Samples (2 μg of yeast cell lysate + different spiked levels of UPS1) were analyzed in triplicate by nanoLC–MS/MS on an LTQ-Orbitrap Velos mass spectrometer. For more information on samples preparation and LC-MS/MS analyses, please refer to Ramus et al., J Proteomics. 2016 Jan 30;132:51-62. doi: 10.1016/j.jprot.2015.11.011.
| SAMPLE | RAW File | Mascot dat File | mzDB File |
|---|---|---|---|
| 2µg levure UPS1 100fmol- Replicat 1 | OEMMA121101_61b.raw | F083068.dat | OEMMA121101_61b.mzdb |
| 2µg levure UPS1 100fmol- Replicat 2 | OEMMA121101_63b.raw | F083069.dat | OEMMA121101_63b.mzdb |
| 2µg levure UPS1 100fmol- Replicat 3 | OEMMA121101_65b.raw | F083070.dat | OEMMA121101_65b.mzdb |
| 2µg levure UPS1 10fmol – Replicat 1 | OEMMA121101_36b.raw | F083064.dat | OEMMA121101_36b.mzdb |
| 2µg levure UPS1 10fmol – Replicat 2 | OEMMA121101_38b.raw | F083066.dat | OEMMA121101_38b.mzdb |
| 2µg levure UPS1 10fmol – Replicat 3 | OEMMA121101_40b.raw | F083067.dat | OEMMA121101_40b.mzdb |
- 2µg levure UPS1 10fmol replicats: mascot files only, mascot and mzDB files (raw files are also available for those that want to reprocess the data)
- 2µg levure UPS1 100fmol replicats: mascot files only, mascot and mzDB files (raw files)
Short protocol description: Peaklist were generated using the Extract_msn.exe macro provided with Xcalibur. Peaklists were submitted to Mascot database searches (version 2.5.1). ESI-TRAP was chosen as the instrument, trypsin/P as the enzyme and 2 missed cleavages were allowed. Precursor and fragment mass error tolerances were set at 5 ppm and 0.8 Da, respectively. Peptide variable modifications allowed during the search were: acetyl (Protein N-ter), oxidation (M), whereas carbamidomethyl (C) was set as fixed modification. Databases used were yeast database from UniprotKB (S_cerevisiae_ 20121108.fasta, 7798 sequences), a compiled database containing the UPS1 human sequences (48 sequences) and the corresponding reversed databases in order to calculate FDR.