0175723}, year = {2017}, date = {2017-04-18}, journal = {PLoS One}, volume = {12}, number = {4}, pages = {e0175723}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Fabre, Elisabeth; Jeudy, Sandra; Santini, Sébastien; Legendre, Matthieu; Trauchessec, Mathieu; Couté, Yohann; Claverie, Jean-Michel; Abergel, Chantal Noumeavirus replication relies on a transient remote control of the host nucleus Article de journal Nature Communications, 8 , p. 15087, 2017. @article{fabre_noumeavirus_2017, title = {Noumeavirus replication relies on a transient remote control of the host nucleus}, author = {Elisabeth Fabre and Sandra Jeudy and Sébastien Santini and Matthieu Legendre and Mathieu Trauchessec and Yohann Couté and Jean-Michel Claverie and Chantal Abergel}, doi = {10.1038/ncomms15087}, year = {2017}, date = {2017-04-01}, journal = {Nature Communications}, volume = {8}, pages = {15087}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Duriez, Elodie; Masselon, Christophe D; Mesmin, Cédric; Court, Magali; Demeure, Kevin; Allory, Yves; Malats, Núria; Matondo, Mariette; Radvanyi, François; Garin, Jérôme; Domon, Bruno Large-Scale SRM Screen of Urothelial Bladder Cancer Candidate Biomarkers in Urine Article de journal Journal of Proteome Research, 16 (4), p. 1617–1631, 2017. @article{duriez_large-scale_2017, title = {Large-Scale SRM Screen of Urothelial Bladder Cancer Candidate Biomarkers in Urine}, author = {Elodie Duriez and Christophe D Masselon and Cédric Mesmin and Magali Court and Kevin Demeure and Yves Allory and Núria Malats and Mariette Matondo and François Radvanyi and Jérôme Garin and Bruno Domon}, doi = {10.1021/acs.jproteome.6b00979}, year = {2017}, date = {2017-04-01}, journal = {Journal of Proteome Research}, volume = {16}, number = {4}, pages = {1617--1631}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Matondo, Mariette; è, Marl; Chaoui, Karima; Bousquet-Dubouch, Marie Pierre; Gonzalez-de-Peredo, Anne; Monsarrat, Bernard; Burlet-Schiltz, Odile Determination of differentially regulated proteins upon proteasome inhibition in AML cell lines by the combination of large-scale and targeted quantitative proteomics Article de journal Proteomics, 17 (7), p. 1600089, 2017. @article{Matondo2017, title = {Determination of differentially regulated proteins upon proteasome inhibition in AML cell lines by the combination of large-scale and targeted quantitative proteomics}, author = {Mariette Matondo and Marl{è}ne Marcellin and Karima Chaoui and Marie Pierre Bousquet-Dubouch and Anne Gonzalez-de-Peredo and Bernard Monsarrat and Odile Burlet-Schiltz}, doi = {10.1002/pmic.201600089}, year = {2017}, date = {2017-04-01}, journal = {Proteomics}, volume = {17}, number = {7}, pages = {1600089}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Carel, Clément; Marcoux, Julien; Réat, Valérie; Parra, Julien; Latgé, Guillaume; ç, Fran; Demange, Pascal; Burlet-Schiltz, Odile; Milon, Alain; Daffé, Mamadou; Tropis, Maryelle G; Renault, Marie A M Identification of specific posttranslational O -mycoloylations mediating protein targeting to the mycomembrane Article de journal Proceedings of the National Academy of Sciences, 114 (16), p. 4231–4236, 2017. @article{Carel2017, title = {Identification of specific posttranslational O -mycoloylations mediating protein targeting to the mycomembrane}, author = {Clément Carel and Julien Marcoux and Valérie Réat and Julien Parra and Guillaume Latgé and Fran{ç}oise Laval and Pascal Demange and Odile Burlet-Schiltz and Alain Milon and Mamadou Daffé and Maryelle G Tropis and Marie A M Renault}, doi = {10.1073/pnas.1617888114}, year = {2017}, date = {2017-04-01}, journal = {Proceedings of the National Academy of Sciences}, volume = {114}, number = {16}, pages = {4231--4236}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Rosnoblet, Claire; è, Hervé B; Blanchard, Cécile; Pichereaux, Carole; Besson-Bard, Angélique; Aimé, Sébastien; Wendehenne, David Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco. Article de journal Plant, cell & environment, 40 (4), p. 491–508, 2017. @article{Rosnoblet2017, title = {Functional characterization of the chaperon-like protein Cdc48 in cryptogein-induced immune response in tobacco.}, author = {Claire Rosnoblet and Hervé B{è}gue and Cécile Blanchard and Carole Pichereaux and Angélique Besson-Bard and Sébastien Aimé and David Wendehenne}, doi = {10.1111/pce.12686}, year = {2017}, date = {2017-04-01}, journal = {Plant, cell & environment}, volume = {40}, number = {4}, pages = {491--508}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
Barral, Sophie; Morozumi, Yuichi; Tanaka, Hiroki; Montellier, Emilie; Govin, Jérôme; de Dieuleveult, Maud ; Charbonnier, Guillaume; Couté, Yohann; Puthier, Denis; Buchou, Thierry; Boussouar, Fayçal; Urahama, Takashi; Fenaille, François; Curtet, Sandrine; Héry, Patrick; Fernandez-Nunez, Nicolas; Shiota, Hitoshi; Gérard, Matthieu; Rousseaux, Sophie; Kurumizaka, Hitoshi; Khochbin, Saadi Histone Variant Ħ2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells Article de journal Molecular Cell, 66 (1), p. 89-101.e8, 2017. @article{barral_histone_2017, title = {Histone Variant Ħ2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells}, author = {Sophie Barral and Yuichi Morozumi and Hiroki Tanaka and Emilie Montellier and Jérôme Govin and Maud {de Dieuleveult} and Guillaume Charbonnier and Yohann Couté and Denis Puthier and Thierry Buchou and Fayçal Boussouar and Takashi Urahama and François Fenaille and Sandrine Curtet and Patrick Héry and Nicolas Fernandez-Nunez and Hitoshi Shiota and Matthieu Gérard and Sophie Rousseaux and Hitoshi Kurumizaka and Saadi Khochbin}, doi = {10.1016/j.molcel.2017.02.025}, year = {2017}, date = {2017-04-01}, journal = {Molecular Cell}, volume = {66}, number = {1}, pages = {89-101.e8}, keywords = {}, pubstate = {published}, tppubtype = {article} } |
