Conducting Research and Services
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Who we are
ProFI is a national infrastructure in Proteomics and provide tools, methods and services to the academic and private partners in all fields of biology.
At the heart of life sciences and health lies a need to decrypt the major cellular mechanisms. This description necessarily involves characterisation of the molecular actors, in particular proteins, involved in these mechanisms. The ProFI infrastructure focuses its R&D activity on the development of tools and methods to allow increasingly specific characterisation of the proteins making up biological systems. The stakes being played for with this strategy are considerable, since thanks to the efforts made by the major proteomics centres and to advances in instrumentation and calculation capacities, proteomics analysis should eventually allow creation of a repertoire of all of the proteins contained in a biological sample, along with their abundance and the interactions which link them. This analysis should also make it possible to “visualise” almost the whole sequence of proteins, providing access to posttranslational modifications. Within ProFI, this information is sought in the context of the study of the dynamics of biological systems: protein complexes, intracellular compartments, cells, or even tissues.
In addition to the R&D efforts undertaken, ProFI’s mission is to offer the community a high-quality service in terms of proteomics analysis as part of collaborations or paid-for services, and to help innervate the very numerous French proteomics platforms by sharing know-how and tools developed by the infrastructure.
ProFI facilities are distributed over three sites in France
The “Exploring the Dynamics of Proteomes” (EDyP) team is an academic research laboratory from the CEA, Inserm and Grenoble Alpes University.
EDyP team develops analytical methods based on mass-spectrometry to solve challenging questions in basic biology and translational medicine. Particularly, EDyP research activity aims at searching for new biomarkers and deciphering molecular pathology using label-free and targeted proteomics approaches. Computational methods and software tools are also specifically developed for the analysis of proteomics datasets. EDyP team also operates a core facility (EDyP Service) which provides a broad range of proteomics services for academic and industrial partners.
The BioOrganic Mass Spectrometry laboratory (LSMBO) belongs to the Institut Plurisciplinaire Hubert Curien (IPHC : http://www.iphc.cnrs.fr/) of the University of Strasbourg. The LSMBO is hosting an IBiSA-certified proteomic platform (Strasbourg Grand Est Proteomic Platform) and is the Strasbourg node of the French Proteomic Infrastructure (ProFI). The LSMBO is a 35 scientists team that has a 30 year-experience in the structural study of peptides and proteins by mass spectrometry. The laboratory is renowned for developing new methods in proteomic analysis, bioinformatics and structural mass spectrometry for the analysis of protein/protein interactions in large complexes. Besides that, long term collaborations with several pharmaceutical companies cover topics such as full characterization of natural and/or recombinant proteins used in human therapy, toxicoproteomics, protein/drug interaction.
Contact: Sarah Cianférani
The “Proteomics and Mass Spectrometry of Biomolecules” research group (PMSB) is one of the 17 teams of the Institute of Pharmacology and Structural Biology (IPBS) in Toulouse, which is a mixed CNRS/Paul Sabatier University research unit. It is part of the Structural Biology and Biophysics department of the Institute. The team’s objective is to develop innovative proteomics strategies to gain new insights into protein function, regulation and dynamic interactions to solve fundamental biological questions. It is composed of 20 highly qualified people with complementary skills in mass spectrometry, biochemistry and bioinformatics. Research projects benefit from state-of-the-art instrumentation in a context of quality assurance certification (ISO 9001 since 2006 and NF X 50-900 since 2014).
Major research projects are devoted to the understanding of biological processes involved mainly in cancer and inflammation using mass spectrometry and proteomics. These projects also include methodological developments in proteomic strategies, like the identification of low-abundance proteins, quantitative proteomics, the characterization of post-translational modifications, and the analysis of protein complexes. Overall, the work of the team led to the publication of more than 80 articles since 2001 in high impact journals.
Contact: Odile Schiltz