Husson, G.; Delangle, A.; O’Hara, J.; Cianferani, S.; Gervais, A.; Van Dorsselaer, A.; Bracewell, D.; Carapito, C., Dual Data-Independent Acquisition Approach Combining Global HCP Profiling and Absolute Quantification of Key Impurities during Bioprocess Development. Anal Chem. 2018 90 (2) :1241-1247.
The MS Top 3-ID-DIA method allows global Host Cell Proteins (HCP) profiling and absolute quantification of key HCP within a single analysis, and therefore constitutes a promising alternative to classical ELISA kits to support bioprocess development.
