Carapito C, Duek P, Macron C, Seffals M, Rondel K, Delalande F, Lindskog C, Fréour, Vandenbrouck Y, Lane L, Pineau C. Validating Missing Proteins in Human Sperm Cells by Targeted Mass-Spectrometry- and Antibody-based Methods. J Proteome Res. 2017.
In the context of the C-HPP worldwide effort, ProFI as a partner of the French-Swiss consortium, contributed to the targeted search of 38 theoretically detectable missing proteins (MPs) from chromosomes 2 and 14 various sperm fractions from a total of 15 healthy donors. The development of LC−PRM assays (with heavy labeled synthetic peptides) allows to identify 12 MPs with two or more peptides and 3 with one peptide after extensive SDS-PAGE fractionation. Eight MPs were further validated by immunohistochemistry on human testes sections and cytochemistry on sperm smears. As in-depth analysis of these MPs may identify potential new candidates in the context of human idiopathic infertilities, we anticipate that these results will be of interest to the reproductive biology community.